Anti-colorectal cancer effects of scutellarin revealed by genomic and proteomic analysis
Abstract Background Colorectal cancer, one of the most common digestive tumors with high mortality and morbidity worldwide, currently lacks effective therapies available to improve the prognosis. This study was aimed to investigate the potency of Scutellarin against colorectal cancers, and explore t...
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doaj-3b92ba4190214eb684f8deede875eae42020-11-25T02:40:35ZengBMCChinese Medicine1749-85462020-03-0115111510.1186/s13020-020-00307-zAnti-colorectal cancer effects of scutellarin revealed by genomic and proteomic analysisLiu-Lin Xiong0Ruo-Lan Du1Lu-Lu Xue2Ya Jiang3Jin Huang4Li Chen5Jia Liu6Ting-Hua Wang7Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan UniversityInstitute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan UniversityAnimal Zoology Department, Institute of Neuroscience, Kunming Medical UniversityAnimal Zoology Department, Institute of Neuroscience, Kunming Medical UniversityAnimal Zoology Department, Institute of Neuroscience, Kunming Medical UniversityInstitute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan UniversityAnimal Zoology Department, Institute of Neuroscience, Kunming Medical UniversityInstitute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan UniversityAbstract Background Colorectal cancer, one of the most common digestive tumors with high mortality and morbidity worldwide, currently lacks effective therapies available to improve the prognosis. This study was aimed to investigate the potency of Scutellarin against colorectal cancers, and explore the related mechanism via genomic and proteomic analysis. Methods Cell counting kit-8 assay was employed to detect the viability of HCT-116 and RKO cell lines treated with Scutellarin. The apoptosis of HCT-116 and RKO cells after Scutellarin administration was determined by TUNEL staining and Caspase 3/7 activity. Cell cycle was detected by flow cytometry analysis. The wound healing and transwell invasion test detected the role of Scutellarin in migration and invasion of HCT-116 and RKO cells. Meanwhile, the energy metabolism and growth of tumor tissues in vivo at day 28 were observed by PET-CT after Scutellarin administration with 50 mg/kg, 100 mg/kg and 300 mg/kg into 4-week-old nude mice. Blood routine and liver functions were also detected to evaluate the side effect of Scutellarin. Furthermore, the disease and function classifications which the differentially expressed genes and proteins involved after Scutellarin treatment were determined by genomic and proteomic analysis respectively. Results The Scutellarin inhibited the migration and increased apoptosis of HCT-116 and RKO cell lines. Besides, Scutellarin treatment substantially decreased the growth and volume of colorectal tumors in nude mice without side effects on the blood routine and liver function. The differentially expressed genes in RKO cells after Scutellarin administration were mainly enriched in cell death and survival, organismal injury and abnormalities, and cancer. In addition, forty-seven upregulated and twenty-nine downregulated proteins were identified. Functional clustering analysis exhibited enriched biological processes, cellular components, molecular functions and related pathways of these proteins in cellular metabolic. Then protein–protein interactions analysis showed the regulatory relationship among these differentially expressed proteins. Conclusions Taken together, the present findings revealed that Scutellarin exerted significant antitumor effect with no side effects in the blood and liver by regulating various important molecules in tumor proliferation, apoptosis and metastasis.http://link.springer.com/article/10.1186/s13020-020-00307-zColorectal cancerScutellarinAntitumor effectGenomics, proteomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liu-Lin Xiong Ruo-Lan Du Lu-Lu Xue Ya Jiang Jin Huang Li Chen Jia Liu Ting-Hua Wang |
spellingShingle |
Liu-Lin Xiong Ruo-Lan Du Lu-Lu Xue Ya Jiang Jin Huang Li Chen Jia Liu Ting-Hua Wang Anti-colorectal cancer effects of scutellarin revealed by genomic and proteomic analysis Chinese Medicine Colorectal cancer Scutellarin Antitumor effect Genomics, proteomics |
author_facet |
Liu-Lin Xiong Ruo-Lan Du Lu-Lu Xue Ya Jiang Jin Huang Li Chen Jia Liu Ting-Hua Wang |
author_sort |
Liu-Lin Xiong |
title |
Anti-colorectal cancer effects of scutellarin revealed by genomic and proteomic analysis |
title_short |
Anti-colorectal cancer effects of scutellarin revealed by genomic and proteomic analysis |
title_full |
Anti-colorectal cancer effects of scutellarin revealed by genomic and proteomic analysis |
title_fullStr |
Anti-colorectal cancer effects of scutellarin revealed by genomic and proteomic analysis |
title_full_unstemmed |
Anti-colorectal cancer effects of scutellarin revealed by genomic and proteomic analysis |
title_sort |
anti-colorectal cancer effects of scutellarin revealed by genomic and proteomic analysis |
publisher |
BMC |
series |
Chinese Medicine |
issn |
1749-8546 |
publishDate |
2020-03-01 |
description |
Abstract Background Colorectal cancer, one of the most common digestive tumors with high mortality and morbidity worldwide, currently lacks effective therapies available to improve the prognosis. This study was aimed to investigate the potency of Scutellarin against colorectal cancers, and explore the related mechanism via genomic and proteomic analysis. Methods Cell counting kit-8 assay was employed to detect the viability of HCT-116 and RKO cell lines treated with Scutellarin. The apoptosis of HCT-116 and RKO cells after Scutellarin administration was determined by TUNEL staining and Caspase 3/7 activity. Cell cycle was detected by flow cytometry analysis. The wound healing and transwell invasion test detected the role of Scutellarin in migration and invasion of HCT-116 and RKO cells. Meanwhile, the energy metabolism and growth of tumor tissues in vivo at day 28 were observed by PET-CT after Scutellarin administration with 50 mg/kg, 100 mg/kg and 300 mg/kg into 4-week-old nude mice. Blood routine and liver functions were also detected to evaluate the side effect of Scutellarin. Furthermore, the disease and function classifications which the differentially expressed genes and proteins involved after Scutellarin treatment were determined by genomic and proteomic analysis respectively. Results The Scutellarin inhibited the migration and increased apoptosis of HCT-116 and RKO cell lines. Besides, Scutellarin treatment substantially decreased the growth and volume of colorectal tumors in nude mice without side effects on the blood routine and liver function. The differentially expressed genes in RKO cells after Scutellarin administration were mainly enriched in cell death and survival, organismal injury and abnormalities, and cancer. In addition, forty-seven upregulated and twenty-nine downregulated proteins were identified. Functional clustering analysis exhibited enriched biological processes, cellular components, molecular functions and related pathways of these proteins in cellular metabolic. Then protein–protein interactions analysis showed the regulatory relationship among these differentially expressed proteins. Conclusions Taken together, the present findings revealed that Scutellarin exerted significant antitumor effect with no side effects in the blood and liver by regulating various important molecules in tumor proliferation, apoptosis and metastasis. |
topic |
Colorectal cancer Scutellarin Antitumor effect Genomics, proteomics |
url |
http://link.springer.com/article/10.1186/s13020-020-00307-z |
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