Caspofungin-Non-Susceptible Candida orthopsilosis Isolated from Onychomycosis in Iran
Background: Although Candida albicans remains the most common fungal isolate from clinical specimens, many studies have detected a shift towards non-albicans Candida species. Despite worrying clinical pictures associated with latter species, there is little information regarding its susceptibility...
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Tehran University of Medical Sciences
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doaj-3c404505298248498038334461f7e25f2021-01-02T15:42:06ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932017-02-01462Caspofungin-Non-Susceptible Candida orthopsilosis Isolated from Onychomycosis in IranRasoul MOHAMMADI0Hossein MIRHENDI1Mohammad Taghi HEDAYATI2Hamid BADALI3Dept. of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran AND Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, IranDept. of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran AND Dept. of Medical Parasitology and Mycology, School of Public Health, National Institute of Health Research, Tehran University of Medical Sciences, Tehran, IranDept. of Medical Mycology and Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran AND Invasive Fungi Research Center (IFRC), School of Medicine, Mazandaran University of Medical Sciences, Sari, IranDept. of Medical Mycology and Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran AND Invasive Fungi Research Center (IFRC), School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Background: Although Candida albicans remains the most common fungal isolate from clinical specimens, many studies have detected a shift towards non-albicans Candida species. Despite worrying clinical pictures associated with latter species, there is little information regarding its susceptibility patterns against currently available antifungal agents, with only a small number of strains having been studied. Methods: We evaluated the in vitro antifungal susceptibilities of clinical isolates of C. orthopsilosis already identified by two-steps PCR-RFLP and reconfirmed by sequence analysis of entire ITS rDNA region, to six antifungal drugs. Results: The resulting MIC50 and MIC90 for all strains (n=18) were in increasing order, as follows: posaconazole (0.016 & 0.063 μg/ml); itraconazole (0.031 & 0.125 μg/ml); amphotericin B (0.5 & 1 μg/ml); fluconazole (0.25 & 0.5 μg/ml) and caspofungin (4 & 8 μg/ml). A uniform pattern of the MIC ranges was seen for amphotericin B, fluconazole, itraconazole, and posaconazole, while a widest range and the highest MICs were observed for caspofungin. Conclusion: Although we emphasis on the careful species designation of the clinical isolates of Candida, the antifungal susceptibility patterns of these clinically important organisms may have an application in clinical and epidemiological setting and deserve the implementation of local surveillance programs to monitor. https://ijph.tums.ac.ir/index.php/ijph/article/view/9054Candida orthopsilosisOnychomycosisAntifungal susceptibilityIran |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rasoul MOHAMMADI Hossein MIRHENDI Mohammad Taghi HEDAYATI Hamid BADALI |
spellingShingle |
Rasoul MOHAMMADI Hossein MIRHENDI Mohammad Taghi HEDAYATI Hamid BADALI Caspofungin-Non-Susceptible Candida orthopsilosis Isolated from Onychomycosis in Iran Iranian Journal of Public Health Candida orthopsilosis Onychomycosis Antifungal susceptibility Iran |
author_facet |
Rasoul MOHAMMADI Hossein MIRHENDI Mohammad Taghi HEDAYATI Hamid BADALI |
author_sort |
Rasoul MOHAMMADI |
title |
Caspofungin-Non-Susceptible Candida orthopsilosis Isolated from Onychomycosis in Iran |
title_short |
Caspofungin-Non-Susceptible Candida orthopsilosis Isolated from Onychomycosis in Iran |
title_full |
Caspofungin-Non-Susceptible Candida orthopsilosis Isolated from Onychomycosis in Iran |
title_fullStr |
Caspofungin-Non-Susceptible Candida orthopsilosis Isolated from Onychomycosis in Iran |
title_full_unstemmed |
Caspofungin-Non-Susceptible Candida orthopsilosis Isolated from Onychomycosis in Iran |
title_sort |
caspofungin-non-susceptible candida orthopsilosis isolated from onychomycosis in iran |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Public Health |
issn |
2251-6085 2251-6093 |
publishDate |
2017-02-01 |
description |
Background: Although Candida albicans remains the most common fungal isolate from clinical specimens, many studies have detected a shift towards non-albicans Candida species. Despite worrying clinical pictures associated with latter species, there is little information regarding its susceptibility patterns against currently available antifungal agents, with only a small number of strains having been studied.
Methods: We evaluated the in vitro antifungal susceptibilities of clinical isolates of C. orthopsilosis already identified by two-steps PCR-RFLP and reconfirmed by sequence analysis of entire ITS rDNA region, to six antifungal drugs.
Results: The resulting MIC50 and MIC90 for all strains (n=18) were in increasing order, as follows: posaconazole (0.016 & 0.063 μg/ml); itraconazole (0.031 & 0.125 μg/ml); amphotericin B (0.5 & 1 μg/ml); fluconazole (0.25 & 0.5 μg/ml) and caspofungin (4 & 8 μg/ml). A uniform pattern of the MIC ranges was seen for amphotericin B, fluconazole, itraconazole, and posaconazole, while a widest range and the highest MICs were observed for caspofungin.
Conclusion: Although we emphasis on the careful species designation of the clinical isolates of Candida, the antifungal susceptibility patterns of these clinically important organisms may have an application in clinical and epidemiological setting and deserve the implementation of local surveillance programs to monitor.
|
topic |
Candida orthopsilosis Onychomycosis Antifungal susceptibility Iran |
url |
https://ijph.tums.ac.ir/index.php/ijph/article/view/9054 |
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