Survey and Diversity of <i>Grapevine Pinot gris virus</i> in Algeria and Comprehensive High-Throughput Small RNA Sequencing Analysis of Two Isolates from <i>Vitis vinifera</i> cv. Sabel Revealing High Viral Diversity

• Main Authors: Aleš Eichmeier, Eliška Peňázová, Jana Čechová, Akila Berraf-Tebbal
• Format: Article
• Language: English
• Published: MDPI AG 2020-09-01
• Series: Genes
• Subjects: high-throughput small RNA sequencing; grapevine; RT-PCR; <i>Grapevine Pinot gris virus</i>; RdRp
• Online Access: https://www.mdpi.com/2073-4425/11/9/1110

<i>Grapevine Pinot gris virus</i> (GPGV) is a putative causal agent of grapevine leaf mottling and deformation disease that has been reported worldwide throughout the grapevine-growing regions. Fifty-four grapevines collected from five Algerian grapevine-growing regions were tested for the presence of GPGV in phloem tissues. Eight of the tested grapevines were infected by GPGV. Viromes of two selected <i>Vitis vinifera</i> cv. Sabel grapevines infected by GPGV and showing virus-like symptoms were analyzed by small RNA sequencing. Phylogenetic analyses of the partial coding sequence (cds) of the RNA-dependent RNA polymerase (RdRp) domain showed that all Algerian GPGV isolates were grouped with some already-described asymptomatic isolates. This study provides the first survey of the occurrence of GPGV in Algeria. Moreover, <i>Grapevine fleck virus</i>, <i>Grapevine rupestris stem pitting-associated virus</i>, <i>Grapevine virus B</i>, <i>Grapevine rupestris vein feathering virus</i>, <i>Hop stunt viroid</i> and <i>Grapevine yellow speckle viroid 1</i> were detected in Algeria for the first time.