Alcohol-soluble polysaccharide from Castanea mollissima blume: Preparation, characteristics and antitumor activity
CMBP was an alcohol-soluble polysaccharide extracted from Castanea mollissima blume. Our study aimed to investigate the preliminary structural characteristics and in vivo antitumor activity of CMBP. The contents of total sugar, protein and uronic acid in CMBP was 93.68%, 0.89% and 1.78%, respectivel...
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doaj-3a3bc0d74a3045b68c16efe6c2d000f62021-04-30T07:17:23ZengElsevierJournal of Functional Foods1756-46462019-12-0163Alcohol-soluble polysaccharide from Castanea mollissima blume: Preparation, characteristics and antitumor activityXiao-dan Dong0Juan Yu1Ying-ying Feng2Hai-yu Ji3Sha-Sha Yu4An-jun Liu5College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; QingYunTang Biotech (Beijing) Co., Ltd., No. 14, Zhonghe Street, Beijing Economic-Technological Development Area, Beijing 100176, ChinaCollege of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; Corresponding authors at: Tianjin Key Laboratory of Food Nutrition and Safety, School of Food Engineering and Biological Technology, Tianjin University of Science and Technology, No. 29, 13th Avenue, Tianjin Economic and Technological Development Zone, Tianjin 300457, China.College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; QingYunTang Biotech (Beijing) Co., Ltd., No. 14, Zhonghe Street, Beijing Economic-Technological Development Area, Beijing 100176, ChinaCollege of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; QingYunTang Biotech (Beijing) Co., Ltd., No. 14, Zhonghe Street, Beijing Economic-Technological Development Area, Beijing 100176, ChinaCollege of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; QingYunTang Biotech (Beijing) Co., Ltd., No. 14, Zhonghe Street, Beijing Economic-Technological Development Area, Beijing 100176, ChinaCollege of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; Corresponding authors at: Tianjin Key Laboratory of Food Nutrition and Safety, School of Food Engineering and Biological Technology, Tianjin University of Science and Technology, No. 29, 13th Avenue, Tianjin Economic and Technological Development Zone, Tianjin 300457, China.CMBP was an alcohol-soluble polysaccharide extracted from Castanea mollissima blume. Our study aimed to investigate the preliminary structural characteristics and in vivo antitumor activity of CMBP. The contents of total sugar, protein and uronic acid in CMBP was 93.68%, 0.89% and 1.78%, respectively. GC and FTIR results suggested galactose and glucose were detected for CMBP (2.86 × 103 Da), while glucose was the dominant monosaccharide in CMBP and pyranose form and α- and β-type glycosidic linkages were founded for CMBP. Meanwhile, CMBP could exhibite strong antitumor effects on S180 tumor-bearing mice. The antitumor effects of CMBP were related to improving the immune response, which result from improving the levels of serum cytokines (TNF-α, IL-2 and IFN-γ), activating immune cells (macrophages, lymphocytes and NK cells), adjusting the distribution of lymphocyte subsets. The H&E staining and cell cycle results indicated CMBP could induce tumor cells apoptosis and extensive injuries.http://www.sciencedirect.com/science/article/pii/S1756464619304876Alcohol-soluble polysaccharideCastanea mollissima blumeCharacteristicsAntitumor immunity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiao-dan Dong Juan Yu Ying-ying Feng Hai-yu Ji Sha-Sha Yu An-jun Liu |
spellingShingle |
Xiao-dan Dong Juan Yu Ying-ying Feng Hai-yu Ji Sha-Sha Yu An-jun Liu Alcohol-soluble polysaccharide from Castanea mollissima blume: Preparation, characteristics and antitumor activity Journal of Functional Foods Alcohol-soluble polysaccharide Castanea mollissima blume Characteristics Antitumor immunity |
author_facet |
Xiao-dan Dong Juan Yu Ying-ying Feng Hai-yu Ji Sha-Sha Yu An-jun Liu |
author_sort |
Xiao-dan Dong |
title |
Alcohol-soluble polysaccharide from Castanea mollissima blume: Preparation, characteristics and antitumor activity |
title_short |
Alcohol-soluble polysaccharide from Castanea mollissima blume: Preparation, characteristics and antitumor activity |
title_full |
Alcohol-soluble polysaccharide from Castanea mollissima blume: Preparation, characteristics and antitumor activity |
title_fullStr |
Alcohol-soluble polysaccharide from Castanea mollissima blume: Preparation, characteristics and antitumor activity |
title_full_unstemmed |
Alcohol-soluble polysaccharide from Castanea mollissima blume: Preparation, characteristics and antitumor activity |
title_sort |
alcohol-soluble polysaccharide from castanea mollissima blume: preparation, characteristics and antitumor activity |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2019-12-01 |
description |
CMBP was an alcohol-soluble polysaccharide extracted from Castanea mollissima blume. Our study aimed to investigate the preliminary structural characteristics and in vivo antitumor activity of CMBP. The contents of total sugar, protein and uronic acid in CMBP was 93.68%, 0.89% and 1.78%, respectively. GC and FTIR results suggested galactose and glucose were detected for CMBP (2.86 × 103 Da), while glucose was the dominant monosaccharide in CMBP and pyranose form and α- and β-type glycosidic linkages were founded for CMBP. Meanwhile, CMBP could exhibite strong antitumor effects on S180 tumor-bearing mice. The antitumor effects of CMBP were related to improving the immune response, which result from improving the levels of serum cytokines (TNF-α, IL-2 and IFN-γ), activating immune cells (macrophages, lymphocytes and NK cells), adjusting the distribution of lymphocyte subsets. The H&E staining and cell cycle results indicated CMBP could induce tumor cells apoptosis and extensive injuries. |
topic |
Alcohol-soluble polysaccharide Castanea mollissima blume Characteristics Antitumor immunity |
url |
http://www.sciencedirect.com/science/article/pii/S1756464619304876 |
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