Characterization Of blaNDM-5-Positive Escherichia coli Prevalent In A University Hospital In Eastern China

Pengfei Sun,1,2,* Wenying Xia,1,2,* Genyan Liu,1,2 Xu Huang,1,2 Chenjie Tang,1,2 Chengcheng Liu,1,2 Yuqiao Xu,1,2 Fang Ni,1,2 Yaning Mei,1,2 Shiyang Pan1,2 1Department of Laboratory Medicine, Jiangsu Province Hospital and Nanjing Medical University First Affiliated Hospital, Nanjing, People&rsqu...

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Main Authors: Sun P, Xia W, Liu G, Huang X, Tang C, Liu C, Xu Y, Ni F, Mei Y, Pan S
Format: Article
Language:English
Published: Dove Medical Press 2019-09-01
Series:Infection and Drug Resistance
Subjects:
Online Access:https://www.dovepress.com/characterization-of-blandm-5-positive-escherichia-coli-prevalent-in-a--peer-reviewed-article-IDR
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spelling doaj-3ad2fb6558d3415082346f3978f67ce22020-11-25T01:39:51ZengDove Medical PressInfection and Drug Resistance1178-69732019-09-01Volume 123029303848780Characterization Of blaNDM-5-Positive Escherichia coli Prevalent In A University Hospital In Eastern ChinaSun PXia WLiu GHuang XTang CLiu CXu YNi FMei YPan SPengfei Sun,1,2,* Wenying Xia,1,2,* Genyan Liu,1,2 Xu Huang,1,2 Chenjie Tang,1,2 Chengcheng Liu,1,2 Yuqiao Xu,1,2 Fang Ni,1,2 Yaning Mei,1,2 Shiyang Pan1,2 1Department of Laboratory Medicine, Jiangsu Province Hospital and Nanjing Medical University First Affiliated Hospital, Nanjing, People’s Republic of China; 2National Key Clinical Department of Laboratory Medicine, Nanjing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Genyan LiuDepartment of Laboratory Medicine, Jiangsu Province Hospital, Guangzhou Street No. 300, Nanjing 210029, People’s Republic of ChinaTel +8625 6830 7332Fax +8625 8372 4440Email liugenyan@njmu.edu.cnPurpose: The emergence and spread of carbapenem-resistant Enterobacteriaceae deserves special concern worldwide. Unlike the epidemiological characteristics reported in other studies, we found that the production of New Delhi metallo-β-lactamase 5 was the main mechanism for the resistance of Escherichia coli to carbapenems.Methods: All carbapenem-resistant strains were collected from July 2017 to July 2018 of the First Affiliated Hospital of Nanjing Medical University. The presence of carbapenemase-encoding genes was detected using PCR and gene sequencing. Genetic relatedness of the blaNDM-5-positive E. coli strains was determined with PFGE and MLST. Susceptibility profiles were measured with broth microdilution method and E-test strips. Transferability features of blaNDM-5 gene were assessed by conjugation experiments, S1-PFGE, southern blotting and PCR-based replicon typing methods. The genetic structures surrounding blaNDM-5 were acquired by whole genome sequencing and PCR mapping.Results: Among the 28 carbapenem-resistant E. coli strains, 18 (64%) were verified as NDM-5 producers. The 18 blaNDM-5-positive E. coli strains showed high resistance to most antibiotics, but 100% were sensitive to colistin and tigecycline. In addition, the 18 blaNDM-5-positive E. coli strains belonged to eight STs, among which ST167, ST410 and ST101 were found to cause clonal spread in the hospital. Further studies found that the blaNDM-5 gene was located on an IncX3-type plasmid, and all plasmids harbored an IS3000-ΔISAba125-IS5-blaNDM-5-bleMBL-trpF-dsbC-IS26 structure.Conclusion: The clonal spread of blaNDM-5-positive E. coli strains and horizontal dissemination via the pNDM-MGR 194-like plasmids should draw more attention. Appropriate infection control operations should be performed to prevent the further spread of blaNDM-5.Keywords: Escherichia coli, carbapenem-resistant Enterobacteriaceae, New Delhi metallo-β-lactamase, blaNDM-5, IncX3 type plasmidhttps://www.dovepress.com/characterization-of-blandm-5-positive-escherichia-coli-prevalent-in-a--peer-reviewed-article-IDREscherichia colicarbapenem-resistant EnterobacteriaceaeNew Delhi metallo-β-lactamaseblaNDM-5IncX3 type plasmid
collection DOAJ
language English
format Article
sources DOAJ
author Sun P
Xia W
Liu G
Huang X
Tang C
Liu C
Xu Y
Ni F
Mei Y
Pan S
spellingShingle Sun P
