Assessing the Identity of Commercial Herbs From a Cambodian Market Using DNA Barcoding

In Cambodia, medicinal plants are often used to treat various illnesses. However, the identities of many medicinal plants remain unknown. In this study, we collected 50 types of traditional Cambodian medicinal plants that could not be identified by their appearance from a domestic market. We utilize...

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Main Authors: Xinyun Cui, Weijie Li, Jianhe Wei, Yaodong Qi, Rongtao Li, Yun Yang, Yuhua Shi, Xiangxiao Meng, Yaolei Mi, Theang Huot, Wei Sun, Xilong Zheng
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-03-01
Series:Frontiers in Pharmacology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fphar.2020.00244/full
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spelling doaj-3b02dbf1b8144c55a9c1e4b44119fc7c2020-11-25T02:17:49ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122020-03-011110.3389/fphar.2020.00244471137Assessing the Identity of Commercial Herbs From a Cambodian Market Using DNA BarcodingXinyun Cui0Weijie Li1Jianhe Wei2Jianhe Wei3Yaodong Qi4Rongtao Li5Yun Yang6Yuhua Shi7Xiangxiao Meng8Yaolei Mi9Theang Huot10Wei Sun11Xilong Zheng12Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, ChinaHainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, ChinaHainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaHainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, ChinaHainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaNational Center of Traditional Medicine, Ministry of Health of Cambodia, Phnom Penh, CambodiaKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaHainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, ChinaIn Cambodia, medicinal plants are often used to treat various illnesses. However, the identities of many medicinal plants remain unknown. In this study, we collected 50 types of traditional Cambodian medicinal plants that could not be identified by their appearance from a domestic market. We utilized the DNA barcoding technique, combined with the literature survey, to trace their identities. In the end, 33 species were identified at the species level and 7 species were identified at the genus level. The ethnopharmacological information of 33 medicinal plants was documented. The DNA barcoding technique is useful in the identification of medicinal plants with no previous information.https://www.frontiersin.org/article/10.3389/fphar.2020.00244/fullCambodiaherbDNA barcodingITS2psbA-trnH
collection DOAJ
language English
format Article
sources DOAJ
author Xinyun Cui
Weijie Li
Jianhe Wei
Jianhe Wei
Yaodong Qi
Rongtao Li
Yun Yang
Yuhua Shi
Xiangxiao Meng
Yaolei Mi
Theang Huot
Wei Sun
Xilong Zheng
spellingShingle Xinyun Cui
Weijie Li
Jianhe Wei
Jianhe Wei
Yaodong Qi
Rongtao Li
Yun Yang
Yuhua Shi
Xiangxiao Meng
Yaolei Mi
Theang Huot
Wei Sun
Xilong Zheng
Assessing the Identity of Commercial Herbs From a Cambodian Market Using DNA Barcoding
Frontiers in Pharmacology
Cambodia
herb
DNA barcoding
ITS2
psbA-trnH
author_facet Xinyun Cui
Weijie Li
Jianhe Wei
Jianhe Wei
Yaodong Qi
Rongtao Li
Yun Yang
Yuhua Shi
Xiangxiao Meng
Yaolei Mi
Theang Huot
Wei Sun
Xilong Zheng
author_sort Xinyun Cui
title Assessing the Identity of Commercial Herbs From a Cambodian Market Using DNA Barcoding
title_short Assessing the Identity of Commercial Herbs From a Cambodian Market Using DNA Barcoding
title_full Assessing the Identity of Commercial Herbs From a Cambodian Market Using DNA Barcoding
title_fullStr Assessing the Identity of Commercial Herbs From a Cambodian Market Using DNA Barcoding
title_full_unstemmed Assessing the Identity of Commercial Herbs From a Cambodian Market Using DNA Barcoding
title_sort assessing the identity of commercial herbs from a cambodian market using dna barcoding
publisher Frontiers Media S.A.
series Frontiers in Pharmacology
issn 1663-9812
publishDate 2020-03-01
description In Cambodia, medicinal plants are often used to treat various illnesses. However, the identities of many medicinal plants remain unknown. In this study, we collected 50 types of traditional Cambodian medicinal plants that could not be identified by their appearance from a domestic market. We utilized the DNA barcoding technique, combined with the literature survey, to trace their identities. In the end, 33 species were identified at the species level and 7 species were identified at the genus level. The ethnopharmacological information of 33 medicinal plants was documented. The DNA barcoding technique is useful in the identification of medicinal plants with no previous information.
topic Cambodia
herb
DNA barcoding
ITS2
psbA-trnH
url https://www.frontiersin.org/article/10.3389/fphar.2020.00244/full
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