The regulatory role of microRNA-mRNA co-expression in hepatitis B virus-associated acute liver failure
Introduction and objectives: Acute liver failure (ALF) is a dramatic disorder requiring intensive care. MicroRNAs (miRNAs) have been identified to play important roles in ALF. This study was performed to identify miRNA-mRNA co-expression network after ALF to investigate the molecule mechanism underl...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2019-11-01
|
Series: | Annals of Hepatology |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1665268119322045 |
id |
doaj-3a97edc55995440aa4f72c9887ffb1b8 |
---|---|
record_format |
Article |
spelling |
doaj-3a97edc55995440aa4f72c9887ffb1b82021-06-09T05:56:28ZengElsevierAnnals of Hepatology1665-26812019-11-01186883892The regulatory role of microRNA-mRNA co-expression in hepatitis B virus-associated acute liver failureKanda Pan0Yunchao Wang1Ping Pan2Guanhua Xu3Lujiao Mo4Lijia Cao5Channi Wu6Xiaoyuan Shen7Department of Intensive Care Unit (ICU), The First People's Hospital of Xiaoshan District, Hangzhou, Xiaoshan District, Hangzhou, ChinaDepartment of Intensive Care Unit (ICU), The First People's Hospital of Xiaoshan District, Hangzhou, Xiaoshan District, Hangzhou, ChinaDepartment of General Medicine, The First People's Hospital of Xiaoshan District, Hangzhou, Xiaoshan District, Hangzhou, ChinaDepartment of Intensive Care Unit (ICU), The First People's Hospital of Xiaoshan District, Hangzhou, Xiaoshan District, Hangzhou, ChinaDepartment of Intensive Care Unit (ICU), The First People's Hospital of Xiaoshan District, Hangzhou, Xiaoshan District, Hangzhou, ChinaDepartment of Intensive Care Unit (ICU), The First People's Hospital of Xiaoshan District, Hangzhou, Xiaoshan District, Hangzhou, ChinaDepartment of Gastroenterology, Zhejiang Xiaoshan Hospital, Xiaoshan District, Hangzhou, China; Corresponding author.Department of Intensive Care Unit (ICU), The First People's Hospital of Xiaoshan District, Hangzhou, Xiaoshan District, Hangzhou, ChinaIntroduction and objectives: Acute liver failure (ALF) is a dramatic disorder requiring intensive care. MicroRNAs (miRNAs) have been identified to play important roles in ALF. This study was performed to identify miRNA-mRNA co-expression network after ALF to investigate the molecule mechanism underlying the pathogenesis of ALF. Materials and methods: The microarray dataset GSE62030 and GSE62029 were downloaded from Gene Expression Omnibus database. Overlapping differentially expressed miRNAs (DEmiRNAs) and genes (DEGs) were identified in liver tissues from patients with hepatitis B virus (HBV)-associated ALF in comparison with normal tissues from donors. Gene enrichment analysis was performed. Key pathways associated with the DEGs were identified. The miRNA-mRNA regulatory network was constructed. Results: Total 42 DEmiRNAs and 523 DEGs were identified in liver tissues from patients with HBV-associated ALF. Gene ontology and pathways enrichment analysis showed upregulated DEGs were related to immune responses, inflammation, and infection, and downregulated DEGs were associated with amino acids, secondary metabolites and xenobiotics metabolism. In miRNA-mRNA co-expression network, DEGs were regulated by at least one DEmiRNA and transcription factor. Further analysis showed DEmiRNAs, including has-miR-55-5p, has-miR-193b-5p, has-miR-200b-3p, and has-miR-3175 were associated with amino acid metabolism, drug metabolism and detoxication, and signaling pathways including mitogen-activated protein kinase (MAPK), phosphatidylinositol 3-kinase (PI3K)/AKT, Ras, and Rap1. Conclusions: These miRNA-mRNA pairs and changed profiles were associated with and might be responsible for the impairment of detoxification and metabolism induced by HBV-associated ALF.http://www.sciencedirect.com/science/article/pii/S1665268119322045Acute liver failureMicroRNAMicroarrayBioinformatics analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kanda Pan Yunchao Wang Ping Pan Guanhua Xu Lujiao Mo Lijia Cao Channi Wu Xiaoyuan Shen |
spellingShingle |
Kanda Pan Yunchao Wang Ping Pan Guanhua Xu Lujiao Mo Lijia Cao Channi Wu Xiaoyuan Shen The regulatory role of microRNA-mRNA co-expression in hepatitis B virus-associated acute liver failure Annals of Hepatology Acute liver failure MicroRNA Microarray Bioinformatics analysis |
author_facet |
Kanda Pan Yunchao Wang Ping Pan Guanhua Xu Lujiao Mo Lijia Cao Channi Wu Xiaoyuan Shen |