Xia W
Liu G
Huang X
Tang C
Liu C
Xu Y
Ni F
Mei Y
Pan S
Characterization Of blaNDM-5-Positive Escherichia coli Prevalent In A University Hospital In Eastern China
Infection and Drug Resistance
Escherichia coli
carbapenem-resistant Enterobacteriaceae
New Delhi metallo-β-lactamase
blaNDM-5
IncX3 type plasmid
author_facet Sun P
Xia W
Liu G
Huang X
Tang C
Liu C
Xu Y
Ni F
Mei Y
Pan S
author_sort Sun P
title Characterization Of blaNDM-5-Positive Escherichia coli Prevalent In A University Hospital In Eastern China
title_short Characterization Of blaNDM-5-Positive Escherichia coli Prevalent In A University Hospital In Eastern China
title_full Characterization Of blaNDM-5-Positive Escherichia coli Prevalent In A University Hospital In Eastern China
title_fullStr Characterization Of blaNDM-5-Positive Escherichia coli Prevalent In A University Hospital In Eastern China
title_full_unstemmed Characterization Of blaNDM-5-Positive Escherichia coli Prevalent In A University Hospital In Eastern China
title_sort characterization of blandm-5-positive escherichia coli prevalent in a university hospital in eastern china
publisher Dove Medical Press
series Infection and Drug Resistance
issn 1178-6973
publishDate 2019-09-01
description Pengfei Sun,1,2,* Wenying Xia,1,2,* Genyan Liu,1,2 Xu Huang,1,2 Chenjie Tang,1,2 Chengcheng Liu,1,2 Yuqiao Xu,1,2 Fang Ni,1,2 Yaning Mei,1,2 Shiyang Pan1,2 1Department of Laboratory Medicine, Jiangsu Province Hospital and Nanjing Medical University First Affiliated Hospital, Nanjing, People’s Republic of China; 2National Key Clinical Department of Laboratory Medicine, Nanjing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Genyan LiuDepartment of Laboratory Medicine, Jiangsu Province Hospital, Guangzhou Street No. 300, Nanjing 210029, People’s Republic of ChinaTel +8625 6830 7332Fax +8625 8372 4440Email liugenyan@njmu.edu.cnPurpose: The emergence and spread of carbapenem-resistant Enterobacteriaceae deserves special concern worldwide. Unlike the epidemiological characteristics reported in other studies, we found that the production of New Delhi metallo-β-lactamase 5 was the main mechanism for the resistance of Escherichia coli to carbapenems.Methods: All carbapenem-resistant strains were collected from July 2017 to July 2018 of the First Affiliated Hospital of Nanjing Medical University. The presence of carbapenemase-encoding genes was detected using PCR and gene sequencing. Genetic relatedness of the blaNDM-5-positive E. coli strains was determined with PFGE and MLST. Susceptibility profiles were measured with broth microdilution method and E-test strips. Transferability features of blaNDM-5 gene were assessed by conjugation experiments, S1-PFGE, southern blotting and PCR-based replicon typing methods. The genetic structures surrounding blaNDM-5 were acquired by whole genome sequencing and PCR mapping.Results: Among the 28 carbapenem-resistant E. coli strains, 18 (64%) were verified as NDM-5 producers. The 18 blaNDM-5-positive E. coli strains showed high resistance to most antibiotics, but 100% were sensitive to colistin and tigecycline. In addition, the 18 blaNDM-5-positive E. coli strains belonged to eight STs, among which ST167, ST410 and ST101 were found to cause clonal spread in the hospital. Further studies found that the blaNDM-5 gene was located on an IncX3-type plasmid, and all plasmids harbored an IS3000-ΔISAba125-IS5-blaNDM-5-bleMBL-trpF-dsbC-IS26 structure.Conclusion: The clonal spread of blaNDM-5-positive E. coli strains and horizontal dissemination via the pNDM-MGR 194-like plasmids should draw more attention. Appropriate infection control operations should be performed to prevent the further spread of blaNDM-5.Keywords: Escherichia coli, carbapenem-resistant Enterobacteriaceae, New Delhi metallo-β-lactamase, blaNDM-5, IncX3 type plasmid
topic Escherichia coli
carbapenem-resistant Enterobacteriaceae
New Delhi metallo-β-lactamase
blaNDM-5
IncX3 type plasmid
url https://www.dovepress.com/characterization-of-blandm-5-positive-escherichia-coli-prevalent-in-a--peer-reviewed-article-IDR
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