Polarity directed optimization of phytochemical and in vitro biological potential of an indigenous folklore: Quercus dilatata Lindl. ex Royle

Abstract Background Plants have served either as a natural templates for the development of new chemicals or a phytomedicine since antiquity. Therefore, the present study was aimed to appraise the polarity directed antioxidant, cytotoxic, protein kinase inhibitory, antileishmanial and glucose modula...

Full description

Bibliographic Details
Main Authors: Madiha Ahmed, Humaira Fatima, Muhammad Qasim, Bilquees Gul, Ihsan-ul-Haq
Format: Article
Language:English
Published: BMC 2017-08-01
Series:BMC Complementary and Alternative Medicine
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12906-017-1894-x
id doaj-3b758039a2b542bb87cf2e0ace6eb1fd
record_format Article
spelling doaj-3b758039a2b542bb87cf2e0ace6eb1fd2020-11-25T02:01:57ZengBMCBMC Complementary and Alternative Medicine1472-68822017-08-0117111610.1186/s12906-017-1894-xPolarity directed optimization of phytochemical and in vitro biological potential of an indigenous folklore: Quercus dilatata Lindl. ex RoyleMadiha Ahmed0Humaira Fatima1Muhammad Qasim2Bilquees Gul3Ihsan-ul-Haq4Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam UniversityDepartment of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam UniversityInstitute of Sustainable Halophyte Utilization, University of KarachiInstitute of Sustainable Halophyte Utilization, University of KarachiDepartment of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam UniversityAbstract Background Plants have served either as a natural templates for the development of new chemicals or a phytomedicine since antiquity. Therefore, the present study was aimed to appraise the polarity directed antioxidant, cytotoxic, protein kinase inhibitory, antileishmanial and glucose modulatory attributes of a Himalayan medicinal plant- Quercus dilatata. Methods Total phenolic and flavonoid contents were determined colorimetrically and various polyphenols were identified by RP-HPLC analysis. Brine shrimp lethality, SRB and MTT assays were employed to test cytotoxicity against Artemia salina and human cancer cell lines respectively. Antileishmanial activity was determined using standard MTT protocol. Glucose modulation was assessed by α-amylase inhibition assay while disc diffusion assay was used to establish protein kinase inhibitory and antifungal spectrum. Results Among 14 extracts of aerial parts, distilled water-acetone extract demonstrated maximum extract recovery (10.52% w/w), phenolic content (21.37 ± 0.21 μg GAE/mg dry weight (DW)), total antioxidant capacity (4.81 ± 0.98 μg AAE/mg DW) and reducing power potential (20.03 ± 2.4 μg/mg DW). On the other hand, Distilled water extract proficiently extracted flavonoid content (4.78 ± 0.51 μg QE/mg DW). RP-HPLC analysis revealed the presence of significant amounts of phenolic metabolites (0.049 to 15.336 μg/mg extract) including, pyrocatechol, gallic acid, catechin, chlorogenic acid, p-coumaric acid, ferulic acid and quercetin. Highest free radical scavenging capacity was found in Methanol-Ethyl acetate extract (IC50 8.1 ± 0.5 μg/ml). In the brine shrimp toxicity assay, most of the tested extracts (57%) showed high cytotoxicity. Among these, Chloroform-Methanol extract had highest cytotoxicity against THP-1 cell line (IC50 3.88 ± 0.53 μg/ml). About 50% of the extracts were found to be moderately antiproliferative against Hep G2 cell line. Methanol extract exhibited considerable protein kinase inhibitory activity against Streptomyces 85E strain (28 ± 0.35 mm bald phenotype at 100 μg/disc; MIC = 12.5 μg/ disc) while, Chloroform extract displayed maximum antidiabetic activity (α-amylase inhibition of 21.61 ± 1.53% at 200 μg/ml concentration). The highest antileishmanial potential was found in Ethyl acetate-Acetone extract (12.91 ± 0.02% at 100 μg/ml concentration), while, Q. dilatata extracts also showed a moderate antifungal activity. Conclusion This study proposes that multiple-solvent system is a crucial variable to elucidate pharmacological potential of Q. dilatata and the results of the present findings prospects its potential as a resource for the discovery of novel anticancer, antidiabetic, antileishmanial and antioxidant agents.http://link.springer.com/article/10.1186/s12906-017-1894-xAntileishmanialAntioxidantCytotoxicityHep G2Protein kinase inhibitionTHP-1 leukemia cell line
collection DOAJ
language English
format Article
sources DOAJ
author Madiha Ahmed
Humaira Fatima
Muhammad Qasim
Bilquees Gul
Ihsan-ul-Haq
spellingShingle Madiha Ahmed
Humaira Fatima
Muhammad Qasim
Bilquees Gul
Ihsan-ul-Haq
Polarity directed optimization of phytochemical and in vitro biological potential of an indigenous folklore: Quercus dilatata Lindl. ex Royle
BMC Complementary and Alternative Medicine
Antileishmanial
