Characterization of inflammatory markers associated with systemic lupus erythematosus patients undergoing treatment
ABSTRACT Objective: To characterize the inflammatory profiles of patients with systemic lupus erythematosus receiving standard treatment compared to healthy controls. Patients and methods: Peripheral venous blood was collected from systemic lupus erythematosus patients (n = 14) and controls (n = 1...
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doaj-3a1e9eaa39174b0eb7548a9469cffb612020-11-24T21:53:26ZengSociedade Brasileira de Reumatologia Revista Brasileira de Reumatologia1809-457056649750310.1016/j.rbre.2016.02.009S0482-50042016000600497Characterization of inflammatory markers associated with systemic lupus erythematosus patients undergoing treatmentRodolfo Pessato TimóteoDouglas Cobo MicheliReginaldo Botelho TeodoroMarlene FreireDernival BertoncelloEddie Fernando Candido MurtaBeatriz Martins Tavares-MurtaABSTRACT Objective: To characterize the inflammatory profiles of patients with systemic lupus erythematosus receiving standard treatment compared to healthy controls. Patients and methods: Peripheral venous blood was collected from systemic lupus erythematosus patients (n = 14) and controls (n = 18) at enrollment. Blood samples were used for quantification, by flow cytometry, of CD11b (integrin) and Chemokine receptor CXCR2 expression surface antigen in neutrophils and lymphocytes, while cytokines were assayed in serum samples. Purified neutrophils were assayed by their ability to phagocytize human plasma-opsonized zymosan. Results: Patients had a median (interquartile range) disease activity index of 1.0 (0-2.0) characteristic of patients in remission. Interleukin-6 and interleukin-10 serum concentrations were significantly higher in the patient group compared to controls and the phagocytic index of circulating neutrophils was significantly reduced in patients compared to controls. The levels of interleukin-2, interleukin-5, interleukin-8 and tumor necrosis factor alpha did not significantly differ between patients and controls. Flow cytometric analysis revealed that the integrin expression levels were reduced in lymphocytes (but not in neutrophils) obtained from systemic lupus erythematosus patients, while surface expression of the chemokine receptor 2 was similar in both neutrophils and lymphocytes. Conclusion: Systemic lupus erythematosus patients receiving standard treatment presented with elevated systemic levels of interleukin-6 and interleukin-10, reduced neutrophil phagocytic capacity, and reduced lymphocyte expression of integrin even when symptoms were in remission. These alterations to innate immune components may put these individuals at a greater risk for acquiring infections.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0482-50042016000600497&lng=en&tlng=enLúpus eritematoso sistêmicoCitocinasFagocitoseIntegrinaCXCR2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rodolfo Pessato Timóteo Douglas Cobo Micheli Reginaldo Botelho Teodoro Marlene Freire Dernival Bertoncello Eddie Fernando Candido Murta Beatriz Martins Tavares-Murta |
spellingShingle |
Rodolfo Pessato Timóteo Douglas Cobo Micheli Reginaldo Botelho Teodoro Marlene Freire Dernival Bertoncello Eddie Fernando Candido Murta Beatriz Martins Tavares-Murta Characterization of inflammatory markers associated with systemic lupus erythematosus patients undergoing treatment Revista Brasileira de Reumatologia Lúpus eritematoso sistêmico Citocinas Fagocitose Integrina CXCR2 |
author_facet |
Rodolfo Pessato Timóteo Douglas Cobo Micheli Reginaldo Botelho Teodoro Marlene Freire Dernival Bertoncello Eddie Fernando Candido Murta Beatriz Martins Tavares-Murta |
author_sort |
Rodolfo Pessato Timóteo |
title |
Characterization of inflammatory markers associated with systemic lupus erythematosus patients undergoing treatment |
title_short |
Characterization of inflammatory markers associated with systemic lupus erythematosus patients undergoing treatment |
title_full |
Characterization of inflammatory markers associated with systemic lupus erythematosus patients undergoing treatment |
title_fullStr |
Characterization of inflammatory markers associated with systemic lupus erythematosus patients undergoing treatment |
title_full_unstemmed |
Characterization of inflammatory markers associated with systemic lupus erythematosus patients undergoing treatment |
title_sort |
characterization of inflammatory markers associated with systemic lupus erythematosus patients undergoing treatment |
publisher |
Sociedade Brasileira de Reumatologia |
series |
Revista Brasileira de Reumatologia |
issn |
1809-4570 |
description |
ABSTRACT Objective: To characterize the inflammatory profiles of patients with systemic lupus erythematosus receiving standard treatment compared to healthy controls. Patients and methods: Peripheral venous blood was collected from systemic lupus erythematosus patients (n = 14) and controls (n = 18) at enrollment. Blood samples were used for quantification, by flow cytometry, of CD11b (integrin) and Chemokine receptor CXCR2 expression surface antigen in neutrophils and lymphocytes, while cytokines were assayed in serum samples. Purified neutrophils were assayed by their ability to phagocytize human plasma-opsonized zymosan. Results: Patients had a median (interquartile range) disease activity index of 1.0 (0-2.0) characteristic of patients in remission. Interleukin-6 and interleukin-10 serum concentrations were significantly higher in the patient group compared to controls and the phagocytic index of circulating neutrophils was significantly reduced in patients compared to controls. The levels of interleukin-2, interleukin-5, interleukin-8 and tumor necrosis factor alpha did not significantly differ between patients and controls. Flow cytometric analysis revealed that the integrin expression levels were reduced in lymphocytes (but not in neutrophils) obtained from systemic lupus erythematosus patients, while surface expression of the chemokine receptor 2 was similar in both neutrophils and lymphocytes. Conclusion: Systemic lupus erythematosus patients receiving standard treatment presented with elevated systemic levels of interleukin-6 and interleukin-10, reduced neutrophil phagocytic capacity, and reduced lymphocyte expression of integrin even when symptoms were in remission. These alterations to innate immune components may put these individuals at a greater risk for acquiring infections. |
topic |
Lúpus eritematoso sistêmico Citocinas Fagocitose Integrina CXCR2 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0482-50042016000600497&lng=en&tlng=en |
work_keys_str_mv |
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