Gastric Helicobacter pylori infection perturbs human oral microbiota

• Main Authors: Eng-Guan Chua, Ju-Yee Chong, Binit Lamichhane, K. Mary Webberley, Barry J. Marshall, Michael J. Wise, Chin-Yen Tay
• Format: Article
• Language: English
• Published: PeerJ Inc. 2019-01-01
• Series: PeerJ
• Subjects: Oral microbiome; Urea breath test; Helicobacter pylori; 16S rRNA sequencing; Metagenomics
• Online Access: https://peerj.com/articles/6336.pdf

Background We investigated the effects of gastric Helicobacter pylori infection on the daytime and overnight human oral microbiota. Methods Twenty four volunteers were recruited. Ten tested positive for H. pylori infection by the Carbon-14 Urea Breath Test, and the rest were negative. Two oral swabs were collected: one immediately after waking up in the morning and before brushing teeth, and another in the evening before teeth-brushing. DNA extract acquired from each swab was subjected to Illumina sequencing of 16S rRNA gene amplicons. The microbial abundance and composition were analysed in relation to H. pylori infection status. Results Helicobacter pylori-positive individuals had significant changes in the alpha and beta diversities in the daytime samples in comparison to those who were H. pylori negative. To identify which taxa could be significantly affected within the cohorts in the daytime, we employed the LEfSe method. When compared against UBT-negative samples, significantly higher abundances were detected in both Pseudomonas and Roseomonas, while Fusobacterium, Solobacterium, Haemophilus and Streptococcus were significantly decreased in the UBT-positive samples. Discussion Our data demonstrated that H. pylori infection affects the human daytime oral microbiota. The hitherto undocumented changes of several bacterial genera due to H. pylori infection require more studies to examine their potential health effects on affected individuals.