Simulated Weightlessness Perturbs the Intestinal Metabolomic Profile of Rats

Recently, disorders of intestinal homeostasis in the space environment have been extensively demonstrated. Accumulating evidence have suggested microgravity and simulated weightlessness could induce dysbiosis of intestinal microbiota, which may contribute to the bowel symptoms during spaceflight. Ho...

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Main Authors: Mingliang Jin, Jiaojiao Wang, Hao Zhang, Hongbin Zhou, Ke Zhao
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-10-01
Series:Frontiers in Physiology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fphys.2019.01279/full
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spelling doaj-3c09832a3cfe4496ba9e90ffa089215e2020-11-25T01:51:05ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2019-10-011010.3389/fphys.2019.01279476141Simulated Weightlessness Perturbs the Intestinal Metabolomic Profile of RatsMingliang Jin0Mingliang Jin1Jiaojiao Wang2Hao Zhang3Hongbin Zhou4Ke Zhao5College of Animal Sciences, Zhejiang University, Hangzhou, ChinaSchool of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaSchool of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaSchool of Life Sciences, Northwestern Polytechnical University, Xi’an, ChinaDalian Chengsan Animal Husbandry Co., Ltd., Dalian, ChinaCollege of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an, ChinaRecently, disorders of intestinal homeostasis in the space environment have been extensively demonstrated. Accumulating evidence have suggested microgravity and simulated weightlessness could induce dysbiosis of intestinal microbiota, which may contribute to the bowel symptoms during spaceflight. However, the specific responses of intestinal metabolome under simulated weightlessness and its relationship with the intestinal microbiome and immune characteristics remain largely unknown. In the current study, 20 adult Sprague-Dawley (SD) rats were randomly divided into the control group and the simulated weightlessness group using a hindlimb unloading model. The metabolomic profiling of cecal contents from eight rats of each group was investigated by gas chromatography-time of flight/mass spectrometry. The significantly different metabolites, biomarkers, and related pathways were identified. Multivariate analysis, such as principal component analysis and orthogonal projections to latent structures-discriminant analysis, demonstrated an obvious separation between the control group and the simulated weightlessness group. Significantly different metabolites, such as xylose, sinapinic acid, indolelactate, and digalacturonic acid, were identified, which participate in mainly pyrimidine metabolism, pentose and glucuronate interconversions, and valine, leucine and isoleucine metabolism. Cytidine-5′-monophosphate, 4-hydroxypyridine, and phloretic acid were determined as pivotal biomarkers under simulated weightlessness. Moreover, the significantly different metabolites were remarkably correlated with dysbiosis of the intestinal microbiota and disturbance of immunological characteristics induced by simulated weightlessness. These metabolic features provide crucial candidates for therapeutic targets for metabolic disorders under weightlessness.https://www.frontiersin.org/article/10.3389/fphys.2019.01279/fullsimulated weightlessnessintestinemetabolomicsmicrobiotaimmunity
collection DOAJ
language English
format Article
sources DOAJ
author Mingliang Jin
Mingliang Jin
Jiaojiao Wang
Hao Zhang
Hongbin Zhou
Ke Zhao
spellingShingle Mingliang Jin
Mingliang Jin
Jiaojiao Wang
Hao Zhang
Hongbin Zhou
Ke Zhao
Simulated Weightlessness Perturbs the Intestinal Metabolomic Profile of Rats
Frontiers in Physiology
simulated weightlessness
intestine
metabolomics
microbiota
immunity
author_facet Mingliang Jin
Mingliang Jin
Jiaojiao Wang
Hao Zhang
Hongbin Zhou
Ke Zhao
author_sort Mingliang Jin
title Simulated Weightlessness Perturbs the Intestinal Metabolomic Profile of Rats
title_short Simulated Weightlessness Perturbs the Intestinal Metabolomic Profile of Rats
title_full Simulated Weightlessness Perturbs the Intestinal Metabolomic Profile of Rats
title_fullStr Simulated Weightlessness Perturbs the Intestinal Metabolomic Profile of Rats
title_full_unstemmed Simulated Weightlessness Perturbs the Intestinal Metabolomic Profile of Rats
title_sort simulated weightlessness perturbs the intestinal metabolomic profile of rats
publisher Frontiers Media S.A.
series Frontiers in Physiology
issn 1664-042X
publishDate 2019-10-01
description Recently, disorders of intestinal homeostasis in the space environment have been extensively demonstrated. Accumulating evidence have suggested microgravity and simulated weightlessness could induce dysbiosis of intestinal microbiota, which may contribute to the bowel symptoms during spaceflight. However, the specific responses of intestinal metabolome under simulated weightlessness and its relationship with the intestinal microbiome and immune characteristics remain largely unknown. In the current study, 20 adult Sprague-Dawley (SD) rats were randomly divided into the control group and the simulated weightlessness group using a hindlimb unloading model. The metabolomic profiling of cecal contents from eight rats of each group was investigated by gas chromatography-time of flight/mass spectrometry. The significantly different metabolites, biomarkers, and related pathways were identified. Multivariate analysis, such as principal component analysis and orthogonal projections to latent structures-discriminant analysis, demonstrated an obvious separation between the control group and the simulated weightlessness group. Significantly different metabolites, such as xylose, sinapinic acid, indolelactate, and digalacturonic acid, were identified, which participate in mainly pyrimidine metabolism, pentose and glucuronate interconversions, and valine, leucine and isoleucine metabolism. Cytidine-5′-monophosphate, 4-hydroxypyridine, and phloretic acid were determined as pivotal biomarkers under simulated weightlessness. Moreover, the significantly different metabolites were remarkably correlated with dysbiosis of the intestinal microbiota and disturbance of immunological characteristics induced by simulated weightlessness. These metabolic features provide crucial candidates for therapeutic targets for metabolic disorders under weightlessness.
topic simulated weightlessness
intestine
metabolomics
microbiota
immunity
url https://www.frontiersin.org/article/10.3389/fphys.2019.01279/full
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