Antioxidant
Cytotoxicity
Hep G2
Protein kinase inhibition
THP-1 leukemia cell line
author_facet Madiha Ahmed
Humaira Fatima
Muhammad Qasim
Bilquees Gul
Ihsan-ul-Haq
author_sort Madiha Ahmed
title Polarity directed optimization of phytochemical and in vitro biological potential of an indigenous folklore: Quercus dilatata Lindl. ex Royle
title_short Polarity directed optimization of phytochemical and in vitro biological potential of an indigenous folklore: Quercus dilatata Lindl. ex Royle
title_full Polarity directed optimization of phytochemical and in vitro biological potential of an indigenous folklore: Quercus dilatata Lindl. ex Royle
title_fullStr Polarity directed optimization of phytochemical and in vitro biological potential of an indigenous folklore: Quercus dilatata Lindl. ex Royle
title_full_unstemmed Polarity directed optimization of phytochemical and in vitro biological potential of an indigenous folklore: Quercus dilatata Lindl. ex Royle
title_sort polarity directed optimization of phytochemical and in vitro biological potential of an indigenous folklore: quercus dilatata lindl. ex royle
publisher BMC
series BMC Complementary and Alternative Medicine
issn 1472-6882
publishDate 2017-08-01
description Abstract Background Plants have served either as a natural templates for the development of new chemicals or a phytomedicine since antiquity. Therefore, the present study was aimed to appraise the polarity directed antioxidant, cytotoxic, protein kinase inhibitory, antileishmanial and glucose modulatory attributes of a Himalayan medicinal plant- Quercus dilatata. Methods Total phenolic and flavonoid contents were determined colorimetrically and various polyphenols were identified by RP-HPLC analysis. Brine shrimp lethality, SRB and MTT assays were employed to test cytotoxicity against Artemia salina and human cancer cell lines respectively. Antileishmanial activity was determined using standard MTT protocol. Glucose modulation was assessed by α-amylase inhibition assay while disc diffusion assay was used to establish protein kinase inhibitory and antifungal spectrum. Results Among 14 extracts of aerial parts, distilled water-acetone extract demonstrated maximum extract recovery (10.52% w/w), phenolic content (21.37 ± 0.21 μg GAE/mg dry weight (DW)), total antioxidant capacity (4.81 ± 0.98 μg AAE/mg DW) and reducing power potential (20.03 ± 2.4 μg/mg DW). On the other hand, Distilled water extract proficiently extracted flavonoid content (4.78 ± 0.51 μg QE/mg DW). RP-HPLC analysis revealed the presence of significant amounts of phenolic metabolites (0.049 to 15.336 μg/mg extract) including, pyrocatechol, gallic acid, catechin, chlorogenic acid, p-coumaric acid, ferulic acid and quercetin. Highest free radical scavenging capacity was found in Methanol-Ethyl acetate extract (IC50 8.1 ± 0.5 μg/ml). In the brine shrimp toxicity assay, most of the tested extracts (57%) showed high cytotoxicity. Among these, Chloroform-Methanol extract had highest cytotoxicity against THP-1 cell line (IC50 3.88 ± 0.53 μg/ml). About 50% of the extracts were found to be moderately antiproliferative against Hep G2 cell line. Methanol extract exhibited considerable protein kinase inhibitory activity against Streptomyces 85E strain (28 ± 0.35 mm bald phenotype at 100 μg/disc; MIC = 12.5 μg/ disc) while, Chloroform extract displayed maximum antidiabetic activity (α-amylase inhibition of 21.61 ± 1.53% at 200 μg/ml concentration). The highest antileishmanial potential was found in Ethyl acetate-Acetone extract (12.91 ± 0.02% at 100 μg/ml concentration), while, Q. dilatata extracts also showed a moderate antifungal activity. Conclusion This study proposes that multiple-solvent system is a crucial variable to elucidate pharmacological potential of Q. dilatata and the results of the present findings prospects its potential as a resource for the discovery of novel anticancer, antidiabetic, antileishmanial and antioxidant agents.
topic Antileishmanial
Antioxidant
Cytotoxicity
Hep G2
Protein kinase inhibition
THP-1 leukemia cell line
url http://link.springer.com/article/10.1186/s12906-017-1894-x
work_keys_str_mv AT madihaahmed polaritydirectedoptimizationofphytochemicalandinvitrobiologicalpotentialofanindigenousfolklorequercusdilatatalindlexroyle
AT humairafatima polaritydirectedoptimizationofphytochemicalandinvitrobiologicalpotentialofanindigenousfolklorequercusdilatatalindlexroyle
AT muhammadqasim polaritydirectedoptimizationofphytochemicalandinvitrobiologicalpotentialofanindigenousfolklorequercusdilatatalindlexroyle
AT bilqueesgul polaritydirectedoptimizationofphytochemicalandinvitrobiologicalpotentialofanindigenousfolklorequercusdilatatalindlexroyle
AT ihsanulhaq polaritydirectedoptimizationofphytochemicalandinvitrobiologicalpotentialofanindigenousfolklorequercusdilatatalindlexroyle
_version_ 1724954904568528896