Cytotoxicity of Marchantia convoluta leaf extracts to human liver and lung cancer cells
The cytotoxicity of three extracts (petroleum ether, ethyl acetate and n-butanol) from a plant used in folk medicine, Marchantia convoluta, to human non-small cell lung carcinoma (H1299) and liver carcinoma (HepG2) cell lines was tested. After 72-h incubation of lung and liver cancer cell cultures w...
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Associação Brasileira de Divulgação Científica
2006-01-01
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doaj-3c47738353684220b815f1aafac779162020-11-24T23:46:07ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X0034-73102006-01-01396731738Cytotoxicity of Marchantia convoluta leaf extracts to human liver and lung cancer cellsXiao J.B.Chen X.Q.Zhang Y.W.Jiang X.Y.Xu M.The cytotoxicity of three extracts (petroleum ether, ethyl acetate and n-butanol) from a plant used in folk medicine, Marchantia convoluta, to human non-small cell lung carcinoma (H1299) and liver carcinoma (HepG2) cell lines was tested. After 72-h incubation of lung and liver cancer cell cultures with varying concentrations of extracts (15 to 200 µg/mL), cytotoxicity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and reported in terms of cell viability. The extracts that showed a significant cytotoxicity were subjected to gas chromatography-mass spectrometry analysis to identify the components. The ethyl acetate, but not the petroleum ether or n-butanol extract, had a significant cytotoxicity against lung and liver carcinoma cells with IC50 values of 100 and 30 µg/mL, respectively. A high concentration of ethyl acetate extract (100 µg/mL) rapidly reduced the number of H1299 cells. At lower concentrations of ethyl acetate extract (15, 30, and 40 µg/mL), the numbers of HepG2 cells started to decrease markedly. Gas chromatography-mass spectrometry analysis of the ethyl acetate extract revealed the presence of several compounds such as phytol (23.42%), 1,2,4-tripropylbenzene (13.09%), 9-cedranone (12.75%), ledene oxide (7.22%), caryophyllene (1.82%), and caryophyllene oxide (1.15%). HPLC analysis result showed that there were no flavonoids in ethyl acetate extract, but flavonoids are abundant in n-butanol extract. Further studies are needed regarding the identification, toxicity, and mechanism of action of active compounds.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600005Marchantia convoluta extractCytotoxicity assayHepG2 and H1299 carcinoma cell linesPhytolCaryophyllene oxide |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiao J.B. Chen X.Q. Zhang Y.W. Jiang X.Y. Xu M. |
spellingShingle |
Xiao J.B. Chen X.Q. Zhang Y.W. Jiang X.Y. Xu M. Cytotoxicity of Marchantia convoluta leaf extracts to human liver and lung cancer cells Brazilian Journal of Medical and Biological Research Marchantia convoluta extract Cytotoxicity assay HepG2 and H1299 carcinoma cell lines Phytol Caryophyllene oxide |
author_facet |
Xiao J.B. Chen X.Q. Zhang Y.W. Jiang X.Y. Xu M. |
author_sort |
Xiao J.B. |
title |
Cytotoxicity of Marchantia convoluta leaf extracts to human liver and lung cancer cells |
title_short |
Cytotoxicity of Marchantia convoluta leaf extracts to human liver and lung cancer cells |
title_full |
Cytotoxicity of Marchantia convoluta leaf extracts to human liver and lung cancer cells |
title_fullStr |
Cytotoxicity of Marchantia convoluta leaf extracts to human liver and lung cancer cells |
title_full_unstemmed |
Cytotoxicity of Marchantia convoluta leaf extracts to human liver and lung cancer cells |
title_sort |
cytotoxicity of marchantia convoluta leaf extracts to human liver and lung cancer cells |
publisher |
Associação Brasileira de Divulgação Científica |
series |
Brazilian Journal of Medical and Biological Research |
issn |
0100-879X 0034-7310 |
publishDate |
2006-01-01 |
description |
The cytotoxicity of three extracts (petroleum ether, ethyl acetate and n-butanol) from a plant used in folk medicine, Marchantia convoluta, to human non-small cell lung carcinoma (H1299) and liver carcinoma (HepG2) cell lines was tested. After 72-h incubation of lung and liver cancer cell cultures with varying concentrations of extracts (15 to 200 µg/mL), cytotoxicity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and reported in terms of cell viability. The extracts that showed a significant cytotoxicity were subjected to gas chromatography-mass spectrometry analysis to identify the components. The ethyl acetate, but not the petroleum ether or n-butanol extract, had a significant cytotoxicity against lung and liver carcinoma cells with IC50 values of 100 and 30 µg/mL, respectively. A high concentration of ethyl acetate extract (100 µg/mL) rapidly reduced the number of H1299 cells. At lower concentrations of ethyl acetate extract (15, 30, and 40 µg/mL), the numbers of HepG2 cells started to decrease markedly. Gas chromatography-mass spectrometry analysis of the ethyl acetate extract revealed the presence of several compounds such as phytol (23.42%), 1,2,4-tripropylbenzene (13.09%), 9-cedranone (12.75%), ledene oxide (7.22%), caryophyllene (1.82%), and caryophyllene oxide (1.15%). HPLC analysis result showed that there were no flavonoids in ethyl acetate extract, but flavonoids are abundant in n-butanol extract. Further studies are needed regarding the identification, toxicity, and mechanism of action of active compounds. |
topic |
Marchantia convoluta extract Cytotoxicity assay HepG2 and H1299 carcinoma cell lines Phytol Caryophyllene oxide |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600005 |
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