Microbial Communities in Methane Cycle: Modern Molecular Methods Gain Insights into Their Global Ecology
The role of methane as a greenhouse gas in the concept of global climate changes is well known. Methanogens and methanotrophs are two microbial groups which contribute to the biogeochemical methane cycle in soil, so that the total emission of CH<sub>4</sub> is the balance between its pro...
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doaj-39cd6ad34e8d4822886c62c9bd1d98bf2021-02-23T00:04:58ZengMDPI AGEnvironments2076-32982021-02-018161610.3390/environments8020016Microbial Communities in Methane Cycle: Modern Molecular Methods Gain Insights into Their Global EcologySergey Kharitonov0Mikhail Semenov1Alexander Sabrekov2Oleg Kotsyurbenko3Alena Zhelezova4Natalia Schegolkova5Soil Science Faculty, Moscow State University, Leninskie Gory 1-12, 119991 Moscow, RussiaDepartment of Soil Biology and Biochemistry, Dokuchaev Soil Science Institute, Pyzhyovskiy lane 7 Building 2, 119017 Moscow, RussiaWater Problems Institute, Russian Academy of Sciences, Gubkina st., 119333 Moscow, RussiaWater Problems Institute, Russian Academy of Sciences, Gubkina st., 119333 Moscow, RussiaDepartment of Soil Biology and Biochemistry, Dokuchaev Soil Science Institute, Pyzhyovskiy lane 7 Building 2, 119017 Moscow, RussiaSoil Science Faculty, Moscow State University, Leninskie Gory 1-12, 119991 Moscow, RussiaThe role of methane as a greenhouse gas in the concept of global climate changes is well known. Methanogens and methanotrophs are two microbial groups which contribute to the biogeochemical methane cycle in soil, so that the total emission of CH<sub>4</sub> is the balance between its production and oxidation by microbial communities. Traditional identification techniques, such as selective enrichment and pure-culture isolation, have been used for a long time to study diversity of methanogens and methanotrophs. However, these techniques are characterized by significant limitations, since only a relatively small fraction of the microbial community could be cultured. Modern molecular methods for quantitative analysis of the microbial community such as real-time PCR (Polymerase chain reaction), DNA fingerprints and methods based on high-throughput sequencing together with different “omics” techniques overcome the limitations imposed by culture-dependent approaches and provide new insights into the diversity and ecology of microbial communities in the methane cycle. Here, we review available knowledge concerning the abundances, composition, and activity of methanogenic and methanotrophic communities in a wide range of natural and anthropogenic environments. We suggest that incorporation of microbial data could fill the existing microbiological gaps in methane flux modeling, and significantly increase the predictive power of models for different environments.https://www.mdpi.com/2076-3298/8/2/16methanegreenhouse gasesmicrobial communitieshigh-throughput sequencingmcrApmoA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sergey Kharitonov Mikhail Semenov Alexander Sabrekov Oleg Kotsyurbenko Alena Zhelezova Natalia Schegolkova |
spellingShingle |
Sergey Kharitonov Mikhail Semenov Alexander Sabrekov Oleg Kotsyurbenko Alena Zhelezova Natalia Schegolkova Microbial Communities in Methane Cycle: Modern Molecular Methods Gain Insights into Their Global Ecology Environments methane greenhouse gases microbial communities high-throughput sequencing mcrA pmoA |
author_facet |
Sergey Kharitonov Mikhail Semenov Alexander Sabrekov Oleg Kotsyurbenko Alena Zhelezova Natalia Schegolkova |
author_sort |
Sergey Kharitonov |
title |
Microbial Communities in Methane Cycle: Modern Molecular Methods Gain Insights into Their Global Ecology |
title_short |
Microbial Communities in Methane Cycle: Modern Molecular Methods Gain Insights into Their Global Ecology |
title_full |
Microbial Communities in Methane Cycle: Modern Molecular Methods Gain Insights into Their Global Ecology |
title_fullStr |
Microbial Communities in Methane Cycle: Modern Molecular Methods Gain Insights into Their Global Ecology |
title_full_unstemmed |
Microbial Communities in Methane Cycle: Modern Molecular Methods Gain Insights into Their Global Ecology |
title_sort |
microbial communities in methane cycle: modern molecular methods gain insights into their global ecology |
publisher |
MDPI AG |
series |
Environments |
issn |
2076-3298 |
publishDate |
2021-02-01 |
description |
The role of methane as a greenhouse gas in the concept of global climate changes is well known. Methanogens and methanotrophs are two microbial groups which contribute to the biogeochemical methane cycle in soil, so that the total emission of CH<sub>4</sub> is the balance between its production and oxidation by microbial communities. Traditional identification techniques, such as selective enrichment and pure-culture isolation, have been used for a long time to study diversity of methanogens and methanotrophs. However, these techniques are characterized by significant limitations, since only a relatively small fraction of the microbial community could be cultured. Modern molecular methods for quantitative analysis of the microbial community such as real-time PCR (Polymerase chain reaction), DNA fingerprints and methods based on high-throughput sequencing together with different “omics” techniques overcome the limitations imposed by culture-dependent approaches and provide new insights into the diversity and ecology of microbial communities in the methane cycle. Here, we review available knowledge concerning the abundances, composition, and activity of methanogenic and methanotrophic communities in a wide range of natural and anthropogenic environments. We suggest that incorporation of microbial data could fill the existing microbiological gaps in methane flux modeling, and significantly increase the predictive power of models for different environments. |
topic |
methane greenhouse gases microbial communities high-throughput sequencing mcrA pmoA |
url |
https://www.mdpi.com/2076-3298/8/2/16 |
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