Vaccine Inoculation Route Modulates Early Immunity and Consequently Antigen-Specific Immune Response
Vaccination is one of the most efficient public healthcare measures to fight infectious diseases. Nevertheless, the immune mechanisms induced in vivo by vaccination are still unclear. The route of administration, an important vaccination parameter, can substantially modify the quality of the respons...
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Format: | Article |
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Frontiers Media S.A.
2021-04-01
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Series: | Frontiers in Immunology |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.645210/full |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pierre Rosenbaum Pierre Rosenbaum Nicolas Tchitchek Nicolas Tchitchek Candie Joly Candie Joly André Rodriguez Pozo André Rodriguez Pozo Lev Stimmer Lev Stimmer Sébastien Langlois Sébastien Langlois Hakim Hocini Hakim Hocini Leslie Gosse Leslie Gosse David Pejoski David Pejoski Antonio Cosma Antonio Cosma Anne-Sophie Beignon Anne-Sophie Beignon Nathalie Dereuddre-Bosquet Nathalie Dereuddre-Bosquet Yves Levy Yves Levy Roger Le Grand Roger Le Grand Frédéric Martinon Frédéric Martinon |
spellingShingle |
Pierre Rosenbaum Pierre Rosenbaum Nicolas Tchitchek Nicolas Tchitchek Candie Joly Candie Joly André Rodriguez Pozo André Rodriguez Pozo Lev Stimmer Lev Stimmer Sébastien Langlois Sébastien Langlois Hakim Hocini Hakim Hocini Leslie Gosse Leslie Gosse David Pejoski David Pejoski Antonio Cosma Antonio Cosma Anne-Sophie Beignon Anne-Sophie Beignon Nathalie Dereuddre-Bosquet Nathalie Dereuddre-Bosquet Yves Levy Yves Levy Roger Le Grand Roger Le Grand Frédéric Martinon Frédéric Martinon Vaccine Inoculation Route Modulates Early Immunity and Consequently Antigen-Specific Immune Response Frontiers in Immunology vaccine innate & adaptive immune response modified vaccinia Ankara (MVA) non human primates administration routes mass cytometry (CyTOF) |
author_facet |
Pierre Rosenbaum Pierre Rosenbaum Nicolas Tchitchek Nicolas Tchitchek Candie Joly Candie Joly André Rodriguez Pozo André Rodriguez Pozo Lev Stimmer Lev Stimmer Sébastien Langlois Sébastien Langlois Hakim Hocini Hakim Hocini Leslie Gosse Leslie Gosse David Pejoski David Pejoski Antonio Cosma Antonio Cosma Anne-Sophie Beignon Anne-Sophie Beignon Nathalie Dereuddre-Bosquet Nathalie Dereuddre-Bosquet Yves Levy Yves Levy Roger Le Grand Roger Le Grand Frédéric Martinon Frédéric Martinon |
author_sort |
Pierre Rosenbaum |
title |
Vaccine Inoculation Route Modulates Early Immunity and Consequently Antigen-Specific Immune Response |
title_short |
Vaccine Inoculation Route Modulates Early Immunity and Consequently Antigen-Specific Immune Response |
title_full |
Vaccine Inoculation Route Modulates Early Immunity and Consequently Antigen-Specific Immune Response |
title_fullStr |
Vaccine Inoculation Route Modulates Early Immunity and Consequently Antigen-Specific Immune Response |
title_full_unstemmed |
Vaccine Inoculation Route Modulates Early Immunity and Consequently Antigen-Specific Immune Response |
title_sort |
vaccine inoculation route modulates early immunity and consequently antigen-specific immune response |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2021-04-01 |
description |
Vaccination is one of the most efficient public healthcare measures to fight infectious diseases. Nevertheless, the immune mechanisms induced in vivo by vaccination are still unclear. The route of administration, an important vaccination parameter, can substantially modify the quality of the response. How the route of administration affects the generation and profile of immune responses is of major interest. Here, we aimed to extensively characterize the profiles of the innate and adaptive response to vaccination induced after intradermal, subcutaneous, or intramuscular administration with a modified vaccinia virus Ankara model vaccine in non-human primates. The adaptive response following subcutaneous immunization was clearly different from that following intradermal or intramuscular immunization. The subcutaneous route induced a higher level of neutralizing antibodies than the intradermal and intramuscular vaccination routes. In contrast, polyfunctional CD8+ T-cell responses were preferentially induced after intradermal or intramuscular injection. We observed the same dichotomy when analyzing the early molecular and cellular immune events, highlighting the recruitment of cell populations, such as CD8+ T lymphocytes and myeloid-derived suppressive cells, and the activation of key immunomodulatory gene pathways. These results demonstrate that the quality of the vaccine response induced by an attenuated vaccine is shaped by early and subtle modifications of the innate immune response. In this immunization context, the route of administration must be tailored to the desired type of protective immune response. This will be achieved through systems vaccinology and mathematical modeling, which will be critical for predicting the efficacy of the vaccination route for personalized medicine. |
topic |
vaccine innate & adaptive immune response modified vaccinia Ankara (MVA) non human primates administration routes mass cytometry (CyTOF) |
url |
https://www.frontiersin.org/articles/10.3389/fimmu.2021.645210/full |
