A comparative study of protocols for mouse embryonic stem cell culturing.

Most stem cell laboratories still rely on old culture methods to support the expansion and maintenance of mouse embryonic stem (ES) cells. These involve growing cells on mouse embryonic fibroblast feeder cells or on gelatin in media supplemented with fetal bovine serum and leukemia inhibitory factor...

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Bibliographic Details
Main Authors: Christoffer Tamm, Sara Pijuan Galitó, Cecilia Annerén
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3858223?pdf=render