Kainic Acid and MPP+ Induce Upregulation of GLT-1 in Neuroblastoma and Glia Cells

Objective:Glutamate is the major excitatory transmitter in the brain. The excessive glutamate will lead to excitotoxicity. Glutamate transporter-1 (GLT-1) is the major transporter that performs 95% of the glutamate clearance contributing to normal neuronal function and preventing excitotoxicity. In...

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Bibliographic Details
Main Authors: Rabia UYARICI, Ceylan AK, Gizem DÖNMEZ YALÇIN
Format: Article
Language:English
Published: Galenos Publishing House 2021-04-01
Series:Bezmiâlem Science
Subjects:
Online Access: http://bezmialemscience.org/archives/archive-detail/article-preview/kainic-acid-and-mpp-nduce-upregulation-of-glt-1-in/47248
Description
Summary:Objective:Glutamate is the major excitatory transmitter in the brain. The excessive glutamate will lead to excitotoxicity. Glutamate transporter-1 (GLT-1) is the major transporter that performs 95% of the glutamate clearance contributing to normal neuronal function and preventing excitotoxicity. In this study, we investigated the effect of two toxins, kainic acid and MPP+ (1-methyl-4- phenylpyridinium), on GLT-1 expression and excitotoxicity in neuroblastoma and glia cells (immortalized human astrocytes).Methods:We treated neuroblastoma and glia cells with kainic acid and MPP+, applied MTT assay to measure the cell viability. We identified the mRNA and protein levels of GLT-1 and also analyzed released glutamate levels using glutamate assay.Results:The mRNA level of GLT-1 increased in neuroblastoma cells as a result of kainic acid or MPP+ treatment while the protein expression of GLT-1 increased in glia cells after the treatment with MPP+. Excess glutamate was found to be decreased after 12 h MPP+ treatment. However, this decrease was no more prominent with further MPP+ treatment.Conclusion:Our results show that GLT-1 levels are elevated as a result of kainic acid or MPP+ treatment as a survival mechanism to prevent excitotoxicity.
ISSN:2148-2373
2148-2373