work_keys_str_mv |
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doaj-3a103ecb80fc4060981474719f13c5bd2021-04-20T04:54:52ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-04-011210.3389/fimmu.2021.645210645210Vaccine Inoculation Route Modulates Early Immunity and Consequently Antigen-Specific Immune ResponsePierre Rosenbaum0Pierre Rosenbaum1Nicolas Tchitchek2Nicolas Tchitchek3Candie Joly4Candie Joly5André Rodriguez Pozo6André Rodriguez Pozo7Lev Stimmer8Lev Stimmer9Sébastien Langlois10Sébastien Langlois11Hakim Hocini12Hakim Hocini13Leslie Gosse14Leslie Gosse15David Pejoski16David Pejoski17Antonio Cosma18Antonio Cosma19Anne-Sophie Beignon20Anne-Sophie Beignon21Nathalie Dereuddre-Bosquet22Nathalie Dereuddre-Bosquet23Yves Levy24Yves Levy25Roger Le Grand26Roger Le Grand27Frédéric Martinon28Frédéric Martinon29UMR1184 IMVA-HB, IDMIT Department, Université Paris-Saclay – INSERM U1184 – CEA, Fontenay-aux-Roses, FranceVaccine Research Institute, Henri Mondor Hospital, Créteil, FranceUMR1184 IMVA-HB, IDMIT Department, Université Paris-Saclay – INSERM U1184 – CEA, Fontenay-aux-Roses, FranceVaccine Research Institute, Henri Mondor Hospital, Créteil, FranceUMR1184 IMVA-HB, IDMIT Department, Université Paris-Saclay – INSERM U1184 – CEA, Fontenay-aux-Roses, FranceVaccine Research Institute, Henri Mondor Hospital, Créteil, FranceUMR1184 IMVA-HB, IDMIT Department, Université Paris-Saclay – INSERM U1184 – CEA, Fontenay-aux-Roses, FranceVaccine Research Institute, Henri Mondor Hospital, Créteil, FranceINSERM, U1169, Kremlin-Bicêtre, FranceCEA – INSERM, MIRCen, UMS27, Fontenay-aux-Roses, FranceUMR1184 IMVA-HB, IDMIT Department, Université Paris-Saclay – INSERM U1184 – CEA, Fontenay-aux-Roses, FranceVaccine Research Institute, Henri Mondor Hospital, Créteil, FranceVaccine Research Institute, Henri Mondor Hospital, Créteil, FranceINSERM, U955, Team 16, Clinical and Infectious Diseases Department, Hospital Henri Mondor, University of Paris East, Créteil, FranceUMR1184 IMVA-HB, IDMIT Department, Université Paris-Saclay – INSERM U1184 – CEA, Fontenay-aux-Roses, FranceVaccine Research Institute, Henri Mondor Hospital, Créteil, FranceUMR1184 IMVA-HB, IDMIT Department, Université Paris-Saclay – INSERM U1184 – CEA, Fontenay-aux-Roses, FranceVaccine Research Institute, Henri Mondor Hospital, Créteil, FranceUMR1184 IMVA-HB, IDMIT Department, Université Paris-Saclay – INSERM U1184 – CEA, Fontenay-aux-Roses, FranceVaccine Research Institute, Henri Mondor Hospital, Créteil, FranceUMR1184 IMVA-HB, IDMIT Department, Université Paris-Saclay – INSERM U1184 – CEA, Fontenay-aux-Roses, FranceVaccine Research Institute, Henri Mondor Hospital, Créteil, FranceUMR1184 IMVA-HB, IDMIT Department, Université Paris-Saclay – INSERM U1184 – CEA, Fontenay-aux-Roses, FranceVaccine Research Institute, Henri Mondor Hospital, Créteil, FranceVaccine Research Institute, Henri Mondor Hospital, Créteil, FranceINSERM, U955, Team 16, Clinical and Infectious Diseases Department, Hospital Henri Mondor, University of Paris East, Créteil, FranceUMR1184 IMVA-HB, IDMIT Department, Université Paris-Saclay – INSERM U1184 – CEA, Fontenay-aux-Roses, FranceVaccine Research Institute, Henri Mondor Hospital, Créteil, FranceUMR1184 IMVA-HB, IDMIT Department, Université Paris-Saclay – INSERM U1184 – CEA, Fontenay-aux-Roses, FranceVaccine Research Institute, Henri Mondor Hospital, Créteil, FranceVaccination is one of the most efficient public healthcare measures to fight infectious diseases. Nevertheless, the immune mechanisms induced in vivo by vaccination are still unclear. The route of administration, an important vaccination parameter, can substantially modify the quality of the response. How the route of administration affects the generation and profile of immune responses is of major interest. Here, we aimed to extensively characterize the profiles of the innate and adaptive response to vaccination induced after intradermal, subcutaneous, or intramuscular administration with a modified vaccinia virus Ankara model vaccine in non-human primates. The adaptive response following subcutaneous immunization was clearly different from that following intradermal or intramuscular immunization. The subcutaneous route induced a higher level of neutralizing antibodies than the intradermal and intramuscular vaccination routes. In contrast, polyfunctional CD8+ T-cell responses were preferentially induced after intradermal or intramuscular injection. We observed the same dichotomy when analyzing the early molecular and cellular immune events, highlighting the recruitment of cell populations, such as CD8+ T lymphocytes and myeloid-derived suppressive cells, and the activation of key immunomodulatory gene pathways. These results demonstrate that the quality of the vaccine response induced by an attenuated vaccine is shaped by early and subtle modifications of the innate immune response. In this immunization context, the route of administration must be tailored to the desired type of protective immune response. This will be achieved through systems vaccinology and mathematical modeling, which will be critical for predicting the efficacy of the vaccination route for personalized medicine.https://www.frontiersin.org/articles/10.3389/fimmu.2021.645210/fullvaccineinnate & adaptive immune responsemodified vaccinia Ankara (MVA)non human primatesadministration routesmass cytometry (CyTOF) |