Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization
The synthesis of polymer-coated gold nanoparticles with high colloidal stability is described, together with appropriate characterization techniques concerning the colloidal properties of the nanoparticles. Antibodies against vascular endothelial growth factor (VEGF) are conjugated to the surface of...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2015-08-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2079-4991/5/3/1297 |
| id |
doaj-3b67b7507bd24511b128c02dc60e96b8 |
|---|---|
| record_format |
Article |
| spelling |
doaj-3b67b7507bd24511b128c02dc60e96b82020-11-24T23:24:00ZengMDPI AGNanomaterials2079-49912015-08-01531297131610.3390/nano5031297nano5031297Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and CharacterizationGamze Tan0Karsten Kantner1Qian Zhang2Mahmoud G. Soliman3Pablo del Pino4Wolfgang J. Parak5Mehmet A. Onur6Daniel Valdeperez7Joanna Rejman8Beatriz Pelaz9Faculty of Science and Letters, Department of Biology, Aksaray University, Aksaray 68100,TurkeyPhilipp University of Marburg, Marburg 35001, GermanyPhilipp University of Marburg, Marburg 35001, GermanyPhilipp University of Marburg, Marburg 35001, GermanyCentro de Investigación Cooperativa Biomagune, San Sebastián 20001, SpainPhilipp University of Marburg, Marburg 35001, GermanyFaculty of Science, Department of Biology, Hacettepe University, Ankara 06800, TurkeyPhilipp University of Marburg, Marburg 35001, GermanyPhilipp University of Marburg, Marburg 35001, GermanyPhilipp University of Marburg, Marburg 35001, GermanyThe synthesis of polymer-coated gold nanoparticles with high colloidal stability is described, together with appropriate characterization techniques concerning the colloidal properties of the nanoparticles. Antibodies against vascular endothelial growth factor (VEGF) are conjugated to the surface of the nanoparticles. Antibody attachment is probed by different techniques, giving a guideline about the characterization of such conjugates. The effect of the nanoparticles on human adenocarcinoma alveolar basal epithelial cells (A549) and human umbilical vein endothelial cells (HUVECs) is probed in terms of internalization and viability assays.http://www.mdpi.com/2079-4991/5/3/1297gold nanoparticlesbioconjugationnanoparticle characterizationtoxicitynanoparticle-cell interactioncellular uptakeVEGF |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Gamze Tan Karsten Kantner Qian Zhang Mahmoud G. Soliman Pablo del Pino Wolfgang J. Parak Mehmet A. Onur Daniel Valdeperez Joanna Rejman Beatriz Pelaz |
| spellingShingle |
Gamze Tan Karsten Kantner Qian Zhang Mahmoud G. Soliman Pablo del Pino Wolfgang J. Parak Mehmet A. Onur Daniel Valdeperez Joanna Rejman Beatriz Pelaz Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization Nanomaterials gold nanoparticles bioconjugation nanoparticle characterization toxicity nanoparticle-cell interaction cellular uptake VEGF |
| author_facet |
Gamze Tan Karsten Kantner Qian Zhang Mahmoud G. Soliman Pablo del Pino Wolfgang J. Parak Mehmet A. Onur Daniel Valdeperez Joanna Rejman Beatriz Pelaz |
| author_sort |
Gamze Tan |
| title |
Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization |
| title_short |
Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization |
| title_full |
Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization |
| title_fullStr |
Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization |
| title_full_unstemmed |
Conjugation of Polymer-Coated Gold Nanoparticles with Antibodies—Synthesis and Characterization |
| title_sort |
conjugation of polymer-coated gold nanoparticles with antibodies—synthesis and characterization |
| publisher |
MDPI AG |
| series |
Nanomaterials |
| issn |
2079-4991 |
| publishDate |
2015-08-01 |
| description |
The synthesis of polymer-coated gold nanoparticles with high colloidal stability is described, together with appropriate characterization techniques concerning the colloidal properties of the nanoparticles. Antibodies against vascular endothelial growth factor (VEGF) are conjugated to the surface of the nanoparticles. Antibody attachment is probed by different techniques, giving a guideline about the characterization of such conjugates. The effect of the nanoparticles on human adenocarcinoma alveolar basal epithelial cells (A549) and human umbilical vein endothelial cells (HUVECs) is probed in terms of internalization and viability assays. |
| topic |
gold nanoparticles bioconjugation nanoparticle characterization toxicity nanoparticle-cell interaction cellular uptake VEGF |
| url |
http://www.mdpi.com/2079-4991/5/3/1297 |
| work_keys_str_mv |
AT gamzetan conjugationofpolymercoatedgoldnanoparticleswithantibodiessynthesisandcharacterization AT karstenkantner conjugationofpolymercoatedgoldnanoparticleswithantibodiessynthesisandcharacterization AT qianzhang conjugationofpolymercoatedgoldnanoparticleswithantibodiessynthesisandcharacterization AT mahmoudgsoliman conjugationofpolymercoatedgoldnanoparticleswithantibodiessynthesisandcharacterization AT pablodelpino conjugationofpolymercoatedgoldnanoparticleswithantibodiessynthesisandcharacterization AT wolfgangjparak conjugationofpolymercoatedgoldnanoparticleswithantibodiessynthesisandcharacterization AT mehmetaonur conjugationofpolymercoatedgoldnanoparticleswithantibodiessynthesisandcharacterization AT danielvaldeperez conjugationofpolymercoatedgoldnanoparticleswithantibodiessynthesisandcharacterization AT joannarejman conjugationofpolymercoatedgoldnanoparticleswithantibodiessynthesisandcharacterization AT beatrizpelaz conjugationofpolymercoatedgoldnanoparticleswithantibodiessynthesisandcharacterization |
| _version_ |
1725562340789714944 |