In vitro cytotoxicity of orthodontic elastics: comparison between two methodologies

In vitro cytotoxicity of orthodontic elastics: comparison between two methodologies

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The aim of this study was to compare two methodologies, evaluating in vitro the biocompatibility of intra-oral orthodontic elastics through cytotoxity test in culture of HEp-2 cells (human larynx carcinoma), comparing two methodologies: diffusion in agar and incorporation of the neutral red assay....

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Main Authors: Matheus Melo Pithon, Rogério Lacerda dos Santos, Antônio Carlos de Oliveira Ruellas, Matilde da Cunha Goncalves Nojima, Tatiana Kelly da Silva Fidalgo, Maria Teresa Villela Romanos, Gabriella da Silva Mendes
Format: Article
Language:Portuguese
Published: Universidade Estadual do Sudoeste da Bahia 2008-01-01
Series:Revista Saúde.com
Subjects:
cytotoxicity
neutral red uptake methodology
agar diffusion test
cell culture
Online Access:http://www.uesb.br/revista/rsc/v4/v4n1a03.pdf
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spelling doaj-3c5af0ac9db34a83999112fc7ab3495f2020-11-24T22:33:41ZporUniversidade Estadual do Sudoeste da BahiaRevista Saúde.com1809-07612008-01-01411926In vitro cytotoxicity of orthodontic elastics: comparison between two methodologiesMatheus Melo PithonRogério Lacerda dos SantosAntônio Carlos de Oliveira RuellasMatilde da Cunha Goncalves NojimaTatiana Kelly da Silva FidalgoMaria Teresa Villela RomanosGabriella da Silva MendesThe aim of this study was to compare two methodologies, evaluating in vitro the biocompatibility of intra-oral orthodontic elastics through cytotoxity test in culture of HEp-2 cells (human larynx carcinoma), comparing two methodologies: diffusion in agar and incorporation of the neutral red assay. Orthodontic elastics of two different commercial labels were used: American Orthodontic (American Orthodontic, Sheboygon, the USA) and Morelli (Sorocaba, São Paulo, Brazil). The results using both methodologies demonstrated low cytotoxity for American Orthodontic and for Morelli. In accordance with the results can be concluded that both methodologies can be used to test orthodontic elastics.http://www.uesb.br/revista/rsc/v4/v4n1a03.pdfcytotoxicityneutral red uptake methodologyagar diffusion testcell culture
collection DOAJ
language Portuguese
format Article
sources DOAJ
author Matheus Melo Pithon
Rogério Lacerda dos Santos
Antônio Carlos de Oliveira Ruellas
Matilde da Cunha Goncalves Nojima
Tatiana Kelly da Silva Fidalgo
Maria Teresa Villela Romanos
Gabriella da Silva Mendes
spellingShingle Matheus Melo Pithon
Rogério Lacerda dos Santos
Antônio Carlos de Oliveira Ruellas
Matilde da Cunha Goncalves Nojima
Tatiana Kelly da Silva Fidalgo
Maria Teresa Villela Romanos
Gabriella da Silva Mendes
In vitro cytotoxicity of orthodontic elastics: comparison between two methodologies
Revista Saúde.com
cytotoxicity
neutral red uptake methodology
agar diffusion test
cell culture
author_facet Matheus Melo Pithon
Rogério Lacerda dos Santos
Antônio Carlos de Oliveira Ruellas
Matilde da Cunha Goncalves Nojima
Tatiana Kelly da Silva Fidalgo
Maria Teresa Villela Romanos
Gabriella da Silva Mendes
author_sort Matheus Melo Pithon
title In vitro cytotoxicity of orthodontic elastics: comparison between two methodologies
title_short In vitro cytotoxicity of orthodontic elastics: comparison between two methodologies
title_full In vitro cytotoxicity of orthodontic elastics: comparison between two methodologies
title_fullStr In vitro cytotoxicity of orthodontic elastics: comparison between two methodologies
title_full_unstemmed In vitro cytotoxicity of orthodontic elastics: comparison between two methodologies
title_sort in vitro cytotoxicity of orthodontic elastics: comparison between two methodologies
publisher Universidade Estadual do Sudoeste da Bahia
series Revista Saúde.com
issn 1809-0761
publishDate 2008-01-01
description The aim of this study was to compare two methodologies, evaluating in vitro the biocompatibility of intra-oral orthodontic elastics through cytotoxity test in culture of HEp-2 cells (human larynx carcinoma), comparing two methodologies: diffusion in agar and incorporation of the neutral red assay. Orthodontic elastics of two different commercial labels were used: American Orthodontic (American Orthodontic, Sheboygon, the USA) and Morelli (Sorocaba, São Paulo, Brazil). The results using both methodologies demonstrated low cytotoxity for American Orthodontic and for Morelli. In accordance with the results can be concluded that both methodologies can be used to test orthodontic elastics.
topic cytotoxicity
neutral red uptake methodology
agar diffusion test
cell culture
url http://www.uesb.br/revista/rsc/v4/v4n1a03.pdf
work_keys_str_mv AT matheusmelopithon invitrocytotoxicityoforthodonticelasticscomparisonbetweentwomethodologies
AT rogeriolacerdadossantos invitrocytotoxicityoforthodonticelasticscomparisonbetweentwomethodologies
AT antoniocarlosdeoliveiraruellas invitrocytotoxicityoforthodonticelasticscomparisonbetweentwomethodologies
AT matildedacunhagoncalvesnojima invitrocytotoxicityoforthodonticelasticscomparisonbetweentwomethodologies
AT tatianakellydasilvafidalgo invitrocytotoxicityoforthodonticelasticscomparisonbetweentwomethodologies
AT mariateresavillelaromanos invitrocytotoxicityoforthodonticelasticscomparisonbetweentwomethodologies
AT gabrielladasilvamendes invitrocytotoxicityoforthodonticelasticscomparisonbetweentwomethodologies
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