Efficient Plant Regeneration in Garlic through Somatic Embryogenesis from Root Tip Expiants
The present study was conducted to establish an efficient protocol of plantlet regeneration through somatic embryogenesis in garlic (Allium sativum L.). Root tips measuring 2 to 3 mm were excised and cultured on agar-solidified MS medium containing various concentrations of 2,4-dichlorophenoxyacetic...
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1998-01-01
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Online Access: | http://dx.doi.org/10.1626/pps.1.216 |
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doaj-3a8ec309b4c54462a29cf72d36ab29822020-11-25T01:42:57ZengTaylor & Francis GroupPlant Production Science1343-943X1349-10081998-01-011321622210.1626/pps.1.21611644325Efficient Plant Regeneration in Garlic through Somatic Embryogenesis from Root Tip ExpiantsMuhammad Shahidul HaqueTomikichi Wada0Kazumi Hattori1Nagoya UniversityNagoya UniversityThe present study was conducted to establish an efficient protocol of plantlet regeneration through somatic embryogenesis in garlic (Allium sativum L.). Root tips measuring 2 to 3 mm were excised and cultured on agar-solidified MS medium containing various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) for callus and embryo formation. The optimum concentration of 2,4-D was 0.5 μM. At a concentration higher than 1.0 μM, 2,4-D had an inhibitory effect on callus and embryo formation. Embryos germinated and formed rooted plantlets on MS solid medium containing 5.0 μM kinetin. The number of plantlets regenerated per root tip expiant depended on the concentration of 2,4-D in the callus initiation medium. The plantlets were established in the soil after acclimatization in a growth cabinet. Somatic embryos were morphologically characterized by scanning electron microscopy (SEM).http://dx.doi.org/10.1626/pps.1.216Allium sativum L.Callus formationGarlicRapid propagationRoot tip cultureSomatic embryogenesisTissue culture |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Muhammad Shahidul Haque Tomikichi Wada Kazumi Hattori |
spellingShingle |
Muhammad Shahidul Haque Tomikichi Wada Kazumi Hattori Efficient Plant Regeneration in Garlic through Somatic Embryogenesis from Root Tip Expiants Plant Production Science Allium sativum L. Callus formation Garlic Rapid propagation Root tip culture Somatic embryogenesis Tissue culture |
author_facet |
Muhammad Shahidul Haque Tomikichi Wada Kazumi Hattori |
author_sort |
Muhammad Shahidul Haque |
title |
Efficient Plant Regeneration in Garlic through Somatic Embryogenesis from Root Tip Expiants |
title_short |
Efficient Plant Regeneration in Garlic through Somatic Embryogenesis from Root Tip Expiants |
title_full |
Efficient Plant Regeneration in Garlic through Somatic Embryogenesis from Root Tip Expiants |
title_fullStr |
Efficient Plant Regeneration in Garlic through Somatic Embryogenesis from Root Tip Expiants |
title_full_unstemmed |
Efficient Plant Regeneration in Garlic through Somatic Embryogenesis from Root Tip Expiants |
title_sort |
efficient plant regeneration in garlic through somatic embryogenesis from root tip expiants |
publisher |
Taylor & Francis Group |
series |
Plant Production Science |
issn |
1343-943X 1349-1008 |
publishDate |
1998-01-01 |
description |
The present study was conducted to establish an efficient protocol of plantlet regeneration through somatic embryogenesis in garlic (Allium sativum L.). Root tips measuring 2 to 3 mm were excised and cultured on agar-solidified MS medium containing various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) for callus and embryo formation. The optimum concentration of 2,4-D was 0.5 μM. At a concentration higher than 1.0 μM, 2,4-D had an inhibitory effect on callus and embryo formation. Embryos germinated and formed rooted plantlets on MS solid medium containing 5.0 μM kinetin. The number of plantlets regenerated per root tip expiant depended on the concentration of 2,4-D in the callus initiation medium. The plantlets were established in the soil after acclimatization in a growth cabinet. Somatic embryos were morphologically characterized by scanning electron microscopy (SEM). |
topic |
Allium sativum L. Callus formation Garlic Rapid propagation Root tip culture Somatic embryogenesis Tissue culture |
url |
http://dx.doi.org/10.1626/pps.1.216 |
work_keys_str_mv |
AT muhammadshahidulhaque efficientplantregenerationingarlicthroughsomaticembryogenesisfromroottipexpiants AT tomikichiwada efficientplantregenerationingarlicthroughsomaticembryogenesisfromroottipexpiants AT kazumihattori efficientplantregenerationingarlicthroughsomaticembryogenesisfromroottipexpiants |
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