DLX5 and HOXC8 enhance the chondrogenic differentiation potential of stem cells from apical papilla via LINC01013
Abstract Background Mesenchymal stem cell (MSC)-based cartilage tissue regeneration is a treatment with great potential. How to enhance the MSC chondrogenic differentiation is a key issue involved in cartilage formation. In the present study, we seek to expound the phenotypes and mechanisms of DLX5...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2020-07-01
|
Series: | Stem Cell Research & Therapy |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13287-020-01791-8 |
id |
doaj-3a3134b4534149fa996c6104d1995479 |
---|---|
record_format |
Article |
spelling |
doaj-3a3134b4534149fa996c6104d19954792020-11-25T02:14:05ZengBMCStem Cell Research & Therapy1757-65122020-07-0111111610.1186/s13287-020-01791-8DLX5 and HOXC8 enhance the chondrogenic differentiation potential of stem cells from apical papilla via LINC01013Haoqing Yang0Yangyang Cao1Jianpeng Zhang2Yuncun Liang3Xiaomin Su4Chen Zhang5Huina Liu6Xiao Han7Lihua Ge8Zhipeng Fan9Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatology Hospital, School of Stomatology, Capital Medical UniversityBeijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatology Hospital, School of Stomatology, Capital Medical UniversityDepartment of Endodontics, Beijing Stomatological Hospital, School of Stomatology, Capital Medical UniversityBeijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatology Hospital, School of Stomatology, Capital Medical UniversityBeijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatology Hospital, School of Stomatology, Capital Medical UniversityBeijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatology Hospital, School of Stomatology, Capital Medical UniversityBeijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatology Hospital, School of Stomatology, Capital Medical UniversityBeijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatology Hospital, School of Stomatology, Capital Medical UniversityBeijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatology Hospital, School of Stomatology, Capital Medical UniversityBeijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatology Hospital, School of Stomatology, Capital Medical UniversityAbstract Background Mesenchymal stem cell (MSC)-based cartilage tissue regeneration is a treatment with great potential. How to enhance the MSC chondrogenic differentiation is a key issue involved in cartilage formation. In the present study, we seek to expound the phenotypes and mechanisms of DLX5 in chondrogenic differentiation function in MSCs. Methods Stem cells from apical papilla (SCAPs) were used. The Alcian Blue staining, pellet culture system, and cell transplantation in rabbit knee cartilage defect were used to evaluate the chondrogenic differentiation function of MSCs. Western blot, real-time RT-PCR, and ChIP assays were used to evaluate the molecular mechanisms. Results DLX5 and HOXC8 expressions were upregulated during chondrogenic differentiation. In vitro results showed that DLX5 and HOXC8 enhanced the expression of chondrogenic markers including collagen II (COL2), collagen V (COL5), and sex-determining region Y box protein 9 (SOX9) and promoted the chondrogenic differentiation and the formation of cartilage clumps in the pellet culture system. Mechanically, DLX5 and HOXC8 formed protein complexes and negatively regulated the LncRNA, LINC01013, via directly binding its promoter. In vivo transplantation experiment showed that DLX5 and HOXC8 could restore the cartilage defect in the rabbit knee model. In addition, knock-down of LINC01013 enhanced the chondrogenic differentiation of SCAPs. Conclusions In conclusion, DLX5 and HOXC8 enhance the chondrogenic differentiation abilities of SCAPs by negatively regulating LINC01013 in SCAPs, and provided the potential target for promoting cartilage tissue regeneration.http://link.springer.com/article/10.1186/s13287-020-01791-8DLX5HOXC8Chondrogenic differentiationStem cells from apical papilla (SCAPs)LncRNA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Haoqing Yang Yangyang Cao Jianpeng Zhang Yuncun Liang Xiaomin Su Chen Zhang Huina Liu Xiao Han Lihua Ge Zhipeng Fan |
spellingShingle |
