Forskolin Inhibits Lipopolysaccharide-Induced Modulation of MCP-1 and GPR120 in 3T3-L1 Adipocytes through an Inhibition of NFκB
In an obese state, Toll-like receptor-4 (TLR-4) upregulates proinflammatory adipokines secretion including monocyte chemotactic protein-1 (MCP-1) in adipose tissue. In contrast, G-protein coupled receptor 120 (GPR120) mediates antiobesity effects. The aim of this study was to determine the signaling...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2016-01-01
|
Series: | Mediators of Inflammation |
Online Access: | http://dx.doi.org/10.1155/2016/1431789 |
id |
doaj-3bd75f715a604874a5da171774ac3191 |
---|---|
record_format |
Article |
spelling |
doaj-3bd75f715a604874a5da171774ac31912020-11-24T22:08:46ZengHindawi LimitedMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/14317891431789Forskolin Inhibits Lipopolysaccharide-Induced Modulation of MCP-1 and GPR120 in 3T3-L1 Adipocytes through an Inhibition of NFκBJeanne Durendale Chiadak0Tatjana Arsenijevic1Kevin Verstrepen2Françoise Gregoire3Nargis Bolaky4Valérie Delforge5Véronique Flamand6Jason Perret7Christine Delporte8Laboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles, Brussels, BelgiumLaboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles, Brussels, BelgiumLaboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles, Brussels, BelgiumLaboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles, Brussels, BelgiumLaboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles, Brussels, BelgiumLaboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles, Brussels, BelgiumInstitute for Medical Immunology, Faculty of Medicine, Université Libre de Bruxelles, Gosselies, BelgiumLaboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles, Brussels, BelgiumLaboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles, Brussels, BelgiumIn an obese state, Toll-like receptor-4 (TLR-4) upregulates proinflammatory adipokines secretion including monocyte chemotactic protein-1 (MCP-1) in adipose tissue. In contrast, G-protein coupled receptor 120 (GPR120) mediates antiobesity effects. The aim of this study was to determine the signaling pathway by which Forskolin (FK), a cyclic adenosine monophosphate- (cAMP-) promoting agent causing positive changes in body composition in overweight and obese adult men, affects MCP-1 and GPR120 expression during an inflammatory response induced by lipopolysaccharide (LPS) in adipocytes, such as in an obese state. 3T3-L1 cells differentiated into adipocytes (DC) were stimulated with LPS in the absence or presence of FK and inhibitors of TLR-4 and inhibitor of kappa B (IκBα). In DC, LPS increased MCP-1, TLR-4, and nuclear factor-κB1 (NFκB1) mRNA levels, whereas it decreased GPR120 mRNA levels. In DC, FK inhibited the LPS-induced increase in MCP-1, TLR-4, and NFκB1 mRNA levels and the LPS-induced decrease in GPR120 mRNA. BAY11-7082 and CLI-095 abolished these LPS-induced effects. In conclusion, FK inhibits LPS-induced increase in MCP-1 mRNA levels and decrease in GPR120 mRNA levels in adipocytes and may be a potential treatment for inflammation in obesity. Furthermore, TLR-4-induced activation of NFκB may be involved in the LPS-induced regulation of these genes.http://dx.doi.org/10.1155/2016/1431789 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jeanne Durendale Chiadak Tatjana Arsenijevic Kevin Verstrepen Françoise Gregoire Nargis Bolaky Valérie Delforge Véronique Flamand Jason Perret Christine Delporte |
spellingShingle |
Jeanne Durendale Chiadak Tatjana Arsenijevic Kevin Verstrepen Françoise Gregoire Nargis Bolaky Valérie Delforge Véronique Flamand Jason Perret Christine Delporte Forskolin Inhibits Lipopolysaccharide-Induced Modulation of MCP-1 and GPR120 in 3T3-L1 Adipocytes through an Inhibition of NFκB Mediators of Inflammation |
author_facet |
