The Effects of Nigella sativa Hydro-alcoholic Extract andHoney on Lipid Profile and Indices of Insulin Resistance inPolycystic Ovarian Syndrome Wistar Rat Model

• Main Authors: Seiyedeh Narjes Naseran, Mokhtar Mokhtari, Mahmood Abedinzade, MehrdadShariati
• Format: Article
• Language: English
• Published: Amber Publication 2018-07-01
• Series: Journal of Research in Medical and Dental Science
• Subjects: Nigella sativa; Honey; Polycystic ovarian syndrome; Insulin resistance; Lipid profile; Wistar rat
• Online Access: https://www.jrmds.in/articles/the-effects-of-nigella-sativa-hydroalcoholic-extract-and-honey-on-lipid-profile-and-indices-of-insulin-resistance-in-polycystic-ov.pdf

Background: Polycystic ovary syndrome (PCOS) is a common complex endocrine and metabolic disorder. The cause of PCOSis still unknown and may be due to several factors. The aim of the present study was to evaluate the combined effect ofNigella sativa hydro-alcoholic extract and honey on triglyceride (TG), total cholesterol (TC), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C), glucose and insulin in PCOS Wistar rat model.Materials and Methods: 72 adult Wistar rats, weighting 200-220 g were divided randomly into 9 groups (n=8): includingcontrol (intact), Sham (letrozole solvent), control PCOS and 6 experimental PCOS groups. Rats were treated with letrozolefor 21 days to induce PCOS. In experimental groups, PCOS rats were treated with 2 doses of honey (1200 and 2400 mg/k), 2doses of Nigella sativa extract (300 mg/k and 600 mg/k) and 2 doses of combination of Nigella sativa extract and honey bythe gavage for 28 days. After harvesting blood serum, lipid profile, glucose and insulin were measured by ELISA method.SPSS software version 16 was used for data analysis.Results: Serological analysis showed lower TG, TC, LDL, Glucose and insulin in all experimental groups. TG, TC, LDL, Glucoseand insulin, particularly at dose 2 of combination of Nigella sativa extract and Honey was significantly decreased (p ≤ 0.05)as compared with control PCOS group. HDL level was significantly increase in dose2 of combination of N. sativa extract andHoney groups.Conclusion: Combination of Nigella sativa extract and Honey improves lipid profile and the sensitivity to insulin in patientswith PCOS.