Hierarchical pulmonary target nanoparticles via inhaled administration for anticancer drug delivery
Inhalation administration, compared with intravenous administration, significantly enhances chemotherapeutic drug exposure to the lung tissue and may increase the therapeutic effect for pulmonary anticancer. However, further identification of cancer cells after lung deposition of inhaled drugs is ne...
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doaj-3bef994722524545a5e52c2f88abb3202020-11-25T03:29:07ZengTaylor & Francis GroupDrug Delivery1071-75441521-04642017-01-012411191120310.1080/10717544.2017.13653951365395Hierarchical pulmonary target nanoparticles via inhaled administration for anticancer drug deliveryRui Chen0Liu Xu1Qin Fan2Man Li3Jingjing Wang4Li Wu5Weidong Li6Jinao Duan7Zhipeng Chen8Nanjing University of Chinese MedicineNanjing University of Chinese MedicineNanjing University of Chinese MedicineNanjing University of Chinese MedicineNanjing University of Chinese MedicineNanjing University of Chinese MedicineNanjing University of Chinese MedicineNanjing University of Chinese MedicineNanjing University of Chinese MedicineInhalation administration, compared with intravenous administration, significantly enhances chemotherapeutic drug exposure to the lung tissue and may increase the therapeutic effect for pulmonary anticancer. However, further identification of cancer cells after lung deposition of inhaled drugs is necessary to avoid side effects on normal lung tissue and to maximize drug efficacy. Moreover, as the action site of the major drug was intracellular organelles, drug target to the specific organelle is the final key for accurate drug delivery. Here, we designed a novel multifunctional nanoparticles (MNPs) for pulmonary antitumor and the material was well-designed for hierarchical target involved lung tissue target, cancer cell target, and mitochondrial target. The biodistribution in vivo determined by UHPLC–MS/MS method was employed to verify the drug concentration overwhelmingly increasing in lung tissue through inhaled administration compared with intravenous administration. Cellular uptake assay using A549 cells proved the efficient receptor-mediated cell endocytosis. Confocal laser scanning microscopy observation showed the location of MNPs in cells was mitochondria. All results confirmed the intelligent material can progressively play hierarchical target functions, which could induce more cell apoptosis related to mitochondrial damage. It provides a smart and efficient nanocarrier platform for hierarchical targeting of pulmonary anticancer drug. So far, this kind of material for pulmonary mitochondrial-target has not been seen in other reports.http://dx.doi.org/10.1080/10717544.2017.1365395inhaled administrationhierarchical targetmitochondriadrug deliverypulmonary anticancer drug |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rui Chen Liu Xu Qin Fan Man Li Jingjing Wang Li Wu Weidong Li Jinao Duan Zhipeng Chen |
spellingShingle |
Rui Chen Liu Xu Qin Fan Man Li Jingjing Wang Li Wu Weidong Li Jinao Duan Zhipeng Chen Hierarchical pulmonary target nanoparticles via inhaled administration for anticancer drug delivery Drug Delivery inhaled administration hierarchical target mitochondria drug delivery pulmonary anticancer drug |
author_facet |
Rui Chen Liu Xu Qin Fan Man Li Jingjing Wang Li Wu Weidong Li Jinao Duan Zhipeng Chen |
author_sort |
Rui Chen |
title |
Hierarchical pulmonary target nanoparticles via inhaled administration for anticancer drug delivery |
title_short |
Hierarchical pulmonary target nanoparticles via inhaled administration for anticancer drug delivery |
title_full |
Hierarchical pulmonary target nanoparticles via inhaled administration for anticancer drug delivery |
title_fullStr |
Hierarchical pulmonary target nanoparticles via inhaled administration for anticancer drug delivery |
title_full_unstemmed |
Hierarchical pulmonary target nanoparticles via inhaled administration for anticancer drug delivery |
title_sort |
hierarchical pulmonary target nanoparticles via inhaled administration for anticancer drug delivery |
publisher |
Taylor & Francis Group |
series |
Drug Delivery |
issn |
1071-7544 1521-0464 |
publishDate |
2017-01-01 |
description |
Inhalation administration, compared with intravenous administration, significantly enhances chemotherapeutic drug exposure to the lung tissue and may increase the therapeutic effect for pulmonary anticancer. However, further identification of cancer cells after lung deposition of inhaled drugs is necessary to avoid side effects on normal lung tissue and to maximize drug efficacy. Moreover, as the action site of the major drug was intracellular organelles, drug target to the specific organelle is the final key for accurate drug delivery. Here, we designed a novel multifunctional nanoparticles (MNPs) for pulmonary antitumor and the material was well-designed for hierarchical target involved lung tissue target, cancer cell target, and mitochondrial target. The biodistribution in vivo determined by UHPLC–MS/MS method was employed to verify the drug concentration overwhelmingly increasing in lung tissue through inhaled administration compared with intravenous administration. Cellular uptake assay using A549 cells proved the efficient receptor-mediated cell endocytosis. Confocal laser scanning microscopy observation showed the location of MNPs in cells was mitochondria. All results confirmed the intelligent material can progressively play hierarchical target functions, which could induce more cell apoptosis related to mitochondrial damage. It provides a smart and efficient nanocarrier platform for hierarchical targeting of pulmonary anticancer drug. So far, this kind of material for pulmonary mitochondrial-target has not been seen in other reports. |
topic |
inhaled administration hierarchical target mitochondria drug delivery pulmonary anticancer drug |
url |
http://dx.doi.org/10.1080/10717544.2017.1365395 |
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