Jeanne Durendale Chiadak Tatjana Arsenijevic Kevin Verstrepen Françoise Gregoire Nargis Bolaky Valérie Delforge Véronique Flamand Jason Perret Christine Delporte |
author_sort |
Jeanne Durendale Chiadak |
title |
Forskolin Inhibits Lipopolysaccharide-Induced Modulation of MCP-1 and GPR120 in 3T3-L1 Adipocytes through an Inhibition of NFκB |
title_short |
Forskolin Inhibits Lipopolysaccharide-Induced Modulation of MCP-1 and GPR120 in 3T3-L1 Adipocytes through an Inhibition of NFκB |
title_full |
Forskolin Inhibits Lipopolysaccharide-Induced Modulation of MCP-1 and GPR120 in 3T3-L1 Adipocytes through an Inhibition of NFκB |
title_fullStr |
Forskolin Inhibits Lipopolysaccharide-Induced Modulation of MCP-1 and GPR120 in 3T3-L1 Adipocytes through an Inhibition of NFκB |
title_full_unstemmed |
Forskolin Inhibits Lipopolysaccharide-Induced Modulation of MCP-1 and GPR120 in 3T3-L1 Adipocytes through an Inhibition of NFκB |
title_sort |
forskolin inhibits lipopolysaccharide-induced modulation of mcp-1 and gpr120 in 3t3-l1 adipocytes through an inhibition of nfκb |
publisher |
Hindawi Limited |
series |
Mediators of Inflammation |
issn |
0962-9351 1466-1861 |
publishDate |
2016-01-01 |
description |
In an obese state, Toll-like receptor-4 (TLR-4) upregulates proinflammatory adipokines secretion including monocyte chemotactic protein-1 (MCP-1) in adipose tissue. In contrast, G-protein coupled receptor 120 (GPR120) mediates antiobesity effects. The aim of this study was to determine the signaling pathway by which Forskolin (FK), a cyclic adenosine monophosphate- (cAMP-) promoting agent causing positive changes in body composition in overweight and obese adult men, affects MCP-1 and GPR120 expression during an inflammatory response induced by lipopolysaccharide (LPS) in adipocytes, such as in an obese state. 3T3-L1 cells differentiated into adipocytes (DC) were stimulated with LPS in the absence or presence of FK and inhibitors of TLR-4 and inhibitor of kappa B (IκBα). In DC, LPS increased MCP-1, TLR-4, and nuclear factor-κB1 (NFκB1) mRNA levels, whereas it decreased GPR120 mRNA levels. In DC, FK inhibited the LPS-induced increase in MCP-1, TLR-4, and NFκB1 mRNA levels and the LPS-induced decrease in GPR120 mRNA. BAY11-7082 and CLI-095 abolished these LPS-induced effects. In conclusion, FK inhibits LPS-induced increase in MCP-1 mRNA levels and decrease in GPR120 mRNA levels in adipocytes and may be a potential treatment for inflammation in obesity. Furthermore, TLR-4-induced activation of NFκB may be involved in the LPS-induced regulation of these genes. |
url |
http://dx.doi.org/10.1155/2016/1431789 |
work_keys_str_mv |
AT jeannedurendalechiadak forskolininhibitslipopolysaccharideinducedmodulationofmcp1andgpr120in3t3l1adipocytesthroughaninhibitionofnfkb AT tatjanaarsenijevic forskolininhibitslipopolysaccharideinducedmodulationofmcp1andgpr120in3t3l1adipocytesthroughaninhibitionofnfkb AT kevinverstrepen forskolininhibitslipopolysaccharideinducedmodulationofmcp1andgpr120in3t3l1adipocytesthroughaninhibitionofnfkb AT francoisegregoire forskolininhibitslipopolysaccharideinducedmodulationofmcp1andgpr120in3t3l1adipocytesthroughaninhibitionofnfkb AT nargisbolaky forskolininhibitslipopolysaccharideinducedmodulationofmcp1andgpr120in3t3l1adipocytesthroughaninhibitionofnfkb AT valeriedelforge forskolininhibitslipopolysaccharideinducedmodulationofmcp1andgpr120in3t3l1adipocytesthroughaninhibitionofnfkb AT veroniqueflamand forskolininhibitslipopolysaccharideinducedmodulationofmcp1andgpr120in3t3l1adipocytesthroughaninhibitionofnfkb AT jasonperret forskolininhibitslipopolysaccharideinducedmodulationofmcp1andgpr120in3t3l1adipocytesthroughaninhibitionofnfkb AT christinedelporte forskolininhibitslipopolysaccharideinducedmodulationofmcp1andgpr120in3t3l1adipocytesthroughaninhibitionofnfkb |
_version_ |
1725814762542989312 |