Adipose Tissue-Derived Stem Cell Yield Depends on Isolation Protocol and Cell Counting Method
In plastic surgery, lipofilling is a frequent procedure. Unsatisfactory vascularization and impaired cell vitality can lead to unpredictable take rates in the fat graft. The proliferation and neovascularization inducing properties of adipose tissue-derived stem cells may contribute to solve this pro...
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doaj-3ad71fda58c44cc3927ba93a69a170bf2021-05-31T23:15:44ZengMDPI AGCells2073-44092021-05-01101113111310.3390/cells10051113Adipose Tissue-Derived Stem Cell Yield Depends on Isolation Protocol and Cell Counting MethodLukas Prantl0Andreas Eigenberger1Eva Brix2Sally Kempa3Magnus Baringer4Oliver Felthaus5Department of Plastic, Hand and Reconstructive Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, GermanyDepartment of Plastic, Hand and Reconstructive Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, GermanyDepartment of Plastic, Hand and Reconstructive Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, GermanyDepartment of Plastic, Hand and Reconstructive Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, GermanyDepartment of Plastic, Hand and Reconstructive Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, GermanyDepartment of Plastic, Hand and Reconstructive Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, GermanyIn plastic surgery, lipofilling is a frequent procedure. Unsatisfactory vascularization and impaired cell vitality can lead to unpredictable take rates in the fat graft. The proliferation and neovascularization inducing properties of adipose tissue-derived stem cells may contribute to solve this problem. Therefore, the enrichment of fat grafts with stem cells is studied intensively. However, it is difficult to compare these studies because many factors—often not precisely described—are influencing the results. Our study summarizes some factors which influence the cell yield like harvesting, isolation procedure and quantification. Stem cells were isolated after liposuction. Quantification was done using a cell chamber, colony counting, or flow cytometry with changes to one parameter, only, for each comparison. Quantification of cells isolated after liposuction at the same harvesting site from the same patient can vary greatly depending on the details of the isolation protocol and the method of quantification. Cell yield can be influenced strongly by many factors. Therefore, a comparison of different studies should be handled with care.https://www.mdpi.com/2073-4409/10/5/1113plastic surgeryadipose tissuelipoaspiratestem cellsfat graftingcell yield |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lukas Prantl Andreas Eigenberger Eva Brix Sally Kempa Magnus Baringer Oliver Felthaus |
spellingShingle |
Lukas Prantl Andreas Eigenberger Eva Brix Sally Kempa Magnus Baringer Oliver Felthaus Adipose Tissue-Derived Stem Cell Yield Depends on Isolation Protocol and Cell Counting Method Cells plastic surgery adipose tissue lipoaspirate stem cells fat grafting cell yield |
author_facet |
Lukas Prantl Andreas Eigenberger Eva Brix Sally Kempa Magnus Baringer Oliver Felthaus |
author_sort |
Lukas Prantl |
title |
Adipose Tissue-Derived Stem Cell Yield Depends on Isolation Protocol and Cell Counting Method |
title_short |
Adipose Tissue-Derived Stem Cell Yield Depends on Isolation Protocol and Cell Counting Method |
title_full |
Adipose Tissue-Derived Stem Cell Yield Depends on Isolation Protocol and Cell Counting Method |
title_fullStr |
Adipose Tissue-Derived Stem Cell Yield Depends on Isolation Protocol and Cell Counting Method |
title_full_unstemmed |
Adipose Tissue-Derived Stem Cell Yield Depends on Isolation Protocol and Cell Counting Method |
title_sort |
adipose tissue-derived stem cell yield depends on isolation protocol and cell counting method |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2021-05-01 |
description |
In plastic surgery, lipofilling is a frequent procedure. Unsatisfactory vascularization and impaired cell vitality can lead to unpredictable take rates in the fat graft. The proliferation and neovascularization inducing properties of adipose tissue-derived stem cells may contribute to solve this problem. Therefore, the enrichment of fat grafts with stem cells is studied intensively. However, it is difficult to compare these studies because many factors—often not precisely described—are influencing the results. Our study summarizes some factors which influence the cell yield like harvesting, isolation procedure and quantification. Stem cells were isolated after liposuction. Quantification was done using a cell chamber, colony counting, or flow cytometry with changes to one parameter, only, for each comparison. Quantification of cells isolated after liposuction at the same harvesting site from the same patient can vary greatly depending on the details of the isolation protocol and the method of quantification. Cell yield can be influenced strongly by many factors. Therefore, a comparison of different studies should be handled with care. |
topic |
plastic surgery adipose tissue lipoaspirate stem cells fat grafting cell yield |
url |
https://www.mdpi.com/2073-4409/10/5/1113 |
work_keys_str_mv |
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