How Long Does a Neutrophil Live?—The Effect of 24 h Whole Blood Storage on Neutrophil Functions in Pigs
Neutrophils are important effector cells of the innate immune system, traditionally regarded to have a short life span. The goal of this study was to evaluate the effect of the whole blood storage on neutrophil functions, e.g., viability, antimicrobial effect, neutrophil extracellular trap (NET) for...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-08-01
|
Series: | Biomedicines |
Subjects: | |
Online Access: | https://www.mdpi.com/2227-9059/8/8/278 |
id |
doaj-3c192447845a4626a8247577258b00b2 |
---|---|
record_format |
Article |
spelling |
doaj-3c192447845a4626a8247577258b00b22020-11-25T03:26:36ZengMDPI AGBiomedicines2227-90592020-08-01827827810.3390/biomedicines8080278How Long Does a Neutrophil Live?—The Effect of 24 h Whole Blood Storage on Neutrophil Functions in PigsMarta C. Bonilla0Leonie Fingerhut1Adriana Alfonso-Castro2AhmedElmontaser Mergani3Cornelia Schwennen4Maren von Köckritz-Blickwede5Nicole de Buhr6Institute for Physiological Chemistry, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, GermanyInstitute for Physiological Chemistry, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, GermanyInstitute for Physiological Chemistry, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, GermanyInstitute for Physiological Chemistry, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, GermanyClinic for Swine, Small Ruminants and Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, 30173 Hannover, GermanyInstitute for Physiological Chemistry, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, GermanyInstitute for Physiological Chemistry, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, GermanyNeutrophils are important effector cells of the innate immune system, traditionally regarded to have a short life span. The goal of this study was to evaluate the effect of the whole blood storage on neutrophil functions, e.g., viability, antimicrobial effect, neutrophil extracellular trap (NET) formation and phagocytosis. Therefore, fresh porcine whole blood was compared to whole blood stored for 24 h in the dark at room temperature. Different cell parameters in whole blood and in isolated neutrophils were analyzed. The following parameters were analyzed: cell count, band and segmented neutrophil count, viability, cholesterol content, release of free DNA as a marker for cell death, phagocytic activity in whole blood and in isolated neutrophils, the transmigration rate of neutrophils to IL8 stimulus, the production of reactive oxygen species (ROS), and the formation of NETs. It was observed that the number of isolated neutrophils decreased over time, indicating cell death occurs during 24 h of blood storage. However, the surviving neutrophils isolated from stored blood reacted comparably or even showed enhanced antimicrobial activity in the case of phagocytosis of <i>Streptococcus</i><i> </i>(<i>S.</i>)<i> suis</i>, ROS production, and transmigration. The slightly altered cholesterol level of the harvested neutrophils in stored blood when compared to fresh blood partially explains some of the detected differences.https://www.mdpi.com/2227-9059/8/8/278neutrophilslife spanporcine bloodantimicrobial activityreactive oxygen species (ROS)neutrophil extracellular trap (NET) formation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marta C. Bonilla Leonie Fingerhut Adriana Alfonso-Castro AhmedElmontaser Mergani Cornelia Schwennen Maren von Köckritz-Blickwede Nicole de Buhr |
spellingShingle |
Marta C. Bonilla Leonie Fingerhut Adriana Alfonso-Castro AhmedElmontaser Mergani Cornelia Schwennen Maren von Köckritz-Blickwede Nicole de Buhr How Long Does a Neutrophil Live?—The Effect of 24 h Whole Blood Storage on Neutrophil Functions in Pigs Biomedicines neutrophils life span porcine blood antimicrobial activity reactive oxygen species (ROS) neutrophil extracellular trap (NET) formation |
author_facet |
Marta C. Bonilla Leonie Fingerhut Adriana Alfonso-Castro AhmedElmontaser Mergani Cornelia Schwennen Maren von Köckritz-Blickwede Nicole de Buhr |
author_sort |
Marta C. Bonilla |
title |
How Long Does a Neutrophil Live?—The Effect of 24 h Whole Blood Storage on Neutrophil Functions in Pigs |
title_short |
How Long Does a Neutrophil Live?—The Effect of 24 h Whole Blood Storage on Neutrophil Functions in Pigs |
title_full |
How Long Does a Neutrophil Live?—The Effect of 24 h Whole Blood Storage on Neutrophil Functions in Pigs |
title_fullStr |
How Long Does a Neutrophil Live?—The Effect of 24 h Whole Blood Storage on Neutrophil Functions in Pigs |
title_full_unstemmed |
How Long Does a Neutrophil Live?—The Effect of 24 h Whole Blood Storage on Neutrophil Functions in Pigs |
title_sort |
how long does a neutrophil live?—the effect of 24 h whole blood storage on neutrophil functions in pigs |
publisher |
MDPI AG |
series |
Biomedicines |
issn |
2227-9059 |
publishDate |
2020-08-01 |
description |
Neutrophils are important effector cells of the innate immune system, traditionally regarded to have a short life span. The goal of this study was to evaluate the effect of the whole blood storage on neutrophil functions, e.g., viability, antimicrobial effect, neutrophil extracellular trap (NET) formation and phagocytosis. Therefore, fresh porcine whole blood was compared to whole blood stored for 24 h in the dark at room temperature. Different cell parameters in whole blood and in isolated neutrophils were analyzed. The following parameters were analyzed: cell count, band and segmented neutrophil count, viability, cholesterol content, release of free DNA as a marker for cell death, phagocytic activity in whole blood and in isolated neutrophils, the transmigration rate of neutrophils to IL8 stimulus, the production of reactive oxygen species (ROS), and the formation of NETs. It was observed that the number of isolated neutrophils decreased over time, indicating cell death occurs during 24 h of blood storage. However, the surviving neutrophils isolated from stored blood reacted comparably or even showed enhanced antimicrobial activity in the case of phagocytosis of <i>Streptococcus</i><i> </i>(<i>S.</i>)<i> suis</i>, ROS production, and transmigration. The slightly altered cholesterol level of the harvested neutrophils in stored blood when compared to fresh blood partially explains some of the detected differences. |
topic |
neutrophils life span porcine blood antimicrobial activity reactive oxygen species (ROS) neutrophil extracellular trap (NET) formation |
url |
https://www.mdpi.com/2227-9059/8/8/278 |
work_keys_str_mv |
AT martacbonilla howlongdoesaneutrophillivetheeffectof24hwholebloodstorageonneutrophilfunctionsinpigs AT leoniefingerhut howlongdoesaneutrophillivetheeffectof24hwholebloodstorageonneutrophilfunctionsinpigs AT adrianaalfonsocastro howlongdoesaneutrophillivetheeffectof24hwholebloodstorageonneutrophilfunctionsinpigs AT ahmedelmontasermergani howlongdoesaneutrophillivetheeffectof24hwholebloodstorageonneutrophilfunctionsinpigs AT corneliaschwennen howlongdoesaneutrophillivetheeffectof24hwholebloodstorageonneutrophilfunctionsinpigs AT marenvonkockritzblickwede howlongdoesaneutrophillivetheeffectof24hwholebloodstorageonneutrophilfunctionsinpigs AT nicoledebuhr howlongdoesaneutrophillivetheeffectof24hwholebloodstorageonneutrophilfunctionsinpigs |
_version_ |
1724591853799473152 |