Fractionation of the mixture obtained by the enzymatic hydrolysis of penicillin G

• Main Authors: Cascaval Dan, Oniscu Corneliu, Galaction Anca-Irina
• Format: Article
• Language: English
• Published: Association of Chemical Engineers of Serbia 2002-01-01
• Series: Hemijska Industrija
• Subjects: reactive extraction; 6-aminopenicillanic acid; penicillin G; phenylacetic acid; amberlite LA-2; di-(2-ethylhexyl) phosphoric acid
• Online Access: http://www.doiserbia.nb.rs/img/doi/0367-598X/2002/0367-598X0209386C.pdf

Using the experimental data on the individual physical and reactive extraction of 6APA with 1,2-dichlororethane, D2EHPA and Amberlite LA-2 together with data on the influence of the pH value of the aqueous phase and the Amberlite LA-2 concentration on the selective separation of 6APA, PG and PAA from a mixture, a process flow sheet for the fractionation of the solution obtained by the enzymatic hydrolysis of PG has been elaborated and applied. Thus, at pH=10, 6APA was selectively separated by four extraction stages, the overall extraction degree being 98.8%. PG was selectively extracted from the raffinate at pH=6 by three extraction stages, adjusting the molar concentration of Amberlite LA-2 to the value of the PG molar concentration for each extraction stage. The antibiotic extraction degree was 99.6%.