Effects of Isorhamnetin in Human Amniotic Epithelial Stem Cells in vitro and Its Cardioprotective Effects in vivo
Cardiac hypertrophy and fibrosis are major pathophysiologic disorders that lead to serious cardiovascular diseases (CVDs), such as heart failure and arrhythmia. It is well known that transforming growth factor β (TGFβ) signaling pathways play a major role in the proliferation of cardiac hypertrophy...
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doaj-3af4bb5c73b2449a92cc121508f3a4062020-11-25T01:23:06ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-09-01810.3389/fcell.2020.578197578197Effects of Isorhamnetin in Human Amniotic Epithelial Stem Cells in vitro and Its Cardioprotective Effects in vivoKazuhiro Aonuma0Kazuhiro Aonuma1Farhana Ferdousi2Farhana Ferdousi3DongZhu Xu4DongZhu Xu5Kenichi Tominaga6Hiroko Isoda7Hiroko Isoda8Hiroko Isoda9Hiroko Isoda10School of Integrative and Global Majors (SIGMA), University of Tsukuba, Tsukuba, JapanAIST-University of Tsukuba Open Innovation Laboratory for Food and Medicinal Resource Engineering (FoodMed-OIL), AIST, University of Tsukuba, Tsukuba, JapanAIST-University of Tsukuba Open Innovation Laboratory for Food and Medicinal Resource Engineering (FoodMed-OIL), AIST, University of Tsukuba, Tsukuba, JapanAlliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba, Tsukuba, JapanAIST-University of Tsukuba Open Innovation Laboratory for Food and Medicinal Resource Engineering (FoodMed-OIL), AIST, University of Tsukuba, Tsukuba, JapanCardiovascular Division, Institute of Clinical Medicine, Faculty of Medicine, University of Tsukuba, Tsukuba, JapanAIST-University of Tsukuba Open Innovation Laboratory for Food and Medicinal Resource Engineering (FoodMed-OIL), AIST, University of Tsukuba, Tsukuba, JapanSchool of Integrative and Global Majors (SIGMA), University of Tsukuba, Tsukuba, JapanAIST-University of Tsukuba Open Innovation Laboratory for Food and Medicinal Resource Engineering (FoodMed-OIL), AIST, University of Tsukuba, Tsukuba, JapanAlliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba, Tsukuba, JapanFaculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, JapanCardiac hypertrophy and fibrosis are major pathophysiologic disorders that lead to serious cardiovascular diseases (CVDs), such as heart failure and arrhythmia. It is well known that transforming growth factor β (TGFβ) signaling pathways play a major role in the proliferation of cardiac hypertrophy and fibrosis, which is mainly stimulated by angiotensin II (AgII). This study aimed to investigate the cardioprotective potential of isorhamnetin (ISO) in human amniotic epithelial stem cells (hAESCs) through global gene expression analysis and to confirm its beneficial effects on cardiac hypertrophy and fibrosis in the AgII-induced in vivo model. In vitro, biological processes including TGFβ, collagen-related functions, and inflammatory processes were significantly suppressed in ISO pretreated hAESCs. In vivo, continuous AgII infusion using an osmotic pump induced significant pathological fibrosis and myocardial hypertrophy, which were remarkably suppressed by ISO pretreatment. ISO was found to reverse the enhanced TGFβ and Collagen type I alpha 1 mRNA expression induced by AgII exposure, which causes cardiovascular remodeling in ventricular tissue. These findings indicate that ISO could be a potential agent against cardiac hypertrophy and fibrosis.https://www.frontiersin.org/article/10.3389/fcell.2020.578197/fullhuman amniotic epithelial stem cellsdrug screeningisorhamnetincardiac fibrosisangiotensin IItranslational medicine |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kazuhiro Aonuma Kazuhiro Aonuma Farhana Ferdousi Farhana Ferdousi DongZhu Xu DongZhu Xu Kenichi Tominaga Hiroko Isoda Hiroko Isoda Hiroko Isoda Hiroko Isoda |
spellingShingle |
Kazuhiro Aonuma Kazuhiro Aonuma Farhana Ferdousi Farhana Ferdousi DongZhu Xu DongZhu Xu Kenichi Tominaga Hiroko Isoda Hiroko Isoda Hiroko Isoda Hiroko Isoda Effects of Isorhamnetin in Human Amniotic Epithelial Stem Cells in vitro and Its Cardioprotective Effects in vivo Frontiers in Cell and Developmental Biology human amniotic epithelial stem cells drug screening isorhamnetin cardiac fibrosis angiotensin II translational medicine |
author_facet |
Kazuhiro Aonuma Kazuhiro Aonuma Farhana Ferdousi Farhana Ferdousi DongZhu Xu DongZhu Xu Kenichi Tominaga Hiroko Isoda Hiroko Isoda Hiroko Isoda Hiroko Isoda |
author_sort |
Kazuhiro Aonuma |
title |
Effects of Isorhamnetin in Human Amniotic Epithelial Stem Cells in vitro and Its Cardioprotective Effects in vivo |
title_short |
Effects of Isorhamnetin in Human Amniotic Epithelial Stem Cells in vitro and Its Cardioprotective Effects in vivo |
title_full |
Effects of Isorhamnetin in Human Amniotic Epithelial Stem Cells in vitro and Its Cardioprotective Effects in vivo |
title_fullStr |
Effects of Isorhamnetin in Human Amniotic Epithelial Stem Cells in vitro and Its Cardioprotective Effects in vivo |
title_full_unstemmed |
Effects of Isorhamnetin in Human Amniotic Epithelial Stem Cells in vitro and Its Cardioprotective Effects in vivo |
title_sort |
effects of isorhamnetin in human amniotic epithelial stem cells in vitro and its cardioprotective effects in vivo |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2020-09-01 |
description |
Cardiac hypertrophy and fibrosis are major pathophysiologic disorders that lead to serious cardiovascular diseases (CVDs), such as heart failure and arrhythmia. It is well known that transforming growth factor β (TGFβ) signaling pathways play a major role in the proliferation of cardiac hypertrophy and fibrosis, which is mainly stimulated by angiotensin II (AgII). This study aimed to investigate the cardioprotective potential of isorhamnetin (ISO) in human amniotic epithelial stem cells (hAESCs) through global gene expression analysis and to confirm its beneficial effects on cardiac hypertrophy and fibrosis in the AgII-induced in vivo model. In vitro, biological processes including TGFβ, collagen-related functions, and inflammatory processes were significantly suppressed in ISO pretreated hAESCs. In vivo, continuous AgII infusion using an osmotic pump induced significant pathological fibrosis and myocardial hypertrophy, which were remarkably suppressed by ISO pretreatment. ISO was found to reverse the enhanced TGFβ and Collagen type I alpha 1 mRNA expression induced by AgII exposure, which causes cardiovascular remodeling in ventricular tissue. These findings indicate that ISO could be a potential agent against cardiac hypertrophy and fibrosis. |
topic |
human amniotic epithelial stem cells drug screening isorhamnetin cardiac fibrosis angiotensin II translational medicine |
url |
https://www.frontiersin.org/article/10.3389/fcell.2020.578197/full |
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