Migratory behaviour of tumour cells: a scanning electron microscopy study
BACKGROUND: Tumour cells utilize different migration strategies to invade surrounding tissues and elude anticancer treatments. It is therefore important to understand the mechanisms underlying migration process, in order to aid the development of therapies aimed at blocking the dissemination of canc...
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Istituto Superiore di Sanità
2015-06-01
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doaj-39f1b2746b7e4fe78dcc2180c4f61a612020-11-25T03:05:56ZengIstituto Superiore di SanitàAnnali dell'Istituto Superiore di Sanità0021-25712015-06-0151213914710.4415/ANN_15_02_12S0021-25712015000200012Migratory behaviour of tumour cells: a scanning electron microscopy studyGiuseppina Bozzuto0Maria Condello1Agnese Molinari2Istituto Superiore di SanitàIstituto Superiore di SanitàIstituto Superiore di SanitàBACKGROUND: Tumour cells utilize different migration strategies to invade surrounding tissues and elude anticancer treatments. It is therefore important to understand the mechanisms underlying migration process, in order to aid the development of therapies aimed at blocking the dissemination of cancer cells. AIMS: In this study tumour cell lines of different histological origin were analysed by combining 2D and 3D in vitro assays, biochemical tests and high resolution imaging by scanning electron microscopy (SEM) in order to look insight strategies adopted by tumour cells to invade extracellular matrix. RESULTS: Quantitative (computer-assisted colour camera equipped-light microscopy) and qualitative analysis (SEM) indicated that the most aggressive tumour cells adopt an "individual" behaviour. The analysis of intracellular signalling demonstrated that the highest invasive potential was associated with the activation of AKT, ERK, FAK and ERM proteins. The "individual" behaviour was positively related to the expression of VLA-2 and inversely related with the E-cadherin expression. CONCLUSIONS: The combination of 2D and 3D in vitro assays, biochemical tests and ultrastructural investigations proved to be a suitable test for the investigation of tumour cell migration and invasion. The high resolution imaging by SEM highlighted the interrelationships between cells in different migratory behaviours of tumour cells.http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S0021-25712015000200012&lng=en&tlng=enmigrationinvasiontumour cellsscanning electron microscopy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Giuseppina Bozzuto Maria Condello Agnese Molinari |
spellingShingle |
Giuseppina Bozzuto Maria Condello Agnese Molinari Migratory behaviour of tumour cells: a scanning electron microscopy study Annali dell'Istituto Superiore di Sanità migration invasion tumour cells scanning electron microscopy |
author_facet |
Giuseppina Bozzuto Maria Condello Agnese Molinari |
author_sort |
Giuseppina Bozzuto |
title |
Migratory behaviour of tumour cells: a scanning electron microscopy study |
title_short |
Migratory behaviour of tumour cells: a scanning electron microscopy study |
title_full |
Migratory behaviour of tumour cells: a scanning electron microscopy study |
title_fullStr |
Migratory behaviour of tumour cells: a scanning electron microscopy study |
title_full_unstemmed |
Migratory behaviour of tumour cells: a scanning electron microscopy study |
title_sort |
migratory behaviour of tumour cells: a scanning electron microscopy study |
publisher |
Istituto Superiore di Sanità |
series |
Annali dell'Istituto Superiore di Sanità |
issn |
0021-2571 |
publishDate |
2015-06-01 |
description |
BACKGROUND: Tumour cells utilize different migration strategies to invade surrounding tissues and elude anticancer treatments. It is therefore important to understand the mechanisms underlying migration process, in order to aid the development of therapies aimed at blocking the dissemination of cancer cells. AIMS: In this study tumour cell lines of different histological origin were analysed by combining 2D and 3D in vitro assays, biochemical tests and high resolution imaging by scanning electron microscopy (SEM) in order to look insight strategies adopted by tumour cells to invade extracellular matrix. RESULTS: Quantitative (computer-assisted colour camera equipped-light microscopy) and qualitative analysis (SEM) indicated that the most aggressive tumour cells adopt an "individual" behaviour. The analysis of intracellular signalling demonstrated that the highest invasive potential was associated with the activation of AKT, ERK, FAK and ERM proteins. The "individual" behaviour was positively related to the expression of VLA-2 and inversely related with the E-cadherin expression. CONCLUSIONS: The combination of 2D and 3D in vitro assays, biochemical tests and ultrastructural investigations proved to be a suitable test for the investigation of tumour cell migration and invasion. The high resolution imaging by SEM highlighted the interrelationships between cells in different migratory behaviours of tumour cells. |
topic |
migration invasion tumour cells scanning electron microscopy |
url |
http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S0021-25712015000200012&lng=en&tlng=en |
work_keys_str_mv |
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