author_sort |
Kanda Pan |
title |
The regulatory role of microRNA-mRNA co-expression in hepatitis B virus-associated acute liver failure |
title_short |
The regulatory role of microRNA-mRNA co-expression in hepatitis B virus-associated acute liver failure |
title_full |
The regulatory role of microRNA-mRNA co-expression in hepatitis B virus-associated acute liver failure |
title_fullStr |
The regulatory role of microRNA-mRNA co-expression in hepatitis B virus-associated acute liver failure |
title_full_unstemmed |
The regulatory role of microRNA-mRNA co-expression in hepatitis B virus-associated acute liver failure |
title_sort |
regulatory role of microrna-mrna co-expression in hepatitis b virus-associated acute liver failure |
publisher |
Elsevier |
series |
Annals of Hepatology |
issn |
1665-2681 |
publishDate |
2019-11-01 |
description |
Introduction and objectives: Acute liver failure (ALF) is a dramatic disorder requiring intensive care. MicroRNAs (miRNAs) have been identified to play important roles in ALF. This study was performed to identify miRNA-mRNA co-expression network after ALF to investigate the molecule mechanism underlying the pathogenesis of ALF. Materials and methods: The microarray dataset GSE62030 and GSE62029 were downloaded from Gene Expression Omnibus database. Overlapping differentially expressed miRNAs (DEmiRNAs) and genes (DEGs) were identified in liver tissues from patients with hepatitis B virus (HBV)-associated ALF in comparison with normal tissues from donors. Gene enrichment analysis was performed. Key pathways associated with the DEGs were identified. The miRNA-mRNA regulatory network was constructed. Results: Total 42 DEmiRNAs and 523 DEGs were identified in liver tissues from patients with HBV-associated ALF. Gene ontology and pathways enrichment analysis showed upregulated DEGs were related to immune responses, inflammation, and infection, and downregulated DEGs were associated with amino acids, secondary metabolites and xenobiotics metabolism. In miRNA-mRNA co-expression network, DEGs were regulated by at least one DEmiRNA and transcription factor. Further analysis showed DEmiRNAs, including has-miR-55-5p, has-miR-193b-5p, has-miR-200b-3p, and has-miR-3175 were associated with amino acid metabolism, drug metabolism and detoxication, and signaling pathways including mitogen-activated protein kinase (MAPK), phosphatidylinositol 3-kinase (PI3K)/AKT, Ras, and Rap1. Conclusions: These miRNA-mRNA pairs and changed profiles were associated with and might be responsible for the impairment of detoxification and metabolism induced by HBV-associated ALF. |
topic |
Acute liver failure MicroRNA Microarray Bioinformatics analysis |
url |
http://www.sciencedirect.com/science/article/pii/S1665268119322045 |
work_keys_str_mv |
AT kandapan theregulatoryroleofmicrornamrnacoexpressioninhepatitisbvirusassociatedacuteliverfailure AT yunchaowang theregulatoryroleofmicrornamrnacoexpressioninhepatitisbvirusassociatedacuteliverfailure AT pingpan theregulatoryroleofmicrornamrnacoexpressioninhepatitisbvirusassociatedacuteliverfailure AT guanhuaxu theregulatoryroleofmicrornamrnacoexpressioninhepatitisbvirusassociatedacuteliverfailure AT lujiaomo theregulatoryroleofmicrornamrnacoexpressioninhepatitisbvirusassociatedacuteliverfailure AT lijiacao theregulatoryroleofmicrornamrnacoexpressioninhepatitisbvirusassociatedacuteliverfailure AT channiwu theregulatoryroleofmicrornamrnacoexpressioninhepatitisbvirusassociatedacuteliverfailure AT xiaoyuanshen theregulatoryroleofmicrornamrnacoexpressioninhepatitisbvirusassociatedacuteliverfailure AT kandapan regulatoryroleofmicrornamrnacoexpressioninhepatitisbvirusassociatedacuteliverfailure AT yunchaowang regulatoryroleofmicrornamrnacoexpressioninhepatitisbvirusassociatedacuteliverfailure AT pingpan regulatoryroleofmicrornamrnacoexpressioninhepatitisbvirusassociatedacuteliverfailure AT guanhuaxu regulatoryroleofmicrornamrnacoexpressioninhepatitisbvirusassociatedacuteliverfailure AT lujiaomo regulatoryroleofmicrornamrnacoexpressioninhepatitisbvirusassociatedacuteliverfailure AT lijiacao regulatoryroleofmicrornamrnacoexpressioninhepatitisbvirusassociatedacuteliverfailure AT channiwu regulatoryroleofmicrornamrnacoexpressioninhepatitisbvirusassociatedacuteliverfailure AT xiaoyuanshen regulatoryroleofmicrornamrnacoexpressioninhepatitisbvirusassociatedacuteliverfailure |
_version_ |
1721388560307716096 |