Effect of phagocytic load on apoptosis of cultured aged bovine retinal pigment epithelium
AIM:To evaluate the effect of phagocytic load on apoptosis of cultured aged bovine retinal pigment epithelium(RPE)cells.<p>METHODS: The third generation of cultured aged bovine RPE cells was cultured respectively with culture medium, containing 200μg/mL photoreceptor outer segment(POS)and 400μ...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2014-11-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/cn_publish/2014/11/201411005.pdf |
| Summary: | AIM:To evaluate the effect of phagocytic load on apoptosis of cultured aged bovine retinal pigment epithelium(RPE)cells.<p>METHODS: The third generation of cultured aged bovine RPE cells was cultured respectively with culture medium, containing 200μg/mL photoreceptor outer segment(POS)and 400μg/mL POS. Then the apoptosis rates of RPE cells after 24, 48 and 96h were detected with flow cytometry instrument(AnnexinⅤ/PI double staining).<p>RESULTS: In blank control group, the apoptosis rates were very low at different time. After RPE cells were cultured with culture medium containing 200μg/mL POS for 24h, the apoptosis rate including early apoptosis and late apoptosis was 1.73%. As time going, the apoptosis rate increased rapidly and reached 65.92% after 96h. After RPE cells were cultured with culture medium containing 400μg/mL POS for 24h, the apoptosis rate was 4.02%. And it reached 91.34% significantly after 96h.<p>CONCLUSION: Phagocytic loads can induce the apoptosis of RPE depending the time and dose. |
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| ISSN: | 1672-5123 1672-5123 |