Enterobacteriaceae in gut of honey bee (Apis mellifera) and the antibiotic resistance of the isolates
Bacterial species of Enterobacteriaceae and the antimicrobial resistance of the isolates were detected in Apis mellifera L. bees gut. Gut content was cultivated on Meat peptone and McConkey agars at 30 and 37 °C, then, the isolates were identified with MALDI TOF MS Biotyper. Isolated strains were te...
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2017-11-01
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doaj-3c21b87797c04b07abf74e1d8d8c35b62020-11-25T03:59:42ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762017-11-0150269722038Enterobacteriaceae in gut of honey bee (Apis mellifera) and the antibiotic resistance of the isolatesJaroslav GasperMiroslava KačániováBacterial species of Enterobacteriaceae and the antimicrobial resistance of the isolates were detected in Apis mellifera L. bees gut. Gut content was cultivated on Meat peptone and McConkey agars at 30 and 37 °C, then, the isolates were identified with MALDI TOF MS Biotyper. Isolated strains were tested for antibiotic resistance to penicillins, cephalosporins, carbapenems, fluoroquinolones and aminoglycosides. Altogether, 12 species representing Enterobacteriaceae family were isolated. Firmicutes and Candida were represented by Bacillus megaterium and Issatchenkia orientalis . Isolated Enterobacteriaceae species were Enterobacter cloacae, Hafnia alvei, Klebsiella oxytoca, Morganella morganii, Serratia marcescens, Ser. liquefaciens, Raoultella ornithinolytica, R. planticola, R. terrigena, Pantoea ananatis, P. agglomerans, Rahnella aquatilis. Enterobacter cloacae, Hafnia alvei, Klebsiella oxytoca, Morganella morganii, Serratia marcescens, Ser. liquefaciens isolates exhibited the antimicrobial resistance more frequently than Raoultella ornithinolytica, R. planticola, R. terrigena, Pantoea ananatis, P. agglomerans, Rahnella aquatilis. Microflora of gut of bees could serve as a source of resistant microorganisms.http://spasb.ro/index.php/spasb/article/view/2314apis mellifera, intestinal microflora, maldi tof ms biotyper, antibiotic resistance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jaroslav Gasper Miroslava Kačániová |
spellingShingle |
Jaroslav Gasper Miroslava Kačániová Enterobacteriaceae in gut of honey bee (Apis mellifera) and the antibiotic resistance of the isolates Scientific Papers Animal Science and Biotechnologies apis mellifera, intestinal microflora, maldi tof ms biotyper, antibiotic resistance |
author_facet |
Jaroslav Gasper Miroslava Kačániová |
author_sort |
Jaroslav Gasper |
title |
Enterobacteriaceae in gut of honey bee (Apis mellifera) and the antibiotic resistance of the isolates |
title_short |
Enterobacteriaceae in gut of honey bee (Apis mellifera) and the antibiotic resistance of the isolates |
title_full |
Enterobacteriaceae in gut of honey bee (Apis mellifera) and the antibiotic resistance of the isolates |
title_fullStr |
Enterobacteriaceae in gut of honey bee (Apis mellifera) and the antibiotic resistance of the isolates |
title_full_unstemmed |
Enterobacteriaceae in gut of honey bee (Apis mellifera) and the antibiotic resistance of the isolates |
title_sort |
enterobacteriaceae in gut of honey bee (apis mellifera) and the antibiotic resistance of the isolates |
publisher |
Agroprint Timisoara |
series |
Scientific Papers Animal Science and Biotechnologies |
issn |
1841-9364 2344-4576 |
publishDate |
2017-11-01 |
description |
Bacterial species of Enterobacteriaceae and the antimicrobial resistance of the isolates were detected in Apis mellifera L. bees gut. Gut content was cultivated on Meat peptone and McConkey agars at 30 and 37 °C, then, the isolates were identified with MALDI TOF MS Biotyper. Isolated strains were tested for antibiotic resistance to penicillins, cephalosporins, carbapenems, fluoroquinolones and aminoglycosides. Altogether, 12 species representing Enterobacteriaceae family were isolated. Firmicutes and Candida were represented by Bacillus megaterium and Issatchenkia orientalis . Isolated Enterobacteriaceae species were Enterobacter cloacae, Hafnia alvei, Klebsiella oxytoca, Morganella morganii, Serratia marcescens, Ser. liquefaciens, Raoultella ornithinolytica, R. planticola, R. terrigena, Pantoea ananatis, P. agglomerans, Rahnella aquatilis. Enterobacter cloacae, Hafnia alvei, Klebsiella oxytoca, Morganella morganii, Serratia marcescens, Ser. liquefaciens isolates exhibited the antimicrobial resistance more frequently than Raoultella ornithinolytica, R. planticola, R. terrigena, Pantoea ananatis, P. agglomerans, Rahnella aquatilis. Microflora of gut of bees could serve as a source of resistant microorganisms. |
topic |
apis mellifera, intestinal microflora, maldi tof ms biotyper, antibiotic resistance |
url |
http://spasb.ro/index.php/spasb/article/view/2314 |
work_keys_str_mv |
AT jaroslavgasper enterobacteriaceaeingutofhoneybeeapismelliferaandtheantibioticresistanceoftheisolates AT miroslavakacaniova enterobacteriaceaeingutofhoneybeeapismelliferaandtheantibioticresistanceoftheisolates |
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