Haoqing Yang Yangyang Cao Jianpeng Zhang Yuncun Liang Xiaomin Su Chen Zhang Huina Liu Xiao Han Lihua Ge Zhipeng Fan DLX5 and HOXC8 enhance the chondrogenic differentiation potential of stem cells from apical papilla via LINC01013 Stem Cell Research & Therapy DLX5 HOXC8 Chondrogenic differentiation Stem cells from apical papilla (SCAPs) LncRNA |
author_facet |
Haoqing Yang Yangyang Cao Jianpeng Zhang Yuncun Liang Xiaomin Su Chen Zhang Huina Liu Xiao Han Lihua Ge Zhipeng Fan |
author_sort |
Haoqing Yang |
title |
DLX5 and HOXC8 enhance the chondrogenic differentiation potential of stem cells from apical papilla via LINC01013 |
title_short |
DLX5 and HOXC8 enhance the chondrogenic differentiation potential of stem cells from apical papilla via LINC01013 |
title_full |
DLX5 and HOXC8 enhance the chondrogenic differentiation potential of stem cells from apical papilla via LINC01013 |
title_fullStr |
DLX5 and HOXC8 enhance the chondrogenic differentiation potential of stem cells from apical papilla via LINC01013 |
title_full_unstemmed |
DLX5 and HOXC8 enhance the chondrogenic differentiation potential of stem cells from apical papilla via LINC01013 |
title_sort |
dlx5 and hoxc8 enhance the chondrogenic differentiation potential of stem cells from apical papilla via linc01013 |
publisher |
BMC |
series |
Stem Cell Research & Therapy |
issn |
1757-6512 |
publishDate |
2020-07-01 |
description |
Abstract Background Mesenchymal stem cell (MSC)-based cartilage tissue regeneration is a treatment with great potential. How to enhance the MSC chondrogenic differentiation is a key issue involved in cartilage formation. In the present study, we seek to expound the phenotypes and mechanisms of DLX5 in chondrogenic differentiation function in MSCs. Methods Stem cells from apical papilla (SCAPs) were used. The Alcian Blue staining, pellet culture system, and cell transplantation in rabbit knee cartilage defect were used to evaluate the chondrogenic differentiation function of MSCs. Western blot, real-time RT-PCR, and ChIP assays were used to evaluate the molecular mechanisms. Results DLX5 and HOXC8 expressions were upregulated during chondrogenic differentiation. In vitro results showed that DLX5 and HOXC8 enhanced the expression of chondrogenic markers including collagen II (COL2), collagen V (COL5), and sex-determining region Y box protein 9 (SOX9) and promoted the chondrogenic differentiation and the formation of cartilage clumps in the pellet culture system. Mechanically, DLX5 and HOXC8 formed protein complexes and negatively regulated the LncRNA, LINC01013, via directly binding its promoter. In vivo transplantation experiment showed that DLX5 and HOXC8 could restore the cartilage defect in the rabbit knee model. In addition, knock-down of LINC01013 enhanced the chondrogenic differentiation of SCAPs. Conclusions In conclusion, DLX5 and HOXC8 enhance the chondrogenic differentiation abilities of SCAPs by negatively regulating LINC01013 in SCAPs, and provided the potential target for promoting cartilage tissue regeneration. |
topic |
DLX5 HOXC8 Chondrogenic differentiation Stem cells from apical papilla (SCAPs) LncRNA |
url |
http://link.springer.com/article/10.1186/s13287-020-01791-8 |
work_keys_str_mv |
AT haoqingyang dlx5andhoxc8enhancethechondrogenicdifferentiationpotentialofstemcellsfromapicalpapillavialinc01013 AT yangyangcao dlx5andhoxc8enhancethechondrogenicdifferentiationpotentialofstemcellsfromapicalpapillavialinc01013 AT jianpengzhang dlx5andhoxc8enhancethechondrogenicdifferentiationpotentialofstemcellsfromapicalpapillavialinc01013 AT yuncunliang dlx5andhoxc8enhancethechondrogenicdifferentiationpotentialofstemcellsfromapicalpapillavialinc01013 AT xiaominsu dlx5andhoxc8enhancethechondrogenicdifferentiationpotentialofstemcellsfromapicalpapillavialinc01013 AT chenzhang dlx5andhoxc8enhancethechondrogenicdifferentiationpotentialofstemcellsfromapicalpapillavialinc01013 AT huinaliu dlx5andhoxc8enhancethechondrogenicdifferentiationpotentialofstemcellsfromapicalpapillavialinc01013 AT xiaohan dlx5andhoxc8enhancethechondrogenicdifferentiationpotentialofstemcellsfromapicalpapillavialinc01013 AT lihuage dlx5andhoxc8enhancethechondrogenicdifferentiationpotentialofstemcellsfromapicalpapillavialinc01013 AT zhipengfan dlx5andhoxc8enhancethechondrogenicdifferentiationpotentialofstemcellsfromapicalpapillavialinc01013 |
_version_ |
1724902071307599872 |