Comparative Evaluation of Infected and Noninfected Amblyomma triste Ticks with Rickettsia parkeri, the Agent of an Emerging Rickettsiosis in the New World
The distribution of Rickettsia parkeri in South America has been associated with Amblyomma triste ticks. The present study evaluated under laboratory conditions two colonies of A. triste: one started from engorged females that were naturally infected by R. parkeri (designated as infected group); the...
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doaj-3ab19ba2435244beb97188a74455a8322020-11-24T21:13:25ZengHindawi LimitedBioMed Research International2314-61332314-61412013-01-01201310.1155/2013/402737402737Comparative Evaluation of Infected and Noninfected Amblyomma triste Ticks with Rickettsia parkeri, the Agent of an Emerging Rickettsiosis in the New WorldF. A. Nieri-Bastos0M. P. J. Szabó1R. C. Pacheco2J. F. Soares3H. S. Soares4J. Moraes-Filho5R. A. Dias6M. B. Labruna7Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Avenida Prof. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, BrazilFaculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Uberlândia, Avenida Pará 1720, Campus Umuarama-Bloco 2T, 38400-902 Uberlândia, MG, BrazilFaculdade de Agronomia e Medicina Veterinária, Universidade Federal do Mato Grosso, Avenida Fernando Corrêa da Costa 2367, Boa Esperança, 78060-900 Cuiabá, MT, BrazilFaculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Avenida Prof. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, BrazilFaculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Avenida Prof. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, BrazilFaculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Avenida Prof. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, BrazilFaculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Avenida Prof. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, BrazilFaculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Avenida Prof. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, BrazilThe distribution of Rickettsia parkeri in South America has been associated with Amblyomma triste ticks. The present study evaluated under laboratory conditions two colonies of A. triste: one started from engorged females that were naturally infected by R. parkeri (designated as infected group); the other started from noninfected females (designated as control group). Both colonies were reared in parallel for five consecutive generations. Tick-naïve domestic rabbits were used for feeding of each tick stage and generation. R. parkeri was preserved by transstadial maintenance and transovarial transmission in A. triste ticks for five consecutive generations, because all tested larvae, nymphs, and adults from the infected group were shown by PCR to contain rickettsial DNA. All rabbits infested by larvae, nymphs, and adults from the infected group seroconverted, indicating that these tick stages were all vector competent for R. parkeri. Expressive differences in mortality rates were observed between engorged nymphs from the infected and control groups, as indicated by 65.9% and 92.4% molting success, respectively. Our results indicate that A. triste can act as a natural reservoir for R. parkeri. However, due to deleterious effect caused by R. parkeri on engorged nymphs, amplifier vertebrate hosts might be necessary for natural long-term maintenance of R. parkeri in A. triste.http://dx.doi.org/10.1155/2013/402737 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
F. A. Nieri-Bastos M. P. J. Szabó R. C. Pacheco J. F. Soares H. S. Soares J. Moraes-Filho R. A. Dias M. B. Labruna |
spellingShingle |
F. A. Nieri-Bastos M. P. J. Szabó R. C. Pacheco J. F. Soares H. S. Soares J. Moraes-Filho R. A. Dias M. B. Labruna Comparative Evaluation of Infected and Noninfected Amblyomma triste Ticks with Rickettsia parkeri, the Agent of an Emerging Rickettsiosis in the New World BioMed Research International |
author_facet |
F. A. Nieri-Bastos M. P. J. Szabó R. C. Pacheco J. F. Soares H. S. Soares J. Moraes-Filho R. A. Dias M. B. Labruna |
author_sort |
F. A. Nieri-Bastos |
title |
Comparative Evaluation of Infected and Noninfected Amblyomma triste Ticks with Rickettsia parkeri, the Agent of an Emerging Rickettsiosis in the New World |
title_short |
Comparative Evaluation of Infected and Noninfected Amblyomma triste Ticks with Rickettsia parkeri, the Agent of an Emerging Rickettsiosis in the New World |
title_full |
Comparative Evaluation of Infected and Noninfected Amblyomma triste Ticks with Rickettsia parkeri, the Agent of an Emerging Rickettsiosis in the New World |
title_fullStr |
Comparative Evaluation of Infected and Noninfected Amblyomma triste Ticks with Rickettsia parkeri, the Agent of an Emerging Rickettsiosis in the New World |
title_full_unstemmed |
Comparative Evaluation of Infected and Noninfected Amblyomma triste Ticks with Rickettsia parkeri, the Agent of an Emerging Rickettsiosis in the New World |
title_sort |
comparative evaluation of infected and noninfected amblyomma triste ticks with rickettsia parkeri, the agent of an emerging rickettsiosis in the new world |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2013-01-01 |
description |
The distribution of Rickettsia parkeri in South America has been associated with Amblyomma triste ticks. The present study evaluated under laboratory conditions two colonies of A. triste: one started from engorged females that were naturally infected by R. parkeri (designated as infected group); the other started from noninfected females (designated as control group). Both colonies were reared in parallel for five consecutive generations. Tick-naïve domestic rabbits were used for feeding of each tick stage and generation. R. parkeri was preserved by transstadial maintenance and transovarial transmission in A. triste ticks for five consecutive generations, because all tested larvae, nymphs, and adults from the infected group were shown by PCR to contain rickettsial DNA. All rabbits infested by larvae, nymphs, and adults from the infected group seroconverted, indicating that these tick stages were all vector competent for R. parkeri. Expressive differences in mortality rates were observed between engorged nymphs from the infected and control groups, as indicated by 65.9% and 92.4% molting success, respectively. Our results indicate that A. triste can act as a natural reservoir for R. parkeri. However, due to deleterious effect caused by R. parkeri on engorged nymphs, amplifier vertebrate hosts might be necessary for natural long-term maintenance of R. parkeri in A. triste. |
url |
http://dx.doi.org/10.1155/2013/402737 |
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