The Essential Role of anxA2 in Langerhans Cell Birbeck Granules Formation
Langerhans cells (LC) are the resident antigen presenting cells of the mucosal epithelium and play an essential role in initiating immune responses. LC are the only cells in the body to contain Birbeck granules (BG), which are unique cytoplasmic organelles comprised of c-type lectin langerin. Studie...
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doaj-3a9a5dd185884380a66438db682bb79e2020-11-25T02:30:44ZengMDPI AGCells2073-44092020-04-01997497410.3390/cells9040974The Essential Role of anxA2 in Langerhans Cell Birbeck Granules FormationShantae M. Thornton0Varsha D. Samararatne1Joseph G. Skeate2Christopher Buser3Kim P. Lühen4Julia R. Taylor5Diane M. Da Silva6W. Martin Kast7Department of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA 90033, USADepartment of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA 90033, USADepartment of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA 90033, USAOak Crest Institute of Science, Monrovia, CA 91016, USANorris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90033, USADepartment of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA 90033, USANorris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90033, USADepartment of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA 90033, USALangerhans cells (LC) are the resident antigen presenting cells of the mucosal epithelium and play an essential role in initiating immune responses. LC are the only cells in the body to contain Birbeck granules (BG), which are unique cytoplasmic organelles comprised of c-type lectin langerin. Studies of BG have historically focused on morphological characterizations, but BG have also been implicated in viral antigen processing which suggests that they can serve a function in antiviral immunity. This study focused on investigating proteins that could be involved in BG formation to further characterize their structure using transmission electron microscopy (TEM). Here, we report a critical role for the protein annexin A2 (anxA2) in the proper formation of BG structures. When anxA2 expression is downregulated, langerin expression decreases, cytoplasmic BG are nearly ablated, and the presence of malformed BG-like structures increases. Furthermore, in the absence of anxA2, we found langerin was no longer localized to BG or BG-like structures. Taken together, these results indicate an essential role for anxA2 in facilitating the proper formation of BG.https://www.mdpi.com/2073-4409/9/4/974anxA2Birbeck granulesLangerhans cellA2t |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shantae M. Thornton Varsha D. Samararatne Joseph G. Skeate Christopher Buser Kim P. Lühen Julia R. Taylor Diane M. Da Silva W. Martin Kast |
spellingShingle |
Shantae M. Thornton Varsha D. Samararatne Joseph G. Skeate Christopher Buser Kim P. Lühen Julia R. Taylor Diane M. Da Silva W. Martin Kast The Essential Role of anxA2 in Langerhans Cell Birbeck Granules Formation Cells anxA2 Birbeck granules Langerhans cell A2t |
author_facet |
Shantae M. Thornton Varsha D. Samararatne Joseph G. Skeate Christopher Buser Kim P. Lühen Julia R. Taylor Diane M. Da Silva W. Martin Kast |
author_sort |
Shantae M. Thornton |
title |
The Essential Role of anxA2 in Langerhans Cell Birbeck Granules Formation |
title_short |
The Essential Role of anxA2 in Langerhans Cell Birbeck Granules Formation |
title_full |
The Essential Role of anxA2 in Langerhans Cell Birbeck Granules Formation |
title_fullStr |
The Essential Role of anxA2 in Langerhans Cell Birbeck Granules Formation |
title_full_unstemmed |
The Essential Role of anxA2 in Langerhans Cell Birbeck Granules Formation |
title_sort |
essential role of anxa2 in langerhans cell birbeck granules formation |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2020-04-01 |
description |
Langerhans cells (LC) are the resident antigen presenting cells of the mucosal epithelium and play an essential role in initiating immune responses. LC are the only cells in the body to contain Birbeck granules (BG), which are unique cytoplasmic organelles comprised of c-type lectin langerin. Studies of BG have historically focused on morphological characterizations, but BG have also been implicated in viral antigen processing which suggests that they can serve a function in antiviral immunity. This study focused on investigating proteins that could be involved in BG formation to further characterize their structure using transmission electron microscopy (TEM). Here, we report a critical role for the protein annexin A2 (anxA2) in the proper formation of BG structures. When anxA2 expression is downregulated, langerin expression decreases, cytoplasmic BG are nearly ablated, and the presence of malformed BG-like structures increases. Furthermore, in the absence of anxA2, we found langerin was no longer localized to BG or BG-like structures. Taken together, these results indicate an essential role for anxA2 in facilitating the proper formation of BG. |
topic |
anxA2 Birbeck granules Langerhans cell A2t |
url |
https://www.mdpi.com/2073-4409/9/4/974 |
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