Inactivation of <i>Acanthamoeba</i> Cysts in Suspension and on Contaminated Contact Lenses Using Non-Thermal Plasma
Water suspensions of cysts of a pathogenic clinical isolate of <i>Acanthamoeba</i> sp. were prepared, and the cysts were inactivated either in suspension or placed on the surface of contact lenses by the non-thermal plasma produced by the DC corona transient spark discharge. The efficacy...
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MDPI AG
2021-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/9/1879 |
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doaj-3a3864e1164b4851b691ed309028d05b2021-09-26T00:43:37ZengMDPI AGMicroorganisms2076-26072021-09-0191879187910.3390/microorganisms9091879Inactivation of <i>Acanthamoeba</i> Cysts in Suspension and on Contaminated Contact Lenses Using Non-Thermal PlasmaTereza Měřínská0Vladimír Scholtz1Josef Khun2Jaroslav Julák3Eva Nohýnková4Department of Physics and Measurements, University of Chemistry and Technology, 166 28 Prague, Czech RepublicDepartment of Physics and Measurements, University of Chemistry and Technology, 166 28 Prague, Czech RepublicDepartment of Physics and Measurements, University of Chemistry and Technology, 166 28 Prague, Czech RepublicInstitute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University and General University Hospital in Prague, 128 00 Prague, Czech RepublicInstitute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University and General University Hospital in Prague, 128 00 Prague, Czech RepublicWater suspensions of cysts of a pathogenic clinical isolate of <i>Acanthamoeba</i> sp. were prepared, and the cysts were inactivated either in suspension or placed on the surface of contact lenses by the non-thermal plasma produced by the DC corona transient spark discharge. The efficacy of this treatment was determined by cultivation and the presence of vegetative trophozoites indicating non-inactivated cysts. The negative discharge appeared to be more effective than the positive one. The complete inactivation occurred in water suspension after 40 min and on contaminated lenses after 50 min of plasma exposure. The properties of lenses seem to not be affected by plasma exposure; that is, their optical power, diameter, curvature, water content and infrared and Raman spectra remain unchanged.https://www.mdpi.com/2076-2607/9/9/1879<i>Acanthamoeba</i>cystscontact lensesDC corona dischargeIR spectraRaman spectra |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Tereza Měřínská Vladimír Scholtz Josef Khun Jaroslav Julák Eva Nohýnková |
| spellingShingle |
Tereza Měřínská Vladimír Scholtz Josef Khun Jaroslav Julák Eva Nohýnková Inactivation of <i>Acanthamoeba</i> Cysts in Suspension and on Contaminated Contact Lenses Using Non-Thermal Plasma Microorganisms <i>Acanthamoeba</i> cysts contact lenses DC corona discharge IR spectra Raman spectra |
| author_facet |
Tereza Měřínská Vladimír Scholtz Josef Khun Jaroslav Julák Eva Nohýnková |
| author_sort |
Tereza Měřínská |
| title |
Inactivation of <i>Acanthamoeba</i> Cysts in Suspension and on Contaminated Contact Lenses Using Non-Thermal Plasma |
| title_short |
Inactivation of <i>Acanthamoeba</i> Cysts in Suspension and on Contaminated Contact Lenses Using Non-Thermal Plasma |
| title_full |
Inactivation of <i>Acanthamoeba</i> Cysts in Suspension and on Contaminated Contact Lenses Using Non-Thermal Plasma |
| title_fullStr |
Inactivation of <i>Acanthamoeba</i> Cysts in Suspension and on Contaminated Contact Lenses Using Non-Thermal Plasma |
| title_full_unstemmed |
Inactivation of <i>Acanthamoeba</i> Cysts in Suspension and on Contaminated Contact Lenses Using Non-Thermal Plasma |
| title_sort |
inactivation of <i>acanthamoeba</i> cysts in suspension and on contaminated contact lenses using non-thermal plasma |
| publisher |
MDPI AG |
| series |
Microorganisms |
| issn |
2076-2607 |
| publishDate |
2021-09-01 |
| description |
Water suspensions of cysts of a pathogenic clinical isolate of <i>Acanthamoeba</i> sp. were prepared, and the cysts were inactivated either in suspension or placed on the surface of contact lenses by the non-thermal plasma produced by the DC corona transient spark discharge. The efficacy of this treatment was determined by cultivation and the presence of vegetative trophozoites indicating non-inactivated cysts. The negative discharge appeared to be more effective than the positive one. The complete inactivation occurred in water suspension after 40 min and on contaminated lenses after 50 min of plasma exposure. The properties of lenses seem to not be affected by plasma exposure; that is, their optical power, diameter, curvature, water content and infrared and Raman spectra remain unchanged. |
| topic |
<i>Acanthamoeba</i> cysts contact lenses DC corona discharge IR spectra Raman spectra |
| url |
https://www.mdpi.com/2076-2607/9/9/1879 |
| work_keys_str_mv |
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