Anti-Tumor Drug-Loaded Oxygen Nanobubbles for the Degradation of HIF-1α and the Upregulation of Reactive Oxygen Species in Tumor Cells

Hypoxia is a key concern during the treatment of tumors, and hypoxia-inducible factor 1 alpha (HIF-1α) has been associated with increased tumor resistance to therapeutic modalities. In this study, doxorubicin-loaded oxygen nanobubbles (Dox/ONBs) were synthesized, and the effectiveness of dr...

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Main Authors:	Muhammad Saad Khan, Jangsun Hwang, Kyungwoo Lee, Yonghyun Choi, Youngmin Seo, Hojeong Jeon, Jong Wook Hong, Jonghoon Choi
Format:	Article
Language:	English
Published:	MDPI AG 2019-09-01
Series:	Cancers
Subjects:	doxorubicin drug delivery oxygen nanobubbles hypoxia hypoxia-inducible factor 1 alpha
Online Access:	https://www.mdpi.com/2072-6694/11/10/1464

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spelling	doaj-3bf545d670ca400d92d3f3411ab7b83b2020-11-25T01:23:42ZengMDPI AGCancers2072-66942019-09-011110146410.3390/cancers11101464cancers11101464Anti-Tumor Drug-Loaded Oxygen Nanobubbles for the Degradation of HIF-1α and the Upregulation of Reactive Oxygen Species in Tumor CellsMuhammad Saad Khan0Jangsun Hwang1Kyungwoo Lee2Yonghyun Choi3Youngmin Seo4Hojeong Jeon5Jong Wook Hong6Jonghoon Choi7School of Integrative Engineering, Chung-Ang University, Seoul 06974, KoreaSchool of Integrative Engineering, Chung-Ang University, Seoul 06974, KoreaSchool of Integrative Engineering, Chung-Ang University, Seoul 06974, KoreaSchool of Integrative Engineering, Chung-Ang University, Seoul 06974, KoreaCenter for Biomaterials, Korea Institute of Science and Technology, Seoul 02792, KoreaCenter for Biomaterials, Korea Institute of Science and Technology, Seoul 02792, KoreaDepartment of Bionano Technology, Hanyang University, Seoul 426-791, KoreaSchool of Integrative Engineering, Chung-Ang University, Seoul 06974, KoreaHypoxia is a key concern during the treatment of tumors, and hypoxia-inducible factor 1 alpha (HIF-1α) has been associated with increased tumor resistance to therapeutic modalities. In this study, doxorubicin-loaded oxygen nanobubbles (Dox/ONBs) were synthesized, and the effectiveness of drug delivery to MDA-MB-231 breast cancer and HeLa cells was evaluated. Dox/ONBs were characterized using optical and fluorescence microscopy, and size measurements were performed through nanoparticle tracking analysis (NTA). The working mechanism of Dox was evaluated using reactive oxygen species (ROS) assays, and cellular penetration was assessed with confocal microscopy. Hypoxic conditions were established to assess the effect of Dox/ONBs under hypoxic conditions compared with normoxic conditions. Our results indicate that Dox/ONBs are effective for drug delivery, enhancing oxygen levels, and ROS generation in tumor-derived cell lines.https://www.mdpi.com/2072-6694/11/10/1464doxorubicindrug deliveryoxygen nanobubbleshypoxiahypoxia-inducible factor 1 alpha
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Muhammad Saad Khan Jangsun Hwang Kyungwoo Lee Yonghyun Choi Youngmin Seo Hojeong Jeon Jong Wook Hong Jonghoon Choi
spellingShingle	Muhammad Saad Khan Jangsun Hwang Kyungwoo Lee Yonghyun Choi Youngmin Seo Hojeong Jeon Jong Wook Hong Jonghoon Choi Anti-Tumor Drug-Loaded Oxygen Nanobubbles for the Degradation of HIF-1α and the Upregulation of Reactive Oxygen Species in Tumor Cells Cancers doxorubicin drug delivery oxygen nanobubbles hypoxia hypoxia-inducible factor 1 alpha
author_facet	Muhammad Saad Khan Jangsun Hwang Kyungwoo Lee Yonghyun Choi Youngmin Seo Hojeong Jeon Jong Wook Hong Jonghoon Choi
author_sort	Muhammad Saad Khan
title	Anti-Tumor Drug-Loaded Oxygen Nanobubbles for the Degradation of HIF-1α and the Upregulation of Reactive Oxygen Species in Tumor Cells
title_short	Anti-Tumor Drug-Loaded Oxygen Nanobubbles for the Degradation of HIF-1α and the Upregulation of Reactive Oxygen Species in Tumor Cells
title_full	Anti-Tumor Drug-Loaded Oxygen Nanobubbles for the Degradation of HIF-1α and the Upregulation of Reactive Oxygen Species in Tumor Cells
title_fullStr	Anti-Tumor Drug-Loaded Oxygen Nanobubbles for the Degradation of HIF-1α and the Upregulation of Reactive Oxygen Species in Tumor Cells
title_full_unstemmed	Anti-Tumor Drug-Loaded Oxygen Nanobubbles for the Degradation of HIF-1α and the Upregulation of Reactive Oxygen Species in Tumor Cells
title_sort	anti-tumor drug-loaded oxygen nanobubbles for the degradation of hif-1α and the upregulation of reactive oxygen species in tumor cells
publisher	MDPI AG
series	Cancers
issn	2072-6694
publishDate	2019-09-01
description	Hypoxia is a key concern during the treatment of tumors, and hypoxia-inducible factor 1 alpha (HIF-1α) has been associated with increased tumor resistance to therapeutic modalities. In this study, doxorubicin-loaded oxygen nanobubbles (Dox/ONBs) were synthesized, and the effectiveness of drug delivery to MDA-MB-231 breast cancer and HeLa cells was evaluated. Dox/ONBs were characterized using optical and fluorescence microscopy, and size measurements were performed through nanoparticle tracking analysis (NTA). The working mechanism of Dox was evaluated using reactive oxygen species (ROS) assays, and cellular penetration was assessed with confocal microscopy. Hypoxic conditions were established to assess the effect of Dox/ONBs under hypoxic conditions compared with normoxic conditions. Our results indicate that Dox/ONBs are effective for drug delivery, enhancing oxygen levels, and ROS generation in tumor-derived cell lines.
topic	doxorubicin drug delivery oxygen nanobubbles hypoxia hypoxia-inducible factor 1 alpha
url	https://www.mdpi.com/2072-6694/11/10/1464
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Anti-Tumor Drug-Loaded Oxygen Nanobubbles for the Degradation of HIF-1α and the Upregulation of Reactive Oxygen Species in Tumor Cells

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