Transcriptional insights into the pyramided resistance to rice bacterial blight
Abstract The pyramiding of resistance (R) genes provides broad-spectrum and durable resistance to plant diseases. However, the genetic basis for bacterial blight (BB) resistance remains unclear. The BB R gene pyramided line IRBB54, which expresses xa5 and Xa21, possessed a higher level of resistance...
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doaj-3bee81b176f04b19a5ce2cd6cae4a7eb2020-12-08T04:32:35ZengNature Publishing GroupScientific Reports2045-23222018-08-018111110.1038/s41598-018-29899-1Transcriptional insights into the pyramided resistance to rice bacterial blightLifen Gao0Zhiwei Fang1Junfei Zhou2Lun Li3Long Lu4Lili Li5Tiantian Li6Lihong Chen7Weixiong Zhang8Wenxue Zhai9Hai Peng10Institute for Systems Biology, Jianghan UniversityInstitute for Systems Biology, Jianghan UniversityInstitute for Systems Biology, Jianghan UniversityInstitute for Systems Biology, Jianghan UniversityInstitute for Systems Biology, Jianghan UniversityInstitute for Systems Biology, Jianghan UniversityInstitute for Systems Biology, Jianghan UniversityInstitute for Systems Biology, Jianghan UniversityInstitute for Systems Biology, Jianghan UniversityInstitute of Genetics and Developmental Biology, Chinese Academy of SciencesInstitute for Systems Biology, Jianghan UniversityAbstract The pyramiding of resistance (R) genes provides broad-spectrum and durable resistance to plant diseases. However, the genetic basis for bacterial blight (BB) resistance remains unclear. The BB R gene pyramided line IRBB54, which expresses xa5 and Xa21, possessed a higher level of resistance than both single R gene lines. Large-scale genotyping of genetic markers in this study revealed similar genetic backgrounds among the near-isogenic lines (NILs), suggesting that resistance in the resistant NILs was mainly conferred by the individual R genes or the interaction between them. Transcriptome analysis demonstrated that more than 50% of the differentially expressed genes (DEGs), and more than 70% of the differentially expressed functions, were shared between IRBB54 and IRBB5 or IRBB21. Most of the DEGs in the resistant NILs were downregulated and are predicted to function in cellular and biological process. The DEGs common among the resistant NILs mainly showed non-additive expression patterns and enrichment in stress-related pathways. The differential expression of agronomic trait-controlled genes in the resistant NILs, especially in IRBB54, indicated the existence of potential side-effects resulting from gene pyramiding. Our findings contribute to the understanding of R gene pyramiding, as well as its effects on targeted and non-targeted trait(s).https://doi.org/10.1038/s41598-018-29899-1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lifen Gao Zhiwei Fang Junfei Zhou Lun Li Long Lu Lili Li Tiantian Li Lihong Chen Weixiong Zhang Wenxue Zhai Hai Peng |
spellingShingle |
Lifen Gao Zhiwei Fang Junfei Zhou Lun Li Long Lu Lili Li Tiantian Li Lihong Chen Weixiong Zhang Wenxue Zhai Hai Peng Transcriptional insights into the pyramided resistance to rice bacterial blight Scientific Reports |
author_facet |
Lifen Gao Zhiwei Fang Junfei Zhou Lun Li Long Lu Lili Li Tiantian Li Lihong Chen Weixiong Zhang Wenxue Zhai Hai Peng |
author_sort |
Lifen Gao |
title |
Transcriptional insights into the pyramided resistance to rice bacterial blight |
title_short |
Transcriptional insights into the pyramided resistance to rice bacterial blight |
title_full |
Transcriptional insights into the pyramided resistance to rice bacterial blight |
title_fullStr |
Transcriptional insights into the pyramided resistance to rice bacterial blight |
title_full_unstemmed |
Transcriptional insights into the pyramided resistance to rice bacterial blight |
title_sort |
transcriptional insights into the pyramided resistance to rice bacterial blight |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2018-08-01 |
description |
Abstract The pyramiding of resistance (R) genes provides broad-spectrum and durable resistance to plant diseases. However, the genetic basis for bacterial blight (BB) resistance remains unclear. The BB R gene pyramided line IRBB54, which expresses xa5 and Xa21, possessed a higher level of resistance than both single R gene lines. Large-scale genotyping of genetic markers in this study revealed similar genetic backgrounds among the near-isogenic lines (NILs), suggesting that resistance in the resistant NILs was mainly conferred by the individual R genes or the interaction between them. Transcriptome analysis demonstrated that more than 50% of the differentially expressed genes (DEGs), and more than 70% of the differentially expressed functions, were shared between IRBB54 and IRBB5 or IRBB21. Most of the DEGs in the resistant NILs were downregulated and are predicted to function in cellular and biological process. The DEGs common among the resistant NILs mainly showed non-additive expression patterns and enrichment in stress-related pathways. The differential expression of agronomic trait-controlled genes in the resistant NILs, especially in IRBB54, indicated the existence of potential side-effects resulting from gene pyramiding. Our findings contribute to the understanding of R gene pyramiding, as well as its effects on targeted and non-targeted trait(s). |
url |
https://doi.org/10.1038/s41598-018-29899-1 |
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