P53 gene snp investigation in squamous cell oesophageal carcinom
Squamous cell cancer (ESCC) and adenocarcinoma (EADC) are the two main subtypes of oesophageal cancer. Genetic alterations associated with the development of oesophageal cancer are often observed. The aim of this study was to identify p53 gene polymorphisms in several patients with oesophageal squam...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
"Alexandru Ioan Cuza" University of Iași
2010-06-01
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| Series: | Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara |
| Online Access: | http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/763 |
| Summary: | Squamous cell cancer (ESCC) and adenocarcinoma (EADC) are the two main subtypes of oesophageal cancer.
Genetic alterations associated with the development of oesophageal cancer are often observed. The aim of this study was
to identify p53 gene polymorphisms in several patients with oesophageal squamous cell carcinoma. Genomic DNA was
isolated from normal-, tumor- and blood-samples from patients with ESCC. PCR amplification of two p53 gene
fragments was performed using two sets of primers DNA samples were sequenced and then analyzed with BLAST
program Exons 5, 7 and 8 did not present mutations for the studied patients, but exon 6 featured one modification in two
different patients. The mutations of p53 gene can determine the modification of its proteine function and the loss of its
antioncogene role, which reflects the abnormal proliferation of the cells implicated in this type of cancer. |
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| ISSN: | 1582-3571 2248-3276 |