Immunochromatographic paper sensor for ultrasensitive colorimetric detection of cadmium

• Main Authors: Shanshan Song, Shuzhen Zou, Jianping Zhu, Liqiang Liu, Hua Kuang
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2018-01-01
• Series: Food and Agricultural Immunology
• Subjects: cadmium; immunochromatographic strip; monoclonal antibody; gold-nanoparticle (gnp)
• Online Access: http://dx.doi.org/10.1080/09540105.2017.1354358

A novel and highly sensitive immunochromatographic strip based on a monoclonal antibody (3A9) was developed for the detection of cadmium in tap water. The 50% inhibition concentration of the antibody, which showed no cross-reactivity with other heavy metal ions, was 0.45 ng/mL and it recognized Cd(II)–1-(4-isothiocyanobenzyl) ethylenediamine-N,N,N′,N′-tetraacetic acid (EDTA) (Cd(II)–ITCBE) and not metal-free ITCBE and EDTA. The cutoff value for semi-quantitative detection of the strip was 5 ng/mL and the lower limit of detection for quantitative detection was 0.2 ng/mL using a scanning reader. The percent recovery ranged from 107.6% to 132% in tap water samples.