Distribution of Class D Carbapenemase and Extended-Spectrum β-Lactamase Genes among Acinetobacter Baumannii Isolated from Burn Wound and Ventilator Associated Pneumonia Infections

Introduction: Resistance to Acinetobacter baumannii is dramatically on the rise in Iran. Therefore, it is important to study resistance pattern among Acinetobacter isolates which is a common cause of nosocomial infections. Aim: To investigate antibiotic resistance patterns and the role of resist...

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Main Authors: Maryam Mohammadi, Setareh Soroush, Somayeh Delfani, Iraj Pakzad, Abolfazl Abbaszadeh, Mahmoud Bahmani, Lidija Bogdanovic, Morovat Taherikalani
Format: Article
Language:English
Published: JCDR Research and Publications Private Limited 2017-07-01
Series:Journal of Clinical and Diagnostic Research
Subjects:
Online Access:https://jcdr.net/articles/PDF/10218/25534_CE(RA1)_F(T)_PF1(Sh_PrG)_PFA(P_PrG).pdf
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spelling doaj-3bf437b1c19c42058b0009788b37323f2020-11-25T02:26:53ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2017-07-01117DC19DC2310.7860/JCDR/2017/25534.10218Distribution of Class D Carbapenemase and Extended-Spectrum β-Lactamase Genes among Acinetobacter Baumannii Isolated from Burn Wound and Ventilator Associated Pneumonia InfectionsMaryam Mohammadi0Setareh Soroush1Somayeh Delfani2Iraj Pakzad3Abolfazl Abbaszadeh4Mahmoud Bahmani5Lidija Bogdanovic6Morovat Taherikalani7Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.Razi Herbal Medicines Research Center, Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.Razi Herbal Medicines Research Center, Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.Department of Surgery, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam Iran.Department of Public Health, University of Naples Federico II, Naples, Italy.Razi Herbal Medicines Research Center, Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.Introduction: Resistance to Acinetobacter baumannii is dramatically on the rise in Iran. Therefore, it is important to study resistance pattern among Acinetobacter isolates which is a common cause of nosocomial infections. Aim: To investigate antibiotic resistance patterns and the role of resistant genes and biofilm formation in the induction of resistance among Acinetobacter baumannii isolated from burn wound and ventilator associated pneumonia infections. Materials and Methods: Total 103 isolates such as 33 burn samples from Rasool Akram Hospital and 70 isolates from ventilated patients in Shahid Motahhari Hospital were identified with A. baumannii using biochemical method, and then identified to species level with PCR of gyrB and blaOXA-51 gene. Antibiotic sensitivity pattern for b-lactam and carbapenem antibiotics was assessed using Agar disc diffusion test and E-test. The presence of different carbapenemase and metalo-b-lactamase (blaOXA-51-like, gyrB, blaOXA-23-like, blaOXA-24-like, blaOXA-58, blaVEB, blaPER, blaGIM, blaSIM, blaIMP, blaVIM), extended-spectrum β-lactamases (blaTEM, blaSHV) and two insertion sequences genes (ISaba1, IS1113) was assessed. Biofilm formation of all isolates was then assessed. Chi-square analysis or Fisher’s-exact tests were used for statistical analysis. A p-value <0.05 was considered statistically significant. Results: Colistin was the most effective antimicrobial agents, although 10.7% (11/103) of the isolates were resistant. The high rate of resistance to meropenem (93.2%) and imipenem (90.3%) was determined. Also, with exception of ampicillin-sulbactam, surprisingly the resistant rate was 28.2%, the resistance to b-lactam antibiotic was dramatically increased. Co-existence of two and three blaOXA genes was also determined. The blaOXA58 was detected in only one isolate. The blaTEM and blaOXA23 was the most prevalent Extended-Spectrum β-Lactamases (ESBL) gene. All isolates were biofilm producers. Conclusion: Antibiotic resistance is increasing among A. baumannii isolates which is due to excessive use of antibiotics and also acquired resistant genes and biofilm production. Resistance to nearly all antimicrobial agents especially colistin as end choice for treatment of multiple drug resistance A. baumannii is a big concern. https://jcdr.net/articles/PDF/10218/25534_CE(RA1)_F(T)_PF1(Sh_PrG)_PFA(P_PrG).pdfβ-lactamsinsertion elementsnosocomial infections
collection DOAJ
language English
format Article
sources DOAJ
author Maryam Mohammadi
Setareh Soroush
Somayeh Delfani
Iraj Pakzad
Abolfazl Abbaszadeh
Mahmoud Bahmani
Lidija Bogdanovic
Morovat Taherikalani
spellingShingle Maryam Mohammadi
Setareh Soroush
Somayeh Delfani
Iraj Pakzad
Abolfazl Abbaszadeh
Mahmoud Bahmani
Lidija Bogdanovic
Morovat Taherikalani
Distribution of Class D Carbapenemase and Extended-Spectrum β-Lactamase Genes among Acinetobacter Baumannii Isolated from Burn Wound and Ventilator Associated Pneumonia Infections
Journal of Clinical and Diagnostic Research
β-lactams
insertion elements
nosocomial infections
author_facet Maryam Mohammadi
Setareh Soroush
Somayeh Delfani
Iraj Pakzad
Abolfazl Abbaszadeh
Mahmoud Bahmani
Lidija Bogdanovic
Morovat Taherikalani
author_sort Maryam Mohammadi
title Distribution of Class D Carbapenemase and Extended-Spectrum β-Lactamase Genes among Acinetobacter Baumannii Isolated from Burn Wound and Ventilator Associated Pneumonia Infections
title_short Distribution of Class D Carbapenemase and Extended-Spectrum β-Lactamase Genes among Acinetobacter Baumannii Isolated from Burn Wound and Ventilator Associated Pneumonia Infections
title_full Distribution of Class D Carbapenemase and Extended-Spectrum β-Lactamase Genes among Acinetobacter Baumannii Isolated from Burn Wound and Ventilator Associated Pneumonia Infections
title_fullStr Distribution of Class D Carbapenemase and Extended-Spectrum β-Lactamase Genes among Acinetobacter Baumannii Isolated from Burn Wound and Ventilator Associated Pneumonia Infections
title_full_unstemmed Distribution of Class D Carbapenemase and Extended-Spectrum β-Lactamase Genes among Acinetobacter Baumannii Isolated from Burn Wound and Ventilator Associated Pneumonia Infections
title_sort distribution of class d carbapenemase and extended-spectrum β-lactamase genes among acinetobacter baumannii isolated from burn wound and ventilator associated pneumonia infections
publisher JCDR Research and Publications Private Limited
series Journal of Clinical and Diagnostic Research
issn 2249-782X
0973-709X
publishDate 2017-07-01
description Introduction: Resistance to Acinetobacter baumannii is dramatically on the rise in Iran. Therefore, it is important to study resistance pattern among Acinetobacter isolates which is a common cause of nosocomial infections. Aim: To investigate antibiotic resistance patterns and the role of resistant genes and biofilm formation in the induction of resistance among Acinetobacter baumannii isolated from burn wound and ventilator associated pneumonia infections. Materials and Methods: Total 103 isolates such as 33 burn samples from Rasool Akram Hospital and 70 isolates from ventilated patients in Shahid Motahhari Hospital were identified with A. baumannii using biochemical method, and then identified to species level with PCR of gyrB and blaOXA-51 gene. Antibiotic sensitivity pattern for b-lactam and carbapenem antibiotics was assessed using Agar disc diffusion test and E-test. The presence of different carbapenemase and metalo-b-lactamase (blaOXA-51-like, gyrB, blaOXA-23-like, blaOXA-24-like, blaOXA-58, blaVEB, blaPER, blaGIM, blaSIM, blaIMP, blaVIM), extended-spectrum β-lactamases (blaTEM, blaSHV) and two insertion sequences genes (ISaba1, IS1113) was assessed. Biofilm formation of all isolates was then assessed. Chi-square analysis or Fisher’s-exact tests were used for statistical analysis. A p-value <0.05 was considered statistically significant. Results: Colistin was the most effective antimicrobial agents, although 10.7% (11/103) of the isolates were resistant. The high rate of resistance to meropenem (93.2%) and imipenem (90.3%) was determined. Also, with exception of ampicillin-sulbactam, surprisingly the resistant rate was 28.2%, the resistance to b-lactam antibiotic was dramatically increased. Co-existence of two and three blaOXA genes was also determined. The blaOXA58 was detected in only one isolate. The blaTEM and blaOXA23 was the most prevalent Extended-Spectrum β-Lactamases (ESBL) gene. All isolates were biofilm producers. Conclusion: Antibiotic resistance is increasing among A. baumannii isolates which is due to excessive use of antibiotics and also acquired resistant genes and biofilm production. Resistance to nearly all antimicrobial agents especially colistin as end choice for treatment of multiple drug resistance A. baumannii is a big concern.
topic β-lactams
insertion elements
nosocomial infections
url https://jcdr.net/articles/PDF/10218/25534_CE(RA1)_F(T)_PF1(Sh_PrG)_PFA(P_PrG).pdf
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