Bioluminescent optogenetic (BL-OG) activation of neurons during mouse postnatal brain development

Summary: Bioluminescent optogenetics (BL-OG) allows activation of photosensory proteins, such as opsins, by either fiberoptics or by administering a luciferin. BL-OG thus confers both optogenetic and chemogenetic access within the same genetically targeted neuron. This bimodality offers a powerful a...

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Main Authors:	Emmanuel L. Crespo, Mansi Prakash, Andreas Bjorefeldt, William E. Medendorp, Nathan C. Shaner, Diane Lipscombe, Christopher I. Moore, Ute Hochgeschwender
Format:	Article
Language:	English
Published:	Elsevier 2021-09-01
Series:	STAR Protocols
Subjects:	Developmental biology Microscopy Model Organisms Molecular Biology Neuroscience Biotechnology and bioengineering
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166721003749

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spelling	doaj-3c21176793034b0dae2223b29eebc3f32021-09-19T05:00:19ZengElsevierSTAR Protocols2666-16672021-09-0123100667Bioluminescent optogenetic (BL-OG) activation of neurons during mouse postnatal brain developmentEmmanuel L. Crespo0Mansi Prakash1Andreas Bjorefeldt2William E. Medendorp3Nathan C. Shaner4Diane Lipscombe5Christopher I. Moore6Ute Hochgeschwender7Biochemistry, Cellular and Molecular Biology Graduate program, Central Michigan University, Mount Pleasant, MI 48859, USA; Corresponding authorCollege of Medicine, Central Michigan University, Mount Pleasant, MI 48859, USACollege of Medicine, Central Michigan University, Mount Pleasant, MI 48859, USANeuroscience Graduate Program, Central Michigan University, Mount Pleasant, MI 48859, USADepartment of Neuroscience, University of California San Diego, La Jolla, CA, USACarney Institute for Brain Science, Brown University, Providence, RI 02906, USACarney Institute for Brain Science, Brown University, Providence, RI 02906, USABiochemistry, Cellular and Molecular Biology Graduate program, Central Michigan University, Mount Pleasant, MI 48859, USA; College of Medicine, Central Michigan University, Mount Pleasant, MI 48859, USA; Neuroscience Graduate Program, Central Michigan University, Mount Pleasant, MI 48859, USA; Corresponding authorSummary: Bioluminescent optogenetics (BL-OG) allows activation of photosensory proteins, such as opsins, by either fiberoptics or by administering a luciferin. BL-OG thus confers both optogenetic and chemogenetic access within the same genetically targeted neuron. This bimodality offers a powerful approach for non-invasive chemogenetic manipulation of neural activity during brain development and adult behaviors with standard optogenetic spatiotemporal precision. We detail protocols for bioluminescent stimulation of neurons in postnatally developing brain and its validation through bioluminescence imaging and electrophysiological recording in mice.For complete information on the use and execution of this protocol, please refer to Medendorp et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721003749Developmental biologyMicroscopyModel OrganismsMolecular BiologyNeuroscienceBiotechnology and bioengineering
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Emmanuel L. Crespo Mansi Prakash Andreas Bjorefeldt William E. Medendorp Nathan C. Shaner Diane Lipscombe Christopher I. Moore Ute Hochgeschwender
spellingShingle	Emmanuel L. Crespo Mansi Prakash Andreas Bjorefeldt William E. Medendorp Nathan C. Shaner Diane Lipscombe Christopher I. Moore Ute Hochgeschwender Bioluminescent optogenetic (BL-OG) activation of neurons during mouse postnatal brain development STAR Protocols Developmental biology Microscopy Model Organisms Molecular Biology Neuroscience Biotechnology and bioengineering
author_facet	Emmanuel L. Crespo Mansi Prakash Andreas Bjorefeldt William E. Medendorp Nathan C. Shaner Diane Lipscombe Christopher I. Moore Ute Hochgeschwender
author_sort	Emmanuel L. Crespo
title	Bioluminescent optogenetic (BL-OG) activation of neurons during mouse postnatal brain development
title_short	Bioluminescent optogenetic (BL-OG) activation of neurons during mouse postnatal brain development
title_full	Bioluminescent optogenetic (BL-OG) activation of neurons during mouse postnatal brain development
title_fullStr	Bioluminescent optogenetic (BL-OG) activation of neurons during mouse postnatal brain development
title_full_unstemmed	Bioluminescent optogenetic (BL-OG) activation of neurons during mouse postnatal brain development
title_sort	bioluminescent optogenetic (bl-og) activation of neurons during mouse postnatal brain development
publisher	Elsevier
series	STAR Protocols
issn	2666-1667
publishDate	2021-09-01
description	Summary: Bioluminescent optogenetics (BL-OG) allows activation of photosensory proteins, such as opsins, by either fiberoptics or by administering a luciferin. BL-OG thus confers both optogenetic and chemogenetic access within the same genetically targeted neuron. This bimodality offers a powerful approach for non-invasive chemogenetic manipulation of neural activity during brain development and adult behaviors with standard optogenetic spatiotemporal precision. We detail protocols for bioluminescent stimulation of neurons in postnatally developing brain and its validation through bioluminescence imaging and electrophysiological recording in mice.For complete information on the use and execution of this protocol, please refer to Medendorp et al. (2021).
topic	Developmental biology Microscopy Model Organisms Molecular Biology Neuroscience Biotechnology and bioengineering
url	http://www.sciencedirect.com/science/article/pii/S2666166721003749
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Bioluminescent optogenetic (BL-OG) activation of neurons during mouse postnatal brain development

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