Size-controlled bimodal in vivo nanoprobes as near-infrared phosphors and positive contrast agents for magnetic resonance imaging
Rare-earth-doped nanoparticles (NPs), such as NaGdF4 nanocrystals doped with light-emitting rare earth ions, are promising bimodal probes that allow the integration of over 1000 nm near-infrared (OTN-NIR; NIR-II/III) fluorescence imaging and magnetic resonance imaging (MRI) of live bodies. A precise...
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doaj-39f80b28c5534d55a91e40df25b46bf62021-03-18T15:12:46ZengTaylor & Francis GroupScience and Technology of Advanced Materials1468-69961878-55142021-12-0122116017210.1080/14686996.2021.18877121887712Size-controlled bimodal in vivo nanoprobes as near-infrared phosphors and positive contrast agents for magnetic resonance imagingKyohei Okubo0Ryuta Takeda1Shuhei Murayama2Masakazu Umezawa3Masao Kamimura4Kensuke Osada5Ichio Aoki6Kohei Soga7Tokyo University of ScienceTokyo University of ScienceNational Institutes for Quantum and Radiological Science and Technology (QST)Tokyo University of ScienceTokyo University of ScienceNational Institutes for Quantum and Radiological Science and Technology (QST)National Institutes for Quantum and Radiological Science and Technology (QST)Tokyo University of ScienceRare-earth-doped nanoparticles (NPs), such as NaGdF4 nanocrystals doped with light-emitting rare earth ions, are promising bimodal probes that allow the integration of over 1000 nm near-infrared (OTN-NIR; NIR-II/III) fluorescence imaging and magnetic resonance imaging (MRI) of live bodies. A precise control of the particle size is the key factor for achieving a high signal-to-noise ratio in both NIR fluorescence and MR images and for regulating their function in the body. In this study, size-controlled NaGdF4:Yb3+, Er3+ NPs prepared by stepwise crystal growth were used for in vivo bimodal imaging. Hexagonal NaGdF4:Yb3+,Er3+ NPs coated with poly(ethylene glycol)-poly(acrylic acid) block copolymer, with hydrodynamic diameters of 15 and 45 nm, were prepared and evaluated as bimodal NPs for OTN-NIR fluorescence imaging and MRI. Their longitudinal (T1) and transverse (T2) relaxation rates at the static magnetic field strength of 1.0 T, as well as their cytotoxicity towards NIH3T3 cell lines, were evaluated and compared to study the effect of size. Using these particles, blood vessel visualization was achieved by MRI, with the highest relaxometric ratio (r1/r2) of 0.79 reported to date for NaGdF4-based nanoprobes (r1 = 19.78 mM−1 s−1), and by OTN-NIR fluorescence imaging. The results clearly demonstrate the potential of the size-controlled PEG-modified NaGdF4:Yb3+,Er3+ NPs as powerful ‘positive’ T1-weight contrast MRI agents and OTN-NIR fluorophores.http://dx.doi.org/10.1080/14686996.2021.1887712bioimagingmagnetic resonance imagingpositive contrast agentsnear-infrared fluorescencerare-earth doped ceramics nanoparticles |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kyohei Okubo Ryuta Takeda Shuhei Murayama Masakazu Umezawa Masao Kamimura Kensuke Osada Ichio Aoki Kohei Soga |
spellingShingle |
Kyohei Okubo Ryuta Takeda Shuhei Murayama Masakazu Umezawa Masao Kamimura Kensuke Osada Ichio Aoki Kohei Soga Size-controlled bimodal in vivo nanoprobes as near-infrared phosphors and positive contrast agents for magnetic resonance imaging Science and Technology of Advanced Materials bioimaging magnetic resonance imaging positive contrast agents near-infrared fluorescence rare-earth doped ceramics nanoparticles |
author_facet |
Kyohei Okubo Ryuta Takeda Shuhei Murayama Masakazu Umezawa Masao Kamimura Kensuke Osada Ichio Aoki Kohei Soga |
author_sort |
Kyohei Okubo |
title |
Size-controlled bimodal in vivo nanoprobes as near-infrared phosphors and positive contrast agents for magnetic resonance imaging |
title_short |
Size-controlled bimodal in vivo nanoprobes as near-infrared phosphors and positive contrast agents for magnetic resonance imaging |
title_full |
Size-controlled bimodal in vivo nanoprobes as near-infrared phosphors and positive contrast agents for magnetic resonance imaging |
title_fullStr |
Size-controlled bimodal in vivo nanoprobes as near-infrared phosphors and positive contrast agents for magnetic resonance imaging |
title_full_unstemmed |
Size-controlled bimodal in vivo nanoprobes as near-infrared phosphors and positive contrast agents for magnetic resonance imaging |
title_sort |
size-controlled bimodal in vivo nanoprobes as near-infrared phosphors and positive contrast agents for magnetic resonance imaging |
publisher |
Taylor & Francis Group |
series |
Science and Technology of Advanced Materials |
issn |
1468-6996 1878-5514 |
publishDate |
2021-12-01 |
description |
Rare-earth-doped nanoparticles (NPs), such as NaGdF4 nanocrystals doped with light-emitting rare earth ions, are promising bimodal probes that allow the integration of over 1000 nm near-infrared (OTN-NIR; NIR-II/III) fluorescence imaging and magnetic resonance imaging (MRI) of live bodies. A precise control of the particle size is the key factor for achieving a high signal-to-noise ratio in both NIR fluorescence and MR images and for regulating their function in the body. In this study, size-controlled NaGdF4:Yb3+, Er3+ NPs prepared by stepwise crystal growth were used for in vivo bimodal imaging. Hexagonal NaGdF4:Yb3+,Er3+ NPs coated with poly(ethylene glycol)-poly(acrylic acid) block copolymer, with hydrodynamic diameters of 15 and 45 nm, were prepared and evaluated as bimodal NPs for OTN-NIR fluorescence imaging and MRI. Their longitudinal (T1) and transverse (T2) relaxation rates at the static magnetic field strength of 1.0 T, as well as their cytotoxicity towards NIH3T3 cell lines, were evaluated and compared to study the effect of size. Using these particles, blood vessel visualization was achieved by MRI, with the highest relaxometric ratio (r1/r2) of 0.79 reported to date for NaGdF4-based nanoprobes (r1 = 19.78 mM−1 s−1), and by OTN-NIR fluorescence imaging. The results clearly demonstrate the potential of the size-controlled PEG-modified NaGdF4:Yb3+,Er3+ NPs as powerful ‘positive’ T1-weight contrast MRI agents and OTN-NIR fluorophores. |
topic |
bioimaging magnetic resonance imaging positive contrast agents near-infrared fluorescence rare-earth doped ceramics nanoparticles |
url |
http://dx.doi.org/10.1080/14686996.2021.1887712 |
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