Oleuropein Transcriptionally Primes Lactobacillus plantarum to Interact With Plant Hosts
Oleuropein (OLE) is a secoiridoid unique to Oleaceae known to play a role in the plant–herbivore interaction. However, it is not clear how this molecule is induced to mediate plant responses to microbes and how microbes, in turn, withstand with OLE. To better understand how OLE affects the plant–mic...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2019-09-01
|
Series: | Frontiers in Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02177/full |
id |
doaj-3a415951f974480495d3dbe4b3c0d47b |
---|---|
record_format |
Article |
spelling |
doaj-3a415951f974480495d3dbe4b3c0d47b2020-11-25T02:18:33ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-09-011010.3389/fmicb.2019.02177457321Oleuropein Transcriptionally Primes Lactobacillus plantarum to Interact With Plant HostsLaura Santamaría0Inés Reverón1Laura Plaza-Vinuesa2Juan Carlos Oliveros3Blanca de las Rivas4Rosario Muñoz5Félix López de Felipe6Laboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de los Alimentos y Nutrición (ICTAN-CSIC), Madrid, SpainLaboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de los Alimentos y Nutrición (ICTAN-CSIC), Madrid, SpainLaboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de los Alimentos y Nutrición (ICTAN-CSIC), Madrid, SpainNational Center for Biotechnology (CNB-CSIC), Madrid, SpainLaboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de los Alimentos y Nutrición (ICTAN-CSIC), Madrid, SpainLaboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de los Alimentos y Nutrición (ICTAN-CSIC), Madrid, SpainLaboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de los Alimentos y Nutrición (ICTAN-CSIC), Madrid, SpainOleuropein (OLE) is a secoiridoid unique to Oleaceae known to play a role in the plant–herbivore interaction. However, it is not clear how this molecule is induced to mediate plant responses to microbes and how microbes, in turn, withstand with OLE. To better understand how OLE affects the plant–microbe interaction, the contribution of differential gene expression in the adaptation to OLE was characterized by whole genome transcriptional profiling in Lactobacillus plantarum, a bacterium associated to the olive. OLE downregulated functions associated to rapid growth, remodeled membrane phospholipid biosynthesis pathways and markedly repressed the expression of several ABC transporters from L. plantarum. Genes encoding the plantaricin and lamABDCA quorum-sensing (QS) systems were down-regulated indicating the potential of OLE as a QS-antagonist. Notably, OLE diminished the expression of a set of genes encoding inmunomodulatory components and reoriented metabolic pathways to increase protein acetylation, probably to attenuate plant immunity. Responses were also triggered to repress the transport of acetoin and to buffer reactive oxygen species accumulation, two signals involved in plant development. The results suggest that OLE could act as a signaling molecule in the plant–microbe interaction and facilitate the accommodation of beneficial microbes such as L. plantarum by the plant host, via controlled expression of bacterial molecular players involved in this reciprocal interplay.https://www.frontiersin.org/article/10.3389/fmicb.2019.02177/fulloleuropeintranscriptomicsLactobacillus plantarumsignalingquorum sensinginmunomodulators |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Laura Santamaría Inés Reverón Laura Plaza-Vinuesa Juan Carlos Oliveros Blanca de las Rivas Rosario Muñoz Félix López de Felipe |
spellingShingle |
Laura Santamaría Inés Reverón Laura Plaza-Vinuesa Juan Carlos Oliveros Blanca de las Rivas Rosario Muñoz Félix López de Felipe Oleuropein Transcriptionally Primes Lactobacillus plantarum to Interact With Plant Hosts Frontiers in Microbiology oleuropein transcriptomics Lactobacillus plantarum signaling quorum sensing inmunomodulators |
author_facet |
Laura Santamaría Inés Reverón Laura Plaza-Vinuesa Juan Carlos Oliveros Blanca de las Rivas Rosario Muñoz Félix López de Felipe |
author_sort |
Laura Santamaría |
title |
Oleuropein Transcriptionally Primes Lactobacillus plantarum to Interact With Plant Hosts |
title_short |
Oleuropein Transcriptionally Primes Lactobacillus plantarum to Interact With Plant Hosts |
title_full |
Oleuropein Transcriptionally Primes Lactobacillus plantarum to Interact With Plant Hosts |
title_fullStr |
Oleuropein Transcriptionally Primes Lactobacillus plantarum to Interact With Plant Hosts |
title_full_unstemmed |
Oleuropein Transcriptionally Primes Lactobacillus plantarum to Interact With Plant Hosts |
title_sort |
oleuropein transcriptionally primes lactobacillus plantarum to interact with plant hosts |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2019-09-01 |
description |
Oleuropein (OLE) is a secoiridoid unique to Oleaceae known to play a role in the plant–herbivore interaction. However, it is not clear how this molecule is induced to mediate plant responses to microbes and how microbes, in turn, withstand with OLE. To better understand how OLE affects the plant–microbe interaction, the contribution of differential gene expression in the adaptation to OLE was characterized by whole genome transcriptional profiling in Lactobacillus plantarum, a bacterium associated to the olive. OLE downregulated functions associated to rapid growth, remodeled membrane phospholipid biosynthesis pathways and markedly repressed the expression of several ABC transporters from L. plantarum. Genes encoding the plantaricin and lamABDCA quorum-sensing (QS) systems were down-regulated indicating the potential of OLE as a QS-antagonist. Notably, OLE diminished the expression of a set of genes encoding inmunomodulatory components and reoriented metabolic pathways to increase protein acetylation, probably to attenuate plant immunity. Responses were also triggered to repress the transport of acetoin and to buffer reactive oxygen species accumulation, two signals involved in plant development. The results suggest that OLE could act as a signaling molecule in the plant–microbe interaction and facilitate the accommodation of beneficial microbes such as L. plantarum by the plant host, via controlled expression of bacterial molecular players involved in this reciprocal interplay. |
topic |
oleuropein transcriptomics Lactobacillus plantarum signaling quorum sensing inmunomodulators |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2019.02177/full |
work_keys_str_mv |
AT laurasantamaria oleuropeintranscriptionallyprimeslactobacillusplantarumtointeractwithplanthosts AT inesreveron oleuropeintranscriptionallyprimeslactobacillusplantarumtointeractwithplanthosts AT lauraplazavinuesa oleuropeintranscriptionallyprimeslactobacillusplantarumtointeractwithplanthosts AT juancarlosoliveros oleuropeintranscriptionallyprimeslactobacillusplantarumtointeractwithplanthosts AT blancadelasrivas oleuropeintranscriptionallyprimeslactobacillusplantarumtointeractwithplanthosts AT rosariomunoz oleuropeintranscriptionallyprimeslactobacillusplantarumtointeractwithplanthosts AT felixlopezdefelipe oleuropeintranscriptionallyprimeslactobacillusplantarumtointeractwithplanthosts |
_version_ |
1724881373367369728 |