Proline Biosynthesis Enzyme Genes Confer Salt Tolerance to Switchgrass (Panicum virgatum L.) in Cooperation With Polyamines Metabolism
Understanding the regulation of proline metabolism necessitates the suppression of two Δ1-pyrroline-5-carboxylate synthetase enzyme (P5CS) genes performed in switchgrass (Panicum virgatum L.). The results reveal that overexpressing PvP5CS1 and PvP5CS2 increased salt tolerance. Additionally, transcri...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2020-02-01
|
Series: | Frontiers in Plant Science |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.00046/full |
id |
doaj-3c04276f6b9f427aa7e74f1673832667 |
---|---|
record_format |
Article |
spelling |
doaj-3c04276f6b9f427aa7e74f16738326672020-11-25T02:26:55ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-02-011110.3389/fpls.2020.00046511538Proline Biosynthesis Enzyme Genes Confer Salt Tolerance to Switchgrass (Panicum virgatum L.) in Cooperation With Polyamines MetabolismCong Guan0Xin Cui1Hua-yue Liu2Xue Li3Meng-qi Li4 Yun-wei Zhang5 Yun-wei Zhang6 Yun-wei Zhang7 Yun-wei Zhang8College of Grassland Science and Technology, China Agricultural University, Beijing, ChinaCollege of Grassland Science and Technology, China Agricultural University, Beijing, ChinaCollege of Grassland Science and Technology, China Agricultural University, Beijing, ChinaCollege of Grassland Science and Technology, China Agricultural University, Beijing, ChinaCollege of Grassland Science and Technology, China Agricultural University, Beijing, ChinaCollege of Grassland Science and Technology, China Agricultural University, Beijing, ChinaBeijing Key Laboratory for Grassland Science, China Agricultural University, Beijing, ChinaNational Energy R&D Center for Biomass (NECB), Beijing, ChinaBeijing Sure Academy of Biosciences, Beijing, ChinaUnderstanding the regulation of proline metabolism necessitates the suppression of two Δ1-pyrroline-5-carboxylate synthetase enzyme (P5CS) genes performed in switchgrass (Panicum virgatum L.). The results reveal that overexpressing PvP5CS1 and PvP5CS2 increased salt tolerance. Additionally, transcript levels of spermidine (Spd) and spermine (Spm) synthesis and metabolism related genes were upregulated in PvP5CS OE-transgenic plants and downregulated in the PvP5CS RNAi transformants. According to salt stress assay and the measurement of transcript levels of Polyamines (PAs) metabolism-related genes, P5CS enzyme may not only be the key regulator of proline biosynthesis in switchgrass, but it may also indirectly affect the entire subset of pathway for ornithine to proline or to putrescine (Put). Furthermore, application of proline prompted expression levels of Spd and Spm synthesis and metabolism-related genes in both PvP5CS-RNAi and WT plants, but transcript levels were even lower in PvP5CS-RNAi compared to WT plants under salt stress condition. These results suggested that exogenous proline could accelerate polyamines metabolisms under salt stress. Nevertheless, the enzymes involved in this process and the potential functions remain poorly understood. Thus, the aim of this study is to reveal how proline functions with PAs metabolism under salt stress in switchgrass.https://www.frontiersin.org/article/10.3389/fpls.2020.00046/fullswitchgrassPvP5CS1 and PvP5CS2prolinepolyaminessalt stress |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cong Guan Xin Cui Hua-yue Liu Xue Li Meng-qi Li Yun-wei Zhang Yun-wei Zhang Yun-wei Zhang Yun-wei Zhang |
spellingShingle |
Cong Guan Xin Cui Hua-yue Liu Xue Li Meng-qi Li Yun-wei Zhang Yun-wei Zhang Yun-wei Zhang Yun-wei Zhang Proline Biosynthesis Enzyme Genes Confer Salt Tolerance to Switchgrass (Panicum virgatum L.) in Cooperation With Polyamines Metabolism Frontiers in Plant Science switchgrass PvP5CS1 and PvP5CS2 proline polyamines salt stress |
author_facet |
Cong Guan Xin Cui Hua-yue Liu Xue Li Meng-qi Li Yun-wei Zhang Yun-wei Zhang Yun-wei Zhang Yun-wei Zhang |
author_sort |
Cong Guan |
title |
Proline Biosynthesis Enzyme Genes Confer Salt Tolerance to Switchgrass (Panicum virgatum L.) in Cooperation With Polyamines Metabolism |
title_short |
Proline Biosynthesis Enzyme Genes Confer Salt Tolerance to Switchgrass (Panicum virgatum L.) in Cooperation With Polyamines Metabolism |
title_full |
Proline Biosynthesis Enzyme Genes Confer Salt Tolerance to Switchgrass (Panicum virgatum L.) in Cooperation With Polyamines Metabolism |
title_fullStr |
Proline Biosynthesis Enzyme Genes Confer Salt Tolerance to Switchgrass (Panicum virgatum L.) in Cooperation With Polyamines Metabolism |
title_full_unstemmed |
Proline Biosynthesis Enzyme Genes Confer Salt Tolerance to Switchgrass (Panicum virgatum L.) in Cooperation With Polyamines Metabolism |
title_sort |
proline biosynthesis enzyme genes confer salt tolerance to switchgrass (panicum virgatum l.) in cooperation with polyamines metabolism |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2020-02-01 |
description |
Understanding the regulation of proline metabolism necessitates the suppression of two Δ1-pyrroline-5-carboxylate synthetase enzyme (P5CS) genes performed in switchgrass (Panicum virgatum L.). The results reveal that overexpressing PvP5CS1 and PvP5CS2 increased salt tolerance. Additionally, transcript levels of spermidine (Spd) and spermine (Spm) synthesis and metabolism related genes were upregulated in PvP5CS OE-transgenic plants and downregulated in the PvP5CS RNAi transformants. According to salt stress assay and the measurement of transcript levels of Polyamines (PAs) metabolism-related genes, P5CS enzyme may not only be the key regulator of proline biosynthesis in switchgrass, but it may also indirectly affect the entire subset of pathway for ornithine to proline or to putrescine (Put). Furthermore, application of proline prompted expression levels of Spd and Spm synthesis and metabolism-related genes in both PvP5CS-RNAi and WT plants, but transcript levels were even lower in PvP5CS-RNAi compared to WT plants under salt stress condition. These results suggested that exogenous proline could accelerate polyamines metabolisms under salt stress. Nevertheless, the enzymes involved in this process and the potential functions remain poorly understood. Thus, the aim of this study is to reveal how proline functions with PAs metabolism under salt stress in switchgrass. |
topic |
switchgrass PvP5CS1 and PvP5CS2 proline polyamines salt stress |
url |
https://www.frontiersin.org/article/10.3389/fpls.2020.00046/full |
work_keys_str_mv |
AT congguan prolinebiosynthesisenzymegenesconfersalttolerancetoswitchgrasspanicumvirgatumlincooperationwithpolyaminesmetabolism AT xincui prolinebiosynthesisenzymegenesconfersalttolerancetoswitchgrasspanicumvirgatumlincooperationwithpolyaminesmetabolism AT huayueliu prolinebiosynthesisenzymegenesconfersalttolerancetoswitchgrasspanicumvirgatumlincooperationwithpolyaminesmetabolism AT xueli prolinebiosynthesisenzymegenesconfersalttolerancetoswitchgrasspanicumvirgatumlincooperationwithpolyaminesmetabolism AT mengqili prolinebiosynthesisenzymegenesconfersalttolerancetoswitchgrasspanicumvirgatumlincooperationwithpolyaminesmetabolism AT yunweizhang prolinebiosynthesisenzymegenesconfersalttolerancetoswitchgrasspanicumvirgatumlincooperationwithpolyaminesmetabolism AT yunweizhang prolinebiosynthesisenzymegenesconfersalttolerancetoswitchgrasspanicumvirgatumlincooperationwithpolyaminesmetabolism AT yunweizhang prolinebiosynthesisenzymegenesconfersalttolerancetoswitchgrasspanicumvirgatumlincooperationwithpolyaminesmetabolism AT yunweizhang prolinebiosynthesisenzymegenesconfersalttolerancetoswitchgrasspanicumvirgatumlincooperationwithpolyaminesmetabolism |
_version_ |
1724845121738899456 |