Determination of Volatile Components from Live Water Lily Flowers by an Orthogonal-Array-Design-Assisted Trapping Cell
A convenient and easy-moving, modified, headspace solid-phase microextraction (HS-SPME) device was developed for monitoring a living plant’s volatile organic compounds (VOCs). It consisted of a polyethylene terephthalate (PET) bottle as a sampling chamber, and certain variables were consid...
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doaj-3b1f3961e266452c851fde598f428a702020-11-25T00:29:52ZengMDPI AGApplied Sciences2076-34172019-03-0197126910.3390/app9071269app9071269Determination of Volatile Components from Live Water Lily Flowers by an Orthogonal-Array-Design-Assisted Trapping CellFeng-Jie Tsai0Hsiao-Ju Liu1Ming-Yung Lee2Chih-Chien Lin3Department of Cosmetic Science, Providence University, 200, Sec. 7, Taiwan Boulevard, Shalu Dist., Taichung 43301, TaiwanDepartment of Cosmetic Science, Providence University, 200, Sec. 7, Taiwan Boulevard, Shalu Dist., Taichung 43301, TaiwanDepartment of Data Science and Big Data Analytics, Providence University, 200, Sec. 7, Taiwan Boulevard, Shalu Dist., Taichung 43301, TaiwanDepartment of Cosmetic Science, Providence University, 200, Sec. 7, Taiwan Boulevard, Shalu Dist., Taichung 43301, TaiwanA convenient and easy-moving, modified, headspace solid-phase microextraction (HS-SPME) device was developed for monitoring a living plant’s volatile organic compounds (VOCs). It consisted of a polyethylene terephthalate (PET) bottle as a sampling chamber, and certain variables were considered when using the HS-SPME device, including the material used and the fiber position, the direction of the airstream, and the distance between the sample and the fan. The results from varying those factors, generated by the orthogonal array design (OAD) method, were used to optimize the modified HS-SPME conditions. Based on the current literature regarding extracting fragrances by SPME, we selected polydimethylsiloxane/divinylbenzene (PDMS/DVB) and polydimethylsiloxane (PDMS) as the fiber materials. Using the OAD method, PDMS/DVB was found to be the better fiber material when it was parallel to the fan, and also when the airstream provided positive pressure to the sample with the fan near the sample. The device was used to sample biogenic volatile compounds emitted from fresh <i>Nymphaea caerulea</i> (water lily) flowers, followed by gas chromatography-mass spectrometry (GC-MS) analysis. For the method validation, under the optimum conditions, the calculated detection limit value of the model compound (butyl decanoate) was 0.14 ng on column, which was equal to 1.41 ppm for the injection. The relative standard deviations of the intra-day and inter-day precisions were 1.21% and 3.05%. Thirty-three compounds were separated and identified. The main components in the vapor phase of <i>N. caerulea</i> were benzyl acetate (10.4%), pentadecane (15.5%), 6,9-heptadecadiene (40.1%), and 8-heptadecene (15.3%).https://www.mdpi.com/2076-3417/9/7/1269<i>Nymphaea caerulea</i>water Lilyorthogonal array design (OAD)solid-phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS)volatile compoundslive plant |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Feng-Jie Tsai Hsiao-Ju Liu Ming-Yung Lee Chih-Chien Lin |
spellingShingle |
Feng-Jie Tsai Hsiao-Ju Liu Ming-Yung Lee Chih-Chien Lin Determination of Volatile Components from Live Water Lily Flowers by an Orthogonal-Array-Design-Assisted Trapping Cell Applied Sciences <i>Nymphaea caerulea</i> water Lily orthogonal array design (OAD) solid-phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) volatile compounds live plant |
author_facet |
Feng-Jie Tsai Hsiao-Ju Liu Ming-Yung Lee Chih-Chien Lin |
author_sort |
Feng-Jie Tsai |
title |
Determination of Volatile Components from Live Water Lily Flowers by an Orthogonal-Array-Design-Assisted Trapping Cell |
title_short |
Determination of Volatile Components from Live Water Lily Flowers by an Orthogonal-Array-Design-Assisted Trapping Cell |
title_full |
Determination of Volatile Components from Live Water Lily Flowers by an Orthogonal-Array-Design-Assisted Trapping Cell |
title_fullStr |
Determination of Volatile Components from Live Water Lily Flowers by an Orthogonal-Array-Design-Assisted Trapping Cell |
title_full_unstemmed |
Determination of Volatile Components from Live Water Lily Flowers by an Orthogonal-Array-Design-Assisted Trapping Cell |
title_sort |
determination of volatile components from live water lily flowers by an orthogonal-array-design-assisted trapping cell |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2019-03-01 |
description |
A convenient and easy-moving, modified, headspace solid-phase microextraction (HS-SPME) device was developed for monitoring a living plant’s volatile organic compounds (VOCs). It consisted of a polyethylene terephthalate (PET) bottle as a sampling chamber, and certain variables were considered when using the HS-SPME device, including the material used and the fiber position, the direction of the airstream, and the distance between the sample and the fan. The results from varying those factors, generated by the orthogonal array design (OAD) method, were used to optimize the modified HS-SPME conditions. Based on the current literature regarding extracting fragrances by SPME, we selected polydimethylsiloxane/divinylbenzene (PDMS/DVB) and polydimethylsiloxane (PDMS) as the fiber materials. Using the OAD method, PDMS/DVB was found to be the better fiber material when it was parallel to the fan, and also when the airstream provided positive pressure to the sample with the fan near the sample. The device was used to sample biogenic volatile compounds emitted from fresh <i>Nymphaea caerulea</i> (water lily) flowers, followed by gas chromatography-mass spectrometry (GC-MS) analysis. For the method validation, under the optimum conditions, the calculated detection limit value of the model compound (butyl decanoate) was 0.14 ng on column, which was equal to 1.41 ppm for the injection. The relative standard deviations of the intra-day and inter-day precisions were 1.21% and 3.05%. Thirty-three compounds were separated and identified. The main components in the vapor phase of <i>N. caerulea</i> were benzyl acetate (10.4%), pentadecane (15.5%), 6,9-heptadecadiene (40.1%), and 8-heptadecene (15.3%). |
topic |
<i>Nymphaea caerulea</i> water Lily orthogonal array design (OAD) solid-phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) volatile compounds live plant |
url |
https://www.mdpi.com/2076-3417/9/7/1269 |
work_keys_str_mv |
AT fengjietsai determinationofvolatilecomponentsfromlivewaterlilyflowersbyanorthogonalarraydesignassistedtrappingcell AT hsiaojuliu determinationofvolatilecomponentsfromlivewaterlilyflowersbyanorthogonalarraydesignassistedtrappingcell AT mingyunglee determinationofvolatilecomponentsfromlivewaterlilyflowersbyanorthogonalarraydesignassistedtrappingcell AT chihchienlin determinationofvolatilecomponentsfromlivewaterlilyflowersbyanorthogonalarraydesignassistedtrappingcell |
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