A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice

Wide compatibility and erect panicles are important traits for heterosis and ideal plant type breeding in rice and accordingly are targets for rice yield improvement. In this study, a multiplex PCR system based on functional markers was developed to simultaneously identify genotypes of the wide comp...

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Main Authors:	Jun Zhou Li, Yong Run Cao, Meng Qi Li, Wen Li Zhao, Hong Zheng Sun, Quan Zhi Zhao
Format:	Article
Language:	English
Published:	Brazilian Society of Plant Breeding 2017-06-01
Series:	Crop Breeding and Applied Biotechnology
Subjects:	Functional marker S5-n DEP1 multiplex PCR
Online Access:	http://www.sbmp.org.br/cbab/siscbab/uploads/bb7f427b-8033-dbec.pdf

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spelling	doaj-39f326599ec34c56b5cdb4f2b6ec9bb82020-11-25T01:35:44ZengBrazilian Society of Plant BreedingCrop Breeding and Applied Biotechnology1518-78531984-70332017-06-0117324425210.1590/1984-70332017v17n3a38A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in riceJun Zhou Li0Yong Run Cao1Meng Qi Li2Wen Li Zhao3Hong Zheng Sun4Quan Zhi Zhao5Collaborative Innovation Center of Henan Grain Crops and Henan Key Laboratory of Rice Biology, Henan Agricultural University, Zhengzhou 450002, China,Collaborative Innovation Center of Henan Grain Crops and Henan Key Laboratory of Rice Biology, Henan Agricultural University, Zhengzhou 450002, China,Collaborative Innovation Center of Henan Grain Crops and Henan Key Laboratory of Rice Biology, Henan Agricultural University, Zhengzhou 450002, China,Collaborative Innovation Center of Henan Grain Crops and Henan Key Laboratory of Rice Biology, Henan Agricultural University, Zhengzhou 450002, China,Collaborative Innovation Center of Henan Grain Crops and Henan Key Laboratory of Rice Biology, Henan Agricultural University, Zhengzhou 450002, China,Collaborative Innovation Center of Henan Grain Crops and Henan Key Laboratory of Rice Biology, Henan Agricultural University, Zhengzhou 450002, China,Wide compatibility and erect panicles are important traits for heterosis and ideal plant type breeding in rice and accordingly are targets for rice yield improvement. In this study, a multiplex PCR system based on functional markers was developed to simultaneously identify genotypes of the wide compatibility allele S5-n and the erect panicle allele dep1. Forty-nine rice varieties in the Huang-Huai-Hai region of China were identified using this system. Thirty-three varieties contained the erect panicle allele dep1 and two varieties contained the wide compatibility allele S5-n. The frequency of dep1 was obviously higher than that of S5-n. The multiplex PCR method was also tested using an F2 segregating population and was found to be simple, efficient, and reliable. It will be useful in marker-assisted selection based on the wide compatibility allele S5-n and erect panicle allele dep1 to breed super rice varieties using indica and japonica heterosis.http://www.sbmp.org.br/cbab/siscbab/uploads/bb7f427b-8033-dbec.pdfFunctional markerS5-nDEP1multiplex PCR
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jun Zhou Li Yong Run Cao Meng Qi Li Wen Li Zhao Hong Zheng Sun Quan Zhi Zhao
spellingShingle	Jun Zhou Li Yong Run Cao Meng Qi Li Wen Li Zhao Hong Zheng Sun Quan Zhi Zhao A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice Crop Breeding and Applied Biotechnology Functional marker S5-n DEP1 multiplex PCR
author_facet	Jun Zhou Li Yong Run Cao Meng Qi Li Wen Li Zhao Hong Zheng Sun Quan Zhi Zhao
author_sort	Jun Zhou Li
title	A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice
title_short	A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice
title_full	A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice
title_fullStr	A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice
title_full_unstemmed	A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice
title_sort	multiplex pcr system for detection of wide compatibility allele s5-n and erect panicle allele dep1 in rice
publisher	Brazilian Society of Plant Breeding
series	Crop Breeding and Applied Biotechnology
issn	1518-7853 1984-7033
publishDate	2017-06-01
description	Wide compatibility and erect panicles are important traits for heterosis and ideal plant type breeding in rice and accordingly are targets for rice yield improvement. In this study, a multiplex PCR system based on functional markers was developed to simultaneously identify genotypes of the wide compatibility allele S5-n and the erect panicle allele dep1. Forty-nine rice varieties in the Huang-Huai-Hai region of China were identified using this system. Thirty-three varieties contained the erect panicle allele dep1 and two varieties contained the wide compatibility allele S5-n. The frequency of dep1 was obviously higher than that of S5-n. The multiplex PCR method was also tested using an F2 segregating population and was found to be simple, efficient, and reliable. It will be useful in marker-assisted selection based on the wide compatibility allele S5-n and erect panicle allele dep1 to breed super rice varieties using indica and japonica heterosis.
topic	Functional marker S5-n DEP1 multiplex PCR
url	http://www.sbmp.org.br/cbab/siscbab/uploads/bb7f427b-8033-dbec.pdf
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A multiplex PCR system for detection of wide compatibility allele S5-n and erect panicle allele dep1 in rice

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