Detection of short tandem repeats at 5 loci and amelogenin with cell-free fetal DNA as a specimen in the development of prenatal paternity diagnostic tests
Abstract Introduction A prenatal paternity test is one widely-used method of determining the paternity of an unborn child. Such tests using chorionic villus or amniocentesis may increase the risk of harm to both mother and foetus. In the present day, a prenatal paternity test using circulating cell-...
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doaj-39f6d6e9d192465f851273022e9dc9332020-11-25T00:44:21ZengSpringerOpenEgyptian Journal of Forensic Sciences2090-59392018-02-01811510.1186/s41935-018-0047-9Detection of short tandem repeats at 5 loci and amelogenin with cell-free fetal DNA as a specimen in the development of prenatal paternity diagnostic testsAgung Sosiawan0Dadik Raharjo1Indah Nuraini2Nadia Kartikasari3Alexander Patera Nugraha4Muhammad Dimas Aditya Ari5Faculty of Dental Medicine, Universitas AirlanggaPublic Health Departement, Faculty of Veterinary, Universitas AirlanggaHuman Genetic and Forensic Laboratory, Institute for Tropical Diseases, Universitas AirlanggaFaculty of Dental Medicine, Universitas AirlanggaFaculty of Dental Medicine, Universitas AirlanggaFaculty of Dental Medicine, Universitas AirlanggaAbstract Introduction A prenatal paternity test is one widely-used method of determining the paternity of an unborn child. Such tests using chorionic villus or amniocentesis may increase the risk of harm to both mother and foetus. In the present day, a prenatal paternity test using circulating cell-free fetal Deoxyribonucleic Acid (DNA) is one alternative method due to it being non-invasive and safe for both mother and foetus. Aim The aim of this study is to detect Short Tandem Repeat (STR) at 5 Loci (vWA, TH01, D13S317, D18S51, and D21S11) and amelogenin genes in circulating cell-free fetal DNA in paternity tests. Materials and methods Forty-one samples of maternal blood were obtained from pregnant woman. Circulating free fetal DNA was subsequently extracted. A paternity test was conducted using an STR test at loci vWA, TH01, D13S317, D18S51, D21S11 in circulating free fetal DNA. An analysis of the paternity test between loci > 200 bp and < 200 bp was also conducted to establish the sensitivity of the test. Results There was a significant difference between maternal blood DNA and circulating cell-free fetal DNA (p = 0.000 D13S317; p = 0.000 D21S11; p = 0.000 D18S51; p = 0.000 vWA; p = 0.000 TH01; and p = 0.000 amelogenin genes). The locus < 200 bp also had a higher sensitivity than locus > 200 bp. Conclusion Circulating free fetal DNA can be used as an alternative sample for prenatal paternity tests because of its similarity with maternal DNA.http://link.springer.com/article/10.1186/s41935-018-0047-9Early detectionCirculating free fetal DNANon-invasive techniquePrenatal paternity test |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Agung Sosiawan Dadik Raharjo Indah Nuraini Nadia Kartikasari Alexander Patera Nugraha Muhammad Dimas Aditya Ari |
spellingShingle |
Agung Sosiawan Dadik Raharjo Indah Nuraini Nadia Kartikasari Alexander Patera Nugraha Muhammad Dimas Aditya Ari Detection of short tandem repeats at 5 loci and amelogenin with cell-free fetal DNA as a specimen in the development of prenatal paternity diagnostic tests Egyptian Journal of Forensic Sciences Early detection Circulating free fetal DNA Non-invasive technique Prenatal paternity test |
author_facet |
Agung Sosiawan Dadik Raharjo Indah Nuraini Nadia Kartikasari Alexander Patera Nugraha Muhammad Dimas Aditya Ari |
author_sort |
Agung Sosiawan |
title |
Detection of short tandem repeats at 5 loci and amelogenin with cell-free fetal DNA as a specimen in the development of prenatal paternity diagnostic tests |
title_short |
Detection of short tandem repeats at 5 loci and amelogenin with cell-free fetal DNA as a specimen in the development of prenatal paternity diagnostic tests |
title_full |
Detection of short tandem repeats at 5 loci and amelogenin with cell-free fetal DNA as a specimen in the development of prenatal paternity diagnostic tests |
title_fullStr |
Detection of short tandem repeats at 5 loci and amelogenin with cell-free fetal DNA as a specimen in the development of prenatal paternity diagnostic tests |
title_full_unstemmed |
Detection of short tandem repeats at 5 loci and amelogenin with cell-free fetal DNA as a specimen in the development of prenatal paternity diagnostic tests |
title_sort |
detection of short tandem repeats at 5 loci and amelogenin with cell-free fetal dna as a specimen in the development of prenatal paternity diagnostic tests |
publisher |
SpringerOpen |
series |
Egyptian Journal of Forensic Sciences |
issn |
2090-5939 |
publishDate |
2018-02-01 |
description |
Abstract Introduction A prenatal paternity test is one widely-used method of determining the paternity of an unborn child. Such tests using chorionic villus or amniocentesis may increase the risk of harm to both mother and foetus. In the present day, a prenatal paternity test using circulating cell-free fetal Deoxyribonucleic Acid (DNA) is one alternative method due to it being non-invasive and safe for both mother and foetus. Aim The aim of this study is to detect Short Tandem Repeat (STR) at 5 Loci (vWA, TH01, D13S317, D18S51, and D21S11) and amelogenin genes in circulating cell-free fetal DNA in paternity tests. Materials and methods Forty-one samples of maternal blood were obtained from pregnant woman. Circulating free fetal DNA was subsequently extracted. A paternity test was conducted using an STR test at loci vWA, TH01, D13S317, D18S51, D21S11 in circulating free fetal DNA. An analysis of the paternity test between loci > 200 bp and < 200 bp was also conducted to establish the sensitivity of the test. Results There was a significant difference between maternal blood DNA and circulating cell-free fetal DNA (p = 0.000 D13S317; p = 0.000 D21S11; p = 0.000 D18S51; p = 0.000 vWA; p = 0.000 TH01; and p = 0.000 amelogenin genes). The locus < 200 bp also had a higher sensitivity than locus > 200 bp. Conclusion Circulating free fetal DNA can be used as an alternative sample for prenatal paternity tests because of its similarity with maternal DNA. |
topic |
Early detection Circulating free fetal DNA Non-invasive technique Prenatal paternity test |
url |
http://link.springer.com/article/10.1186/s41935-018-0047-9 |
work_keys_str_mv |
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