Natural Interactions between S. haematobium and S. guineensis in the Republic of Benin
Schistosomiasis is a parasitic disease which affects millions of people around the world, particularly in Africa. In this continent, different species are able to interbreed, like Schistosoma haematobium and Schistosoma guineensis, two schistosome species infecting humans. The Republic of Benin is k...
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doaj-3a35272a66bc4571a5553b2eac985f432020-11-25T00:16:48ZengHindawi LimitedThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/793420793420Natural Interactions between S. haematobium and S. guineensis in the Republic of BeninHélène Moné0Stéphanie Minguez1Moudachirou Ibikounlé2Jean-François Allienne3Achille Massougbodji4Gabriel Mouahid5Université de Perpignan Via Domitia, Ecologie et Evolution des Interactions, UMR 5244, 66860 Perpignan, FranceUniversité de Perpignan Via Domitia, Ecologie et Evolution des Interactions, UMR 5244, 66860 Perpignan, FranceDépartement de Zoologie et Génétique, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 01BP526 Cotonou, BeninUniversité de Perpignan Via Domitia, Ecologie et Evolution des Interactions, UMR 5244, 66860 Perpignan, FranceLaboratoire de Parasitologie-Mycologie, Faculté des Sciences de la Santé, Université d’Abomey-Calavi, 01BP188 Cotonou, BeninUniversité de Perpignan Via Domitia, Ecologie et Evolution des Interactions, UMR 5244, 66860 Perpignan, FranceSchistosomiasis is a parasitic disease which affects millions of people around the world, particularly in Africa. In this continent, different species are able to interbreed, like Schistosoma haematobium and Schistosoma guineensis, two schistosome species infecting humans. The Republic of Benin is known to harbor S. haematobium, but its geographical situation in between Nigeria, Mali, and Burkina Faso, where S. guineensis was found, raises the question about the possible presence of S. haematobium/S. guineensis hybrids in this country. We conducted morphological analyses on schistosome eggs and molecular analyses on schistosome larvae (high resolution melting (HRM) analysis and gene sequencing) in order to detect any natural interaction between these two species of schistosomes. The morphological results showed the presence of three egg morphotypes (S. haematobium, S. guineensis, and intermediate). Three genotypes were detected by ITS2 rDNA HRM analysis: S. haematobium, S. guineensis, and hybrid, and their percentages confirmed the results of the morphological analysis. However, sequencing of the CO1 mtDNA gene showed that all the samples from Benin belonged to S. haematobium. Our results provide the first evidence of introgression of S. guineensis genes in S. haematobium in Benin.http://dx.doi.org/10.1100/2012/793420 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hélène Moné Stéphanie Minguez Moudachirou Ibikounlé Jean-François Allienne Achille Massougbodji Gabriel Mouahid |
spellingShingle |
Hélène Moné Stéphanie Minguez Moudachirou Ibikounlé Jean-François Allienne Achille Massougbodji Gabriel Mouahid Natural Interactions between S. haematobium and S. guineensis in the Republic of Benin The Scientific World Journal |
author_facet |
Hélène Moné Stéphanie Minguez Moudachirou Ibikounlé Jean-François Allienne Achille Massougbodji Gabriel Mouahid |
author_sort |
Hélène Moné |
title |
Natural Interactions between S. haematobium and S. guineensis in the Republic of Benin |
title_short |
Natural Interactions between S. haematobium and S. guineensis in the Republic of Benin |
title_full |
Natural Interactions between S. haematobium and S. guineensis in the Republic of Benin |
title_fullStr |
Natural Interactions between S. haematobium and S. guineensis in the Republic of Benin |
title_full_unstemmed |
Natural Interactions between S. haematobium and S. guineensis in the Republic of Benin |
title_sort |
natural interactions between s. haematobium and s. guineensis in the republic of benin |
publisher |
Hindawi Limited |
series |
The Scientific World Journal |
issn |
1537-744X |
publishDate |
2012-01-01 |
description |
Schistosomiasis is a parasitic disease which affects millions of people around the world, particularly in Africa. In this continent, different species are able to interbreed, like Schistosoma haematobium and Schistosoma guineensis, two schistosome species infecting humans. The Republic of Benin is known to harbor S. haematobium, but its geographical situation in between Nigeria, Mali, and Burkina Faso, where S. guineensis was found, raises the question about the possible presence of S. haematobium/S. guineensis hybrids in this country. We conducted morphological analyses on schistosome eggs and molecular analyses on schistosome larvae (high resolution melting (HRM) analysis and gene sequencing) in order to detect any natural interaction between these two species of schistosomes. The morphological results showed the presence of three egg morphotypes (S. haematobium, S. guineensis, and intermediate). Three genotypes were detected by ITS2 rDNA HRM analysis: S. haematobium, S. guineensis, and hybrid, and their percentages confirmed the results of the morphological analysis. However, sequencing of the CO1 mtDNA gene showed that all the samples from Benin belonged to S. haematobium. Our results provide the first evidence of introgression of S. guineensis genes in S. haematobium in Benin. |
url |
http://dx.doi.org/10.1100/2012/793420 |
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