Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (<i>Gallus gallus</i>)

Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (<i>Gallus gallus</i>)

Chicken (<i>Gallus gallus</i>) pluripotent embryonic stem cells (ESCs) and primordial germ cells (PGCs) can be broadly applied in the research of developmental and embryonic biology, but the difference between amphoteric ESCs and PGCs is still elusive. This study determined the sex of co...

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Main Authors: Kai Jin, Jing Zhou, Qisheng Zuo, Jiuzhou Song, Yani Zhang, Guobing Chang, Guohong Chen, Bichun Li
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:Animals
Subjects:
differentially expressed genes
transcriptome analysis
amphoteric ESCs
amphoteric PGCs
chicken (<i>Gallus gallus</i>)
Online Access:https://www.mdpi.com/2076-2615/10/12/2228
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spelling doaj-3c4f626a11d4453c80bfeef5b67a1c542020-11-28T00:05:16ZengMDPI AGAnimals2076-26152020-11-01102228222810.3390/ani10122228Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (<i>Gallus gallus</i>)Kai Jin0Jing Zhou1Qisheng Zuo2Jiuzhou Song3Yani Zhang4Guobing Chang5Guohong Chen6Bichun Li7Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaAnimal & Avian Sciences, University of Maryland, College Park, MD 20741, USAKey Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaChicken (<i>Gallus gallus</i>) pluripotent embryonic stem cells (ESCs) and primordial germ cells (PGCs) can be broadly applied in the research of developmental and embryonic biology, but the difference between amphoteric ESCs and PGCs is still elusive. This study determined the sex of collected samples by identifying specific sex markers via polymerase chain reaction (PCR) and fluorescence activated cell sorter (FACS). RNA-seq was utilized to investigate the transcriptomic profile of amphoteric ESCs and PGCs in chicken. The results showed no significant differentially expressed genes (DEGs) in amphoteric ESCs and 227 DEGs exhibited in amphoteric PGCs. Moreover, those 227 DEGs were mainly enriched in 17 gene ontology (GO) terms and 27 pathways according to Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Furthermore, qRT-PCR was performed to verify RNA-seq results, and the results demonstrated that Notch1 was highly expressed in male PGCs. In summary, our results provided a knowledge base of chicken amphoteric ESCs and PGCs, which is helpful for future research in relevant biological processes.https://www.mdpi.com/2076-2615/10/12/2228differentially expressed genestranscriptome analysisamphoteric ESCsamphoteric PGCschicken (<i>Gallus gallus</i>)
collection DOAJ
language English
format Article
sources DOAJ
author Kai Jin
Jing Zhou
Qisheng Zuo
Jiuzhou Song
Yani Zhang
Guobing Chang
Guohong Chen
Bichun Li
spellingShingle Kai Jin
Jing Zhou
Qisheng Zuo
Jiuzhou Song
Yani Zhang
Guobing Chang
Guohong Chen
Bichun Li
Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (<i>Gallus gallus</i>)
Animals
differentially expressed genes
transcriptome analysis
amphoteric ESCs
amphoteric PGCs
chicken (<i>Gallus gallus</i>)
author_facet Kai Jin
Jing Zhou
Qisheng Zuo
Jiuzhou Song
Yani Zhang
Guobing Chang
Guohong Chen
Bichun Li
author_sort Kai Jin
title Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (<i>Gallus gallus</i>)
title_short Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (<i>Gallus gallus</i>)
title_full Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (<i>Gallus gallus</i>)
title_fullStr Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (<i>Gallus gallus</i>)
title_full_unstemmed Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (<i>Gallus gallus</i>)
title_sort transcriptome sequencing and comparative analysis of amphoteric escs and pgcs in chicken (<i>gallus gallus</i>)
publisher MDPI AG
series Animals
issn 2076-2615
publishDate 2020-11-01
description Chicken (<i>Gallus gallus</i>) pluripotent embryonic stem cells (ESCs) and primordial germ cells (PGCs) can be broadly applied in the research of developmental and embryonic biology, but the difference between amphoteric ESCs and PGCs is still elusive. This study determined the sex of collected samples by identifying specific sex markers via polymerase chain reaction (PCR) and fluorescence activated cell sorter (FACS). RNA-seq was utilized to investigate the transcriptomic profile of amphoteric ESCs and PGCs in chicken. The results showed no significant differentially expressed genes (DEGs) in amphoteric ESCs and 227 DEGs exhibited in amphoteric PGCs. Moreover, those 227 DEGs were mainly enriched in 17 gene ontology (GO) terms and 27 pathways according to Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Furthermore, qRT-PCR was performed to verify RNA-seq results, and the results demonstrated that Notch1 was highly expressed in male PGCs. In summary, our results provided a knowledge base of chicken amphoteric ESCs and PGCs, which is helpful for future research in relevant biological processes.
topic differentially expressed genes
transcriptome analysis
amphoteric ESCs
amphoteric PGCs
chicken (<i>Gallus gallus</i>)
url https://www.mdpi.com/2076-2615/10/12/2228
work_keys_str_mv AT kaijin transcriptomesequencingandcomparativeanalysisofamphotericescsandpgcsinchickenigallusgallusi
AT jingzhou transcriptomesequencingandcomparativeanalysisofamphotericescsandpgcsinchickenigallusgallusi
AT qishengzuo transcriptomesequencingandcomparativeanalysisofamphotericescsandpgcsinchickenigallusgallusi
AT jiuzhousong transcriptomesequencingandcomparativeanalysisofamphotericescsandpgcsinchickenigallusgallusi
AT yanizhang transcriptomesequencingandcomparativeanalysisofamphotericescsandpgcsinchickenigallusgallusi
AT guobingchang transcriptomesequencingandcomparativeanalysisofamphotericescsandpgcsinchickenigallusgallusi
AT guohongchen transcriptomesequencingandcomparativeanalysisofamphotericescsandpgcsinchickenigallusgallusi
AT bichunli transcriptomesequencingandcomparativeanalysisofamphotericescsandpgcsinchickenigallusgallusi
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