Activation of Pro-apoptotic Caspases in Non-apoptotic Cells During Odontogenesis and Related Osteogenesis
Caspases are well known proteases in the context of inflammation and apoptosis. Recently, novel roles of pro-apoptotic caspases have been reported, including findings related to the development of hard tissues. To further investigate these emerging functions of pro-apoptotic caspases, the in vivo lo...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2018-03-01
|
Series: | Frontiers in Physiology |
Subjects: | |
Online Access: | http://journal.frontiersin.org/article/10.3389/fphys.2018.00174/full |
id |
doaj-3aab8a7c311c4af490bce96c95af367f |
---|---|
record_format |
Article |
spelling |
doaj-3aab8a7c311c4af490bce96c95af367f2020-11-24T23:49:23ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-03-01910.3389/fphys.2018.00174312644Activation of Pro-apoptotic Caspases in Non-apoptotic Cells During Odontogenesis and Related OsteogenesisEva Svandova0Eva Svandova1Barbora Vesela2Barbora Vesela3Abigail S. Tucker4Eva Matalova5Eva Matalova6Department of Physiology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, CzechiaLaboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Brno, CzechiaDepartment of Physiology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, CzechiaLaboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Brno, CzechiaDepartment of Craniofacial Development and Stem Cell Research, King's College London, London, United KingdomDepartment of Physiology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, CzechiaLaboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Brno, CzechiaCaspases are well known proteases in the context of inflammation and apoptosis. Recently, novel roles of pro-apoptotic caspases have been reported, including findings related to the development of hard tissues. To further investigate these emerging functions of pro-apoptotic caspases, the in vivo localisation of key pro-apoptotic caspases (-3,-6,-7,-8, and -9) was assessed, concentrating on the development of two neighbouring hard tissues, cells participating in odontogenesis (represented by the first mouse molar) and intramembranous osteogenesis (mandibular/alveolar bone). The expression of the different caspases within the developing tissues was correlated with the apoptotic status of the cells, to produce a picture of whether different caspases have potentially distinct, or overlapping non-apoptotic functions. The in vivo investigation was additionally supported by examination of caspases in an osteoblast-like cell line in vitro. Caspases-3,-7, and -9 were activated in apoptotic cells of the primary enamel knot of the first molar; however, caspase-7 and -8 activation was also associated with the non-apoptotic enamel epithelium at the same stage and later with differentiating/differentiated odontoblasts and ameloblasts. In the adjacent bone, active caspases-7 and -8 were present abundantly in the prenatal period, while the appearance of caspases-3,-6, and -9 was marginal. Perinatally, caspases-3 and -7 were evident in some osteoclasts and osteoblastic cells, and caspase-8 was abundant mostly in osteoclasts. In addition, postnatal activation of caspases-7 and -8 was retained in osteocytes. The results provide a comprehensive temporo-spatial pattern of pro-apoptotic caspase activation, and demonstrate both unique and overlapping activation in non-apoptotic cells during development of the molar tooth and mandibular/alveolar bone. The importance of caspases in osteogenic pathways is highlighted by caspase inhibition in osteoblast-like cells, which led to a significant decrease in osteocalcin expression, supporting a role in hard tissue cell differentiation.http://journal.frontiersin.org/article/10.3389/fphys.2018.00174/fullcaspasedifferentiationapoptosistoothintramembranousbone |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eva Svandova Eva Svandova Barbora Vesela Barbora Vesela Abigail S. Tucker Eva Matalova Eva Matalova |
spellingShingle |
Eva Svandova Eva Svandova Barbora Vesela Barbora Vesela Abigail S. Tucker Eva Matalova Eva Matalova Activation of Pro-apoptotic Caspases in Non-apoptotic Cells During Odontogenesis and Related Osteogenesis Frontiers in Physiology caspase differentiation apoptosis tooth intramembranous bone |
author_facet |
Eva Svandova Eva Svandova Barbora Vesela Barbora Vesela Abigail S. Tucker Eva Matalova Eva Matalova |
author_sort |
Eva Svandova |
title |
Activation of Pro-apoptotic Caspases in Non-apoptotic Cells During Odontogenesis and Related Osteogenesis |
title_short |
Activation of Pro-apoptotic Caspases in Non-apoptotic Cells During Odontogenesis and Related Osteogenesis |
title_full |
Activation of Pro-apoptotic Caspases in Non-apoptotic Cells During Odontogenesis and Related Osteogenesis |
title_fullStr |
Activation of Pro-apoptotic Caspases in Non-apoptotic Cells During Odontogenesis and Related Osteogenesis |
title_full_unstemmed |
Activation of Pro-apoptotic Caspases in Non-apoptotic Cells During Odontogenesis and Related Osteogenesis |
title_sort |
activation of pro-apoptotic caspases in non-apoptotic cells during odontogenesis and related osteogenesis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Physiology |
issn |
1664-042X |
publishDate |
2018-03-01 |
description |
Caspases are well known proteases in the context of inflammation and apoptosis. Recently, novel roles of pro-apoptotic caspases have been reported, including findings related to the development of hard tissues. To further investigate these emerging functions of pro-apoptotic caspases, the in vivo localisation of key pro-apoptotic caspases (-3,-6,-7,-8, and -9) was assessed, concentrating on the development of two neighbouring hard tissues, cells participating in odontogenesis (represented by the first mouse molar) and intramembranous osteogenesis (mandibular/alveolar bone). The expression of the different caspases within the developing tissues was correlated with the apoptotic status of the cells, to produce a picture of whether different caspases have potentially distinct, or overlapping non-apoptotic functions. The in vivo investigation was additionally supported by examination of caspases in an osteoblast-like cell line in vitro. Caspases-3,-7, and -9 were activated in apoptotic cells of the primary enamel knot of the first molar; however, caspase-7 and -8 activation was also associated with the non-apoptotic enamel epithelium at the same stage and later with differentiating/differentiated odontoblasts and ameloblasts. In the adjacent bone, active caspases-7 and -8 were present abundantly in the prenatal period, while the appearance of caspases-3,-6, and -9 was marginal. Perinatally, caspases-3 and -7 were evident in some osteoclasts and osteoblastic cells, and caspase-8 was abundant mostly in osteoclasts. In addition, postnatal activation of caspases-7 and -8 was retained in osteocytes. The results provide a comprehensive temporo-spatial pattern of pro-apoptotic caspase activation, and demonstrate both unique and overlapping activation in non-apoptotic cells during development of the molar tooth and mandibular/alveolar bone. The importance of caspases in osteogenic pathways is highlighted by caspase inhibition in osteoblast-like cells, which led to a significant decrease in osteocalcin expression, supporting a role in hard tissue cell differentiation. |
topic |
caspase differentiation apoptosis tooth intramembranous bone |
url |
http://journal.frontiersin.org/article/10.3389/fphys.2018.00174/full |
work_keys_str_mv |
AT evasvandova activationofproapoptoticcaspasesinnonapoptoticcellsduringodontogenesisandrelatedosteogenesis AT evasvandova activationofproapoptoticcaspasesinnonapoptoticcellsduringodontogenesisandrelatedosteogenesis AT barboravesela activationofproapoptoticcaspasesinnonapoptoticcellsduringodontogenesisandrelatedosteogenesis AT barboravesela activationofproapoptoticcaspasesinnonapoptoticcellsduringodontogenesisandrelatedosteogenesis AT abigailstucker activationofproapoptoticcaspasesinnonapoptoticcellsduringodontogenesisandrelatedosteogenesis AT evamatalova activationofproapoptoticcaspasesinnonapoptoticcellsduringodontogenesisandrelatedosteogenesis AT evamatalova activationofproapoptoticcaspasesinnonapoptoticcellsduringodontogenesisandrelatedosteogenesis |
_version_ |
1725482510249361408 |