Association of Pre-diagnostic Antibody Responses to Escherichia coli and Bacteroides fragilis Toxin Proteins with Colorectal Cancer in a European Cohort
Experimental evidence has implicated genotoxic Escherichia coli (E. coli) and enterotoxigenic Bacteroides fragilis (ETBF) in the development of colorectal cancer (CRC). However, evidence from epidemiological studies is sparse. We therefore assessed the association of serological markers of E. coli a...
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Language: | English |
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Taylor & Francis Group
2021-01-01
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Series: | Gut Microbes |
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Online Access: | http://dx.doi.org/10.1080/19490976.2021.1903825 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Julia Butt Mazda Jenab Jill Werner Veronika Fedirko Elisabete Weiderpass Christina C. Dahm Anne Tjønneland Anja Olsen Marie-Christine Boutron-Ruault Joseph A. Rothwell Gianluca Severi Rudolf Kaaks Renée Turzanski-Fortner Krasimira Aleksandrova Matthias Schulze Domenico Palli Valeria Pala Salvatore Panico Rosario Tumino Carlotta Sacerdote Bas Bueno-de-Mesquita Carla H. Van Gils Inger Torhild Gram Marko Lukic Núria Sala María José Sánchez Pérez Eva Ardanaz María-Dolores Chirlaque Richard Palmquist Thyra Löwenmark Ruth C Travis Alicia Heath Amanda J Cross Heinz Freisling Semi Zouiouich Elom Aglago Tim Waterboer David J. Hughes |
spellingShingle |
Julia Butt Mazda Jenab Jill Werner Veronika Fedirko Elisabete Weiderpass Christina C. Dahm Anne Tjønneland Anja Olsen Marie-Christine Boutron-Ruault Joseph A. Rothwell Gianluca Severi Rudolf Kaaks Renée Turzanski-Fortner Krasimira Aleksandrova Matthias Schulze Domenico Palli Valeria Pala Salvatore Panico Rosario Tumino Carlotta Sacerdote Bas Bueno-de-Mesquita Carla H. Van Gils Inger Torhild Gram Marko Lukic Núria Sala María José Sánchez Pérez Eva Ardanaz María-Dolores Chirlaque Richard Palmquist Thyra Löwenmark Ruth C Travis Alicia Heath Amanda J Cross Heinz Freisling Semi Zouiouich Elom Aglago Tim Waterboer David J. Hughes Association of Pre-diagnostic Antibody Responses to Escherichia coli and Bacteroides fragilis Toxin Proteins with Colorectal Cancer in a European Cohort Gut Microbes colorectal cancer escherichia coli bacteroides fragilis serology prospective |
author_facet |
Julia Butt Mazda Jenab Jill Werner Veronika Fedirko Elisabete Weiderpass Christina C. Dahm Anne Tjønneland Anja Olsen Marie-Christine Boutron-Ruault Joseph A. Rothwell Gianluca Severi Rudolf Kaaks Renée Turzanski-Fortner Krasimira Aleksandrova Matthias Schulze Domenico Palli Valeria Pala Salvatore Panico Rosario Tumino Carlotta Sacerdote Bas Bueno-de-Mesquita Carla H. Van Gils Inger Torhild Gram Marko Lukic Núria Sala María José Sánchez Pérez Eva Ardanaz María-Dolores Chirlaque Richard Palmquist Thyra Löwenmark Ruth C Travis Alicia Heath Amanda J Cross Heinz Freisling Semi Zouiouich Elom Aglago Tim Waterboer David J. Hughes |
author_sort |
Julia Butt |
title |
Association of Pre-diagnostic Antibody Responses to Escherichia coli and Bacteroides fragilis Toxin Proteins with Colorectal Cancer in a European Cohort |
title_short |
Association of Pre-diagnostic Antibody Responses to Escherichia coli and Bacteroides fragilis Toxin Proteins with Colorectal Cancer in a European Cohort |
title_full |
Association of Pre-diagnostic Antibody Responses to Escherichia coli and Bacteroides fragilis Toxin Proteins with Colorectal Cancer in a European Cohort |
title_fullStr |
Association of Pre-diagnostic Antibody Responses to Escherichia coli and Bacteroides fragilis Toxin Proteins with Colorectal Cancer in a European Cohort |
title_full_unstemmed |
Association of Pre-diagnostic Antibody Responses to Escherichia coli and Bacteroides fragilis Toxin Proteins with Colorectal Cancer in a European Cohort |
title_sort |
association of pre-diagnostic antibody responses to escherichia coli and bacteroides fragilis toxin proteins with colorectal cancer in a european cohort |
publisher |
Taylor & Francis Group |
series |
Gut Microbes |
issn |
1949-0976 1949-0984 |
publishDate |
2021-01-01 |
description |
Experimental evidence has implicated genotoxic Escherichia coli (E. coli) and enterotoxigenic Bacteroides fragilis (ETBF) in the development of colorectal cancer (CRC). However, evidence from epidemiological studies is sparse. We therefore assessed the association of serological markers of E. coli and ETBF exposure with odds of developing CRC in the European Prospective Investigation into Nutrition and Cancer (EPIC) study. Serum samples of incident CRC cases and matched controls (n = 442 pairs) were analyzed for immunoglobulin (Ig) A and G antibody responses to seven E. coli proteins and two isoforms of the ETBF toxin via multiplex serology. Multivariable-adjusted conditional logistic regression analyses were used to estimate odds ratios (ORs) and 95% confidence intervals (CIs) for the association of sero-positivity to E. coli and ETBF with CRC. The IgA-positivity of any of the tested E. coli antigens was associated with higher odds of developing CRC (OR: 1.42; 95% CI: 1.05–1.91). Dual-positivity for both IgA and IgG to E. coli and ETBF was associated with >1.7-fold higher odds of developing CRC, with a significant association only for IgG (OR: 1.75; 95% CI: 1.04, 2.94). This association was more pronounced when restricted to the proximal colon cancers (OR: 2.62; 95% CI: 1.09, 6.29) compared to those of the distal colon (OR: 1.24; 95% CI: 0.51, 3.00) (pheterogeneity = 0.095). Sero-positivity to E. coli and ETBF was associated with CRC development, suggesting that co-infection of these bacterial species may contribute to colorectal carcinogenesis. These findings warrant further exploration in larger prospective studies and within different population groups. |
topic |
colorectal cancer escherichia coli bacteroides fragilis serology prospective |
url |
http://dx.doi.org/10.1080/19490976.2021.1903825 |
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doaj-3a819d2ba7844ef68c4638dc781277a02021-07-06T12:16:09ZengTaylor & Francis GroupGut Microbes1949-09761949-09842021-01-0113110.1080/19490976.2021.19038251903825Association of Pre-diagnostic Antibody Responses to Escherichia coli and Bacteroides fragilis Toxin Proteins with Colorectal Cancer in a European CohortJulia Butt0Mazda Jenab1Jill Werner2Veronika Fedirko3Elisabete Weiderpass4Christina C. Dahm5Anne Tjønneland6Anja Olsen7Marie-Christine Boutron-Ruault8Joseph A. Rothwell9Gianluca Severi10Rudolf Kaaks11Renée Turzanski-Fortner12Krasimira Aleksandrova13Matthias Schulze14Domenico Palli15Valeria Pala16Salvatore Panico17Rosario Tumino18Carlotta Sacerdote19Bas Bueno-de-Mesquita20Carla H. Van Gils21Inger Torhild Gram22Marko Lukic23Núria Sala24María José Sánchez Pérez25Eva Ardanaz26María-Dolores Chirlaque27Richard Palmquist28Thyra Löwenmark29Ruth C Travis30Alicia Heath31Amanda J Cross32Heinz Freisling33Semi Zouiouich34Elom Aglago35Tim Waterboer36David J. Hughes37German Cancer Research Center (DKFZ)International Agency for Research on Cancer (IARC-WHO)German Cancer Research Center (DKFZ)Rollins School of Public Health, Emory UniversityAarhus UniversityRollins School of Public Health, Emory UniversityDiet, Genes and Environment, Nutrition and Biomarkers (NAB), Danish Cancer Society Research CenterDiet, Genes and Environment, Nutrition and Biomarkers (NAB), Danish Cancer Society Research CenterMédecine Université Paris-Saclay, Inserm, Gustave RoussyMédecine Université Paris-Saclay, Inserm, Gustave RoussyMédecine Université Paris-Saclay, Inserm, Gustave RoussyGerman Cancer Research Center (DKFZ)German Cancer Research Center (DKFZ)German Institute of Human Nutrition Potsdam-RehbrueckeGerman Institute of Human Nutrition, Potsdam-RehbrueckeInstitute for Cancer Research, Prevention and Clinical Network - ISPROFondazione IRCCS Istituto Nazionale Dei TumoriFederico II UniversityProvincial Health Authority (ASP 7)Città Della Salute E Della Scienza University-HospitalNational Institute for Public Health and the Environment (RIVM)University Medical Centre Utrecht, Utrecht UniversityUniversity of Tromsø, the Arctic University of NorwayThe Arctic University of NorwayCatalan Institute of Oncology (ICO), Biomedical Research Institute (IDIBELL)Escuela Andaluza De Salud Pública (EASP)Navarra Public Health InstituteRegional Health Council, IMIB-Arrixaca, Murcia UniversityUmeå UniversityUmeå UniversityUniversity of OxfordSchool of Public Health, Imperial College LondonSchool of Public Health, Imperial College LondonInternational Agency for Research on Cancer (IARC-WHO)International Agency for Research on Cancer (IARC-WHO)International Agency for Research on Cancer (IARC-WHO)German Cancer Research Center (DKFZ)University College DublinExperimental evidence has implicated genotoxic Escherichia coli (E. coli) and enterotoxigenic Bacteroides fragilis (ETBF) in the development of colorectal cancer (CRC). However, evidence from epidemiological studies is sparse. We therefore assessed the association of serological markers of E. coli and ETBF exposure with odds of developing CRC in the European Prospective Investigation into Nutrition and Cancer (EPIC) study. Serum samples of incident CRC cases and matched controls (n = 442 pairs) were analyzed for immunoglobulin (Ig) A and G antibody responses to seven E. coli proteins and two isoforms of the ETBF toxin via multiplex serology. Multivariable-adjusted conditional logistic regression analyses were used to estimate odds ratios (ORs) and 95% confidence intervals (CIs) for the association of sero-positivity to E. coli and ETBF with CRC. The IgA-positivity of any of the tested E. coli antigens was associated with higher odds of developing CRC (OR: 1.42; 95% CI: 1.05–1.91). Dual-positivity for both IgA and IgG to E. coli and ETBF was associated with >1.7-fold higher odds of developing CRC, with a significant association only for IgG (OR: 1.75; 95% CI: 1.04, 2.94). This association was more pronounced when restricted to the proximal colon cancers (OR: 2.62; 95% CI: 1.09, 6.29) compared to those of the distal colon (OR: 1.24; 95% CI: 0.51, 3.00) (pheterogeneity = 0.095). Sero-positivity to E. coli and ETBF was associated with CRC development, suggesting that co-infection of these bacterial species may contribute to colorectal carcinogenesis. These findings warrant further exploration in larger prospective studies and within different population groups.http://dx.doi.org/10.1080/19490976.2021.1903825colorectal cancerescherichia colibacteroides fragilisserologyprospective |