A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice

• Main Authors: Jun Zhou Li, Yong Run Cao, Meng Qi Li, Wen Li Zhao, Hong Zheng Sun, Quan Zhi Zhao
• Format: Article
• Language: English
• Published: Brazilian Society of Plant Breeding 2017-06-01
• Series: Crop Breeding and Applied Biotechnology
• Subjects: Functional marker; S5-n; DEP1; multiplex PCR
• Online Access: http://www.sbmp.org.br/cbab/siscbab/uploads/bb7f427b-8033-dbec.pdf
• ISSN: 1518-7853; 1984-7033

Wide compatibility and erect panicles are important traits for heterosis and ideal plant type breeding in rice and accordingly are targets for rice yield improvement. In this study, a multiplex PCR system based on functional markers was developed to simultaneously identify genotypes of the wide compatibility allele S5-n and the erect panicle allele dep1. Forty-nine rice varieties in the Huang-Huai-Hai region of China were identified using this system. Thirty-three varieties contained the erect panicle allele dep1 and two varieties contained the wide compatibility allele S5-n. The frequency of dep1 was obviously higher than that of S5-n. The multiplex PCR method was also tested using an F2 segregating population and was found to be simple, efficient, and reliable. It will be useful in marker-assisted selection based on the wide compatibility allele S5-n and erect panicle allele dep1 to breed super rice varieties using indica and japonica